EDG1 (S1PR1) (NM_001400) Human Untagged Clone

CAT#: SC119262

S1PR1 (untagged)-Human sphingosine-1-phosphate receptor 1 (S1PR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S1PR1
• Synonyms: CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; S1P1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001400 edited
  ATGGGGCCCACCAGCGTCCCGCTGGTCAAGGCCCACCGCAGCTCGGTCTCTGACTACGTC
  AACTATGATATCATCGTCCGGCATTACAACTACACGGGAAAGCTGAATATCAGCGCGGAC
  AAGGAGAACAGCATTAAACTGACCTCGGTGGTGTTCATTCTCATCTGCTGCTTTATCATC
  CTGGAGAACATCTTTGTCTTGCTGACCATTTGGAAAACCAAGAAATTCCACCGACCCATG
  TACTATTTTATTGGCAATCTGGCCCTCTCAGACCTGTTGGCAGGAGTAGCCTACACAGCT
  AACCTGCTCTTGTCTGGGGCCACCACCTACAAGCTCACTCCCGCCCAGTGGTTTCTGCGG
  GAAGGGAGTATGTTTGTGGCCCTGTCAGCCTCCGTGTTCAGTCTCCTCGCCATCGCCATT
  GAGCGCTATATCACAATGCTGAAAATGAAACTCCACAACGGGAGCAATAACTTCCGCCTC
  TTCCTGCTAATCAGCGCCTGCTGGGTCATCTCCCTCATCCTGGGTGGCCTGCCTATCATG
  GGCTGGAACTGCATCAGTGCGCTGTCCAGCTGCTCCACCGTGCTGCCGCTCTACCACAAG
  CACTATATCCTCTTCTGCACCACGGTCTTCACTCTGCTTCTGCTCTCCATCGTCATTCTG
  TACTGCAGAATCTACTCCTTGGTCAGGACTCGGAGCCGCCGCCTGACGTTCCGCAAGAAC
  ATTTCCAAGGCCAGCCGCAGCTCTGAGAAGTCGCTGGCGCTGCTCAAGACCGTAATTATC
  GTCCTGAGCGTCTTCATCGCCTGCTGGGCACCGCTCTTCATCCTGCTCCTGCTGGATGTG
  GGCTGCAAGGTGAAGACCTGTGACATCCTCTTCAGAGCGGAGTACTTCCTGGTGTTAGCT
  GTGCTCAACTCCGGCACCAACCCCATCATTTACACTCTGACCAACAAGGAGATGCGTCGG
  GCCTTCATCCGGATCATGTCCTGCTGCAAGTGCCCGAGCGGAGACTCTGCTGGCAAATTC
  AAGCGACCCATCATCGCCGGCATGGAATTCAGCCGCAGCAAATCGGACAATTCCTCCCAC
  CCCCAGAAAGACGAAGGGGACAACCCAGAGACCATTATGTCTTCTGGAAACGTCAACTCT
  TCTTCCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001400 unedited
  GTTTAGAATTGTATACGACTCCTATAGGCGGCCGCGNAATCGGCACGAGGCGCCACCCCG
  GCTTCCTGGGGACACAGGGTTGGCACCATGGGGCCCACCAGCGTCCCGCTGGTCAAGGCC
  CACCGCAGCTCGGTCTCTGACTACGTCAACTATGATATCATCGTCCGGCATTACAACTAC
  ACGGGAAAGCTGAATATCAGCGCGGACAAGGAGAACAGCATTAAACTGACCTCGGTGGTG
  TTCATTCTCATCTGCTGCTTTATCATCCTGGAGAACATCTTTGTCTTGCTGACCATTTGG
  AAAACCAAGAAATTCCACCGACCCATGTACTATTTTATTGGCAATCTGGCCCTCTCAGAC
  CTGTTGGCAGGAGTAGCCTACACAGCTAACCTGCTCTTGTCTGGGGCCACCACCTACAAG
  CTCACTCCCGCCCAGTGGTTTCTGCGGGAAGGGAGTATGTTTGTGGCCCTGTCAGCCTCC
  GTGTTCAGTCTCCTCGCCATCGCCATTGAGCGCTATATCACAATGCTGAAAATGAAACTC
  CACAACGGGAGCAATAACTTCCGCCTCTTCCTGCTAATCAGCGCCTGCTGGGTCATCTCC
  CTCATCCTGGGTGGCCTGCCTATCATGGGCTGGAACTGCATCAGTGCGCTGTCCAGCTGC
  TCCACCGTGCTGCCGCTCTACCACAAGCACTATATCCTCTTCTGCACCACGGTCTTCACT
  CTGCTTCTGCTCTCCATCGTCATTCTGTACTGCAGAATCTACTCCTTGGTCANGACTCGG
  AGCCGCCGCCTGACGTTCCCGCAGAACATTTCCAAGGCCAGCCGCAGCTCTGAGAAGTCG
  CTGGCGCTGCTCAAGACGTAATTATCGTCCTGAGCGTCCTCATCGCCTGCTGGGCACCGT
  TCTTCATCCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001400 unedited
  AAATAATTTTTATGTTTAAAGTTGATAAAGCTATGTGCAAAATGATCCACAAAAGTCAGA
  AACCTATTAAATACTATTCTTTTTAAAAAAACAAGACATCTCTCGGTTTAATTGCACNTG
  AAAACCATACTAAAGAGACAGTAATATATTTTTATTCTTTGTAATATTTATACATATCTT
  CAATTACTATCTAATGAATAGCTGGACTGAAAAGTTTCTGGCGTTCCCTACCAGTTAAAG
  TAATGCTTTTAAGGGCACAAGAGGGTGGTATTTTCTTAAGAAATACACATTCAATGGTGT
  TAACACGGGTTAGAAAAATCCAATAAAATGACTTCTGCTGAAACAAATACATTCAAAAAT
  CAAGAAAAGTACTTTTAACACAACAAAATACAACCATGTTGTTGCTTGAAATGAATCACA
  CAACATTCTGAAATAAACATCAACAAAAGTGCCAAAGACAGATGTCATATTTGTTAGACT
  CATTTGCTAAGAAATCTCCCTTGGTTTGCAAAAGTTAATCCATTCGGTTTTCACTTTGTT
  TTGCTTGTCTGTTTCTCCTAGGACACCAAGGATCCTGGCTAATATCATTTAGATAAAGTG
  GGCTTCCTTATGCAGATTTCGGCTTCAATGGATATGAAATGGTAACACATTTCATTGAAA
  AGATGTTTTTTAAAGAAATCAAAGTGACTTCAACCAAATTCCAANAACGGATCCCGCTTA
  CATGGAAACTTTGGCCTCAGCGAAGGACATTGTTTTACACCTCCAAACATCTTCATCTTT
  CACAGCCTACGTACTCACATAGGCTATTTGCTATGATGAACGATCATCCACAACCCCACC
  TTTNTAACTTTCAGGTGGTTAAAGATAAAGGAAAAAAACCTNAGGAGGGTTGAAAGAAGG
  AGGGCNNGNGTTTTCTAATTACAAGCATCCCCAAACACCNNNNCTACACTCACTTTNTNN
  AACTAACGAGCGAACCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001400
• Insert Size: 2590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001400.2, NP_001391.2
• RefSeq Size: 2753 bp
• RefSeq ORF: 1149 bp
• Locus ID: 1901
• Cytogenetics: 1p21.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.