EDG2 (LPAR1) (NM_001401) Human Untagged Clone

CAT#: SC119263

LPAR1 (untagged)-Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LPAR1
• Synonyms: edg-2; EDG2; Gpcr26; GPR26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119263 sequence for NM_001401 edited (data generated by NextGen Sequencing)
  ATGGCTGCCATCTCTACTTCCATCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAAT
  GAACCACAGTGCTTCTACAACGAGTCCATTGCCTTCTTTTATAACCGAAGTGGAAAGCAT
  CTTGCCACAGAATGGAACACAGTCAGCAAGCTGGTGATGGGACTTGGAATCACTGTTTGT
  ATCTTCATCATGTTGGCCAACCTATTGGTCATGGTGGCAATCTATGTCAACCGCCGCTTC
  CATTTTCCTATTTATTACCTAATGGCTAATCTGGCTGCTGCAGACTTCTTTGCTGGGTTG
  GCCTACTTCTATCTCATGTTCAACACAGGACCCAATACTCGGAGACTGACTGTTAGCACA
  TGGCTCCTTCGTCAGGGCCTCATTGACACCAGCCTGACGGCATCTGTGGCCAACTTACTG
  GCTATTGCAATCGAGAGGCACATTACGGTTTTCCGCATGCAGCTCCACACACGGATGAGC
  AACCGGCGGGTAGTGGTGGTCATTGTGGTCATCTGGACTATGGCCATCGTTATGGGTGCT
  ATACCCAGTGTGGGCTGGAACTGTATCTGTGATATTGAAAATTGTTCCAACATGGCACCC
  CTCTACAGTGACTCTTACTTAGTCTTCTGGGCCATTTTCAACTTGGTGACCTTTGTGGTA
  ATGGTGGTTCTCTATGCTCACATCTTTGGCTATGTTCGCCAGAGGACTATGAGAATGTCT
  CGGCATAGTTCTGGACCCCGGCGGAATCGGGATACCATGATGAGTCTTCTGAAGACTGTG
  GTCATTGTGCTTGGGGCCTTTATCATCTGCTGGACTCCTGGATTGGTTTTGTTACTTCTA
  GACGTGTGCTGTCCACAGTGCGACGTGCTGGCCTATGAGAAATTCTTCCTTCTCCTTGCT
  GAATTCAACTCTGCCATGAACCCCATCATTTACTCCTACCGCGACAAAGAAATGAGCGCC
  ACCTTTAGGCAGATCCTCTGCTGCCAGCGCAGTGAGAACCCCACCGGCCCCACAGAAGGC
  TCAGACCGCTCGGCTTCCTCCCTCAACCACACCATCTTGGCTGGAGTTCACAGCAATGAC
  CACTCTGTGGTTTAG
  
  Clone variation with respect to NM_001401.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001401 unedited
  GGGTTCACATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGCTTC
  TCCTAGCATGACTTCGATCTGATCAGCAAACAAGAAAATTGTCTCCCGTAGTTCTGGGGC
  GTGTTCACCACCTACAACCACAGAGCTGTCATGGCTGCCATCTCTACTTCCATCCCTGTA
  ATTTCACAGCCCCAGTTCACAGCCATGAATGAACCACAGTGCTTCTACAACGAGTCCATT
  GCCTTCTTTTATAACCGAAGTGGAAAGCATCTTGCCACAGAATGGAACACAGTCAGCAAG
  CTGGTGATGGGACTTGGAATCACTGTTTGTATCTTCATCATGTTGGCCAACCTATTGGTC
  ATGGTGGCAATCTATGTCAACCGCCGCTTCCATTTTCCTATTTATTACCTAATGGCTAAT
  CTGGCTGCTGCAGACTTCTTTGCTGGGTTGGCCTACTTCTATCTCATGTTCAACACAGGA
  CCCAATACTCGGAGACTGACTGTTAGCACATGGCTCCTTCGTCAGGGCCTCATTGACACC
  AGCCTGACGGCATCTGTGGCCAACTTACTGGCTATTGCAATCGAGAGGACTTTTACGGGT
  TTCCGCATGCAGCTCCACACACGGATGAGCAACCGGCGGGTAGTGGTGGTCATTGTGGTC
  ATCTGGACTATGGCCATCGTTATGGGTGCTATACCCAGTGTGGGCTGGGACTGTATCTGT
  GATATTGAAAATTGTTCCAACATGGCACCCCTCTACAGTGACTCTTACTTAGTCTTCTGG
  GCCATTTTCAACTTGGTGACCTTTGTGGAAAGGGGGGGTCTCTATGCTCACACTCTTGGC
  TATGGTCGCCAGAGAACATGAGAATGTCTCGGATAATTCTGGACCCCGCCCATCGGGAAA
  CATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001401 unedited
  CGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGATATGACAATCCTTTATTC
  ACTCGGCACATTTGGTTTCTTTGCATTTTCTTCCATTTTACATTGCAGGTGTGGCTACCA
  AGAGCTGGATAACGAGTCCCTCAAACAAAGTTTGGAATTGCGAGATATATTGGGGTACCT
  TGATTCTTGAGACAGTTGAATTCTTCTAGTTGATCTTGTTGTATTAAAAAGTCACTCTCA
  ACTGAAGTGACCACTGCATTTCTTTTGTAAAAAGGTCATTTGACTGGCTTTTCCTCACAA
  CTGCCACCCATGCAGTACAAAAGCAGGAAATCCCTTTGCATGAAAGGTGTAAGTACAAGA
  TGACATTTCACATTTTTTTAAAAAAAGAATCCTTCATGGGAATATATCCTAATAATCAAT
  TATATGGAGACAGTTTTATGTACACCAAATTTCTGCAACTTTATAATAATCAAAATTAGA
  AACAACCTAAATAGTTAACAACATGGGAATGGTTAAATAAACTGAGTTGCATCCATTAAA
  TGGAATATAATATAGCCATTAAAATTATGTTTTTGTAAAATTTTTAATGCCATAAGAAAA
  TGTGGCAATTTTGCAATGAAAAAGATCTACTTATAAAACTGTTTACAGTATGACTCCAAT
  TATGTAAAAAAAGTATACAATACACATATAGGCCTACATGGGGGTTGCTTTTTAAGGAGG
  CTACTTCTGGGTTGCGATATTATAGAAATATTTTGCCTTTTATACATTNCGTATTTTTCA
  AGTTTCTTGATGAGTTATTATTTTCAAAGACTACCAATTTTCCTCTGAATACTGGAATTA
  AATGTACCTGGGATTTTTAATTATGGGAACAACTTTAGGCTTCTCTTCTTGACTTTTAGA
  TAACTACACTTGGGATAAACTCCTTCCTGTTTAACTGGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001401
• Insert Size: 2910 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001401.3, NP_001392.2
• RefSeq Size: 3104 bp
• RefSeq ORF: 1095 bp
• Locus ID: 1902
• Cytogenetics: 9q31.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Gap junction, Neuroactive ligand-receptor interaction
• Gene Summary: 'The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the shorter transcript. Variants 1-26 encode the same protein.