EEF1G (NM_001404) Human Untagged Clone

CAT#: SC119266

EEF1G (untagged)-Human eukaryotic translation elongation factor 1 gamma (EEF1G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EEF1G
• Synonyms: EF1G; GIG35
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001404, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTGGGACCCTGTACACGTATCCTGAAAACTGGAGGGCCTTCAAGGCTCTCATCGCTGCTCAGT
  ACAGCGGGGCTCAGGTCCGCGTGCTCTCCGCACCACCCCACTTCCATTTTGGCCAAACCAACCGCACCCC
  TGAATTTCTCCGCAAATTTCCTGCCGGCAAGGTCCCAGCATTTGAGGGTGATGATGGATTCTGTGTGTTT
  GAGAGCAACGCCATTGCCTACTATGTGAGCAATGAGGAGCTGCGGGGAAGTACTCCAGAGGCAGCAGCCC
  AGGTGGTGCAGTGGGTGAGCTTTGCTGATTCCGATATAGTGCCCCCAGCCAGTACCTGGGTGTTCCCCAC
  CTTGGGCATCATGCACCACAACAAACAGGCCACTGAGAATGCAAAGGAGGAAGTGAGGCGAATTCTGGGG
  CTGCTGGATGCTTACTTGAAGACGAGGACTTTTCTGGTGGGCGAACGAGTGACATTGGCTGACATCACAG
  TTGTCTGCACCCTGTTGTGGCTCTATAAGCAGGTTCTAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATAC
  CAACCGCTGGTTCCTCACCTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGTGT
  GAGAAGATGGCCCAGTTTGATGCTAAAAAGTTTGCAGAGACCCAACCTAAAAAGGACACACCACGGAAAG
  AGAAGGGTTCACGGGAAGAGAAGCAGAAGCCCCAGGCTGAGCGGAAGGAGGAGAAAAAGGCGGCTGCCCC
  TGCTCCTGAGGAGGAGATGGATGAATGTGAGCAGGCGCTGGCTGCTGAGCCCAAGGCCAAGGACCCCTTC
  GCTCACCTGCCCAAGAGTACCTTTGTGTTGGATGAATTTAAGCGCAAGTACTCCAATGAGGACACACTCT
  CTGTGGCACTGCCATATTTCTGGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCG
  CTTCCCTGAAGAACTCACTCAGACCTTCATGAGCTGCAATCTCATCACTGGAATGTTCCAGCGACTGGAC
  AAGCTGAGGAAGAATGCCTTCGCCAGTGTCATCCTTTTTGGAACCAACAATAGCAGCTCCATTTCTGGAG
  TCTGGGTCTTCCGAGGCCAGGAGCTTGCCTTTCCGCTGAGTCCAGATTGGCAGGTGGACTACGAGTCATA
  CACATGGCGGAAACTGGATCCTGGCAGCGAGGAGACCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGAG
  GGGGCCTTCCAGCATGTGGGCAAAGCCTTCAATCAGGGCAAGATCTTCAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001404 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGGCGGCTGGGACCCTGTACACG
  TATCCTGAAAACTGGAGGGCCTTCAAGGCTCTCATCGCTGCTCAGTACAGCGGGGCTCAG
  GTCCGCGTGCTCTCCGCACCACCCCACTTCCATTTTGGCCAAACCAACCGCACCCCTGAA
  TTTCTCCGCAAATTTCCTGCCGGCAAGGTCCCAGCATTTGAGGGTGATGATGGATTCTGT
  GTGTTTGAGAGCAACGCCATTGCCTACTATGTGAGCAATGAGGAGCTGCGGGGAAGTACT
  CCAGAGGCAGCAGCCCAGGTGGTGCAGTGGGTGAGCTTTGCTGATTCCGATATAGTGCCC
  CCAGCCAGTACCTGNGTGTTCCCCACCTTGGGCATCATGCACCACAACAAACAGGCCACT
  GAGAATGCAAAGGAGGAAGTGAGGCGAATTCTGGGGCTGCTGGATGCTTACTTGAAGACG
  AGGACTTTTCTGGTGGGCGAACGAGTGACATTGGCTGACATCACAGTTGTCTGCACCCTG
  TTGTGGCTCTATAAGCAGGTTCTAGAGCCTTCTTTCCGCCAGGCCTTTCCCATACCAACC
  GCTGGTTCCTCACCTGCATTAACCAGCCCCAGTTCCGGGNCTGTCTTGGGCGAAGTGAAA
  CTGTGTGAGAAGATGGCCCAGTTTGATGCTAAAAAGTTTGCAGAGACCCCACCTANNNAA
  GACACACCANCGAAAGAAANGGGTTCACGGGAAGAAGAGCANAAGCCCCCAGCTGAGCGN
  AAAGGAGAGAAAANGCNGNCTGCCCCTGCTCCCTGAGAGAAATGNATGATGTGAACAGCG
  CCTGCTGCTAAACCAAAGCAAGACCCNTNGCTCACTGCCAAGAGTCCCTTGGGTGGATGA
  ATTAGCGCAGTACTCATGAGACCACTCTTGGGGACTGCCTATCCTGGGACCTTGATAAGA
  CCGCTGCCCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001404 unedited
  GGGGNNNNTTACTTGAACCGCGCCGCATCTAGGATCGAGTNNTTTTTTTTTTTTTTTTTT
  TTTTTTACAATGTTCAGTTTCCTTTAATGACCCCCATCTCCCTGAAGGGCAGGTGCAGGC
  AGCTAGGTGATGGCAAAAGATGTTCACTTGAAAATCTTGCCCTGATTGAAGGCTTTGCCC
  ACATGCTGGAAGGCCCCCTCCCAGGAAAAGTACTCTCGAACCAGCGTCTGGGTCTCCTCG
  CTGCCAGGATCCAGTTTCCGCCATGTGTATGACTCGTAGTCCACCTGCCAATCTGGACTC
  AGCGGAAAGGCAAGTTCCTGGCCTCGGAAACCCAGACTNCAAAAATGGACCTGCTATTGT
  TGTTCCAAAAAGGATGACACTGGCAAAGGCATTTTTCCTCAGCTTGCCCATCGCTGAACA
  TTCAATGATGAAATGCCNCTATGAAAGCCTGAGTGAGTCTTTAGGAAGCCATCCTTTGGA
  TCCACAGGGCCAGCCGTCCTTATAAAAGGCTCCCAAATTTGGGTTGCCCCAAAAGGGGGC
  CTTTTTGAATACTTGCCCTTATTCTCCAACCAAAGGCCTTTTGGCCAGGAACAAGGGGTC
  TTGGCTGGGCTAAAAACCACCCTGGTCAATTTATCATTTCTCCTAAGGACGAGGGCACCC
  GCTTTTTTCTCTTTTCCTACACCTGGGGTTTGGTTTTTTCCGGAACCCTTTTTTTCCGGG
  GGGCCCTTTAAGGGGGGGCTTGAAAATTTTTAGAATAAAGGGGCCCTTTTCCACCGTTTC
  TTTCCCACAAAACCCCCAATGGGGGGGTTTCAGGGGAGAACCCCCCGGTGTTTTGAAAAG
  CCTTGCGAAAAAGGCCTAACCCCTTTTTACACCCCAAGGGGGCCCCCTTGGTTTTCCCCG
  TTTTTTTTCCCCCCAAAAATCCCTTTAAAATCCTCCCCCCCCCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001404
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001404.3, NP_001395.1
• RefSeq Size: 1429 bp
• RefSeq ORF: 1314 bp
• Locus ID: 1937
• Cytogenetics: 11q12.3
• Domains: EF1G_domain, GST_N, GST_C
• Gene Summary: 'This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008]'