Ephrin B3 (EFNB3) (NM_001406) Human Untagged Clone

CAT#: SC119267

EFNB3 (untagged)-Human ephrin-B3 (EFNB3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EFNB3
• Synonyms: EFL6; EPLG8; LERK8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001406 edited
  GCCTTAGCCCGCTGCCCTCAATCCCAGCGAGGCTGGGGCTCCGGCTCGGCGCCCCCTTCC
  TCGCTCCCTGGTCCGGCGCCCCATGCCGCCCCCGCCCGGTCCCCGGCTCCCCCAGTCCCC
  CACTTAGGCGGGCTCACAGATCCCGGGGTGCTGGCGCGTGGGCCGGGGGCGCGTAGGGCG
  CCTGCAGACGGCCCCTGGAAGGGCTCTGGTGGGGCTGAGCGCTCTGCCGCGGGGGCGCGG
  GCACAGCAGGAAGCAGGTCCGCGTGGGCGCTGGGGGCATCAGCTACCGGGGTGGTCCGGG
  CTGAAGAGCCAGGCAGCCAAGGCAGCCACCCCGGGGGGTGGGCGACTTTGGGGGAGTTGG
  TGCCCCGCCCCCCAGGCCTTGGCGGGGTCATGGGGCCCCCCCATTCTGGGCCGGGGGGCG
  TGCGAGTCGGGGCCCTGCTGCTGCTGGGGGTTTTGGGGCTGGTGTCTGGGCTCAGCCTGG
  AGCCTGTCTACTGGAACTCGGCGAATAAGAGGTTCCAGGCAGAGGGTGGTTATGTGCTGT
  ACCCTCAGATCGGGGACCGGCTAGACCTGCTCTGCCCCCGGGCCCGGCCTCCTGGCCCTC
  ACTCCTCTCCTAATTATGAGTTCTACAAGCTGTACCTGGTAGGGGGTGCTCAGGGCCGGC
  GCTGTGAGGCACCCCCTGCCCCAAACCTCCTTCTCACTTGTGATCGCCCAGACCTGGATC
  TCCGCTTCACCATCAAGTTCCAGGAGTATAGCCCTAATCTCTGGGGCCACGAGTTCCGCT
  CGCACCACGATTACTACATCATTGCCACATCGGATGGGACCCGGGAGGGCCTGGAGAGCC
  TGCAGGGAGGTGTGTGCCTAACCAGAGGCATGAAGGTGCTTCTCCGAGTGGGACAAAGTC
  CCCGAGGAGGGGCTGTCCCCCGAAAACCTGTGTCTGAAATGCCCATGGAAAGAGACCGAG
  GGGCAGCCCACAGCCTGGAGCCTGGGAAGGAGAACCTGCCAGGTGACCCCACCAGCAATG
  CAACCTCCCGGGGTGCTGAAGGCCCCCTGCCCCCTCCCAGCATGCCTGCAGTGGCTGGGG
  CAGCAGGGGGGCTGGCGCTGCTCTTGCTGGGCGTGGCAGGGGCTGGGGGTGCCATGTGTT
  GGCGGAGACGGCGGGCCAAGCCTTCGGAGAGTCGCCACCCTGGTCCTGGCTCCTTCGGGA
  GGGGAGGGTCTCTGGGCCTGGGGGGTGGAGGTGGGATGGGACCTCGGGAGGCTGAGCCTG
  GGGAGCTAGGGATAGCTCTGCGGGGTGGCGGGGCTGCAGATCCCCCCTTCTGCCCCCACT
  ATGAGAAGGTGAGTGGTGACTATGGGCATCCTGTGTATATCGTGCAGGATGGGCCCCCCC
  AGAGCCCTCCAAACATCTACTACAAGGTATGAGGGCTCCTCTCACGTGGCTATCCTGAAT
  CCAGCCCTTCTTGGGGTGCTCCTCCAGTTTAATTCCTGGTTTGAGGGACACCTCTAACAT
  CTCGGCCCCCTGTGCCCCCCCAGCCCCTTCACTCCTCCCGGCTGCTGTCCTCGTCTCCAC
  TTTTAGGATTCCTTAGGATTCCCACTGCCCCACTTCCTGCCCTCCCGTTTGGCCATGGGT
  GCCCCCCTCTGTCTCAGTGTCCCTGGATCCTTTTTCCTTGGGGAGGGGCACAGGCTCAGC
  CTCCTCTCTGACCATGACCCAGGCATCCTTGTCCCCCTCACCCACCCAGAGCTAGGGGCG
  GGAACAGCCCACCTTTTGGTTGGCACCGCCTTCTTTCTGCCTCTCACTGGTTTTCTCTTC
  TCTATCTCTTATTCTTTCCCTCTCTTCCGTCTCTAGGTCTGTTCTTCTTCCCTAGCATCC
  TCCTCCCCACATCTCCTTTCACCCTCTTGGCTTCTTATCCTGTGCCTCTCCCATCTCCTG
  GGTGGGGGCATCAAAGCATTTCTCCCCTTAGCTTTCAGCCCCCCTTCTGACCTCTCATAC
  CAACCACTCCCCTCAGTCTGCCAAAAATGGGGGCCTTATGGGGAAAGCTCTGACACTCCA
  CCCCAGCTCAGGCCATGGGCAGCAGGGCTCCATTCTCTGGGCCTGGGCCAAGCCTCTACA
  TACTTACTCCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001406 unedited
  TGTTCACATTTGTATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGCCTTAGCCC
  GCTGCCCTCAATCCCAGCGAGGCTGGGGCTCCGGCTCGGCGCCCCCTTCCTCGCTCCCTG
  GTCCGGCGCCCCATGCCGCCCCCGCCCGGTCCCCGGCTCCCCCAGTCCCCCACTTAGGCG
  GGCTCACAGATCCCGGGGTGCTGGCGCGTGGGCCGGGGGCGCGTAGGGCGCCTGCAGACG
  GCCCCTGGAAGGGCTCTGGTGGGGCTGAGCGCTCTGCCGCGGGGGCGCGGGCACAGCAGG
  AAGCAGGTCCGCGTGGGCGCTGGGGGCATCAGCTACCGGGGTGGTCCGGGCTGAAGAGCC
  AGGCAGCCAAGGCAGCCACCCCGGGGGGTGGGCGACTTTGGGGGAGTTGGTGCCCCGCCC
  CCCAGGCCTTGGCGGGGTCATGGGGCCCCCCCATTCTGGGCCGGGGGGCGTGCGAGTCGG
  GGCCCTGCTGCTGCTGGGGGTTTTGGGGCTGGTGTCTGGGCTCAGCCTGGAGCCTGTCTA
  CTGGAACTCGGCGAATAAGAGGTTCCAGGCAGAGGGTGGTTATGTGCTGTACCCTCAGAT
  CGGGGACCGGCTAGACCTGCTCTGCCCCCGGGCCCGGCCTCCTGGCCCTCACTCCTCTCC
  TAATTATGAGTTCTACAAGCTGTACCTGTAAGGGGGGTGCTCANGGNCCGGCGCTGTGAT
  GCACCCCCTGCCCCAAACCTCCTTCTCACTTGTGATCGCCCAGACCTGGATCTCCGCTTC
  ACCATCAAGTTCCAGGAGTATAGCCCTAATCTCTGGGGCCACGAGTTCCGCTCGCACCAC
  GATTACTACATCATTGCCCACATCGGATGGGACCCCGGGGAGGGCCTGGAAAGCCTGCCA
  GGNAAGTGGTGTGCCTACCAGAGGCATGAAAGTGCTTNCTNCGATGGGGACAAATCCCCC
  G
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001406 unedited
  AGCTATGGACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTGGGTT
  TTGGTGTTCAAATTTCCCTTTTACAGTAAAACTACTGAGGTGACGGCATATCCCGCCACC
  ATGGCAATATCAACTTCCTGTTCCCCAGAAGGAGCGATTAGAAAAATCAGGAAGGAGCTG
  GGAACTACAGCACCAGAGAGGTGAACTTGACCTCTGGTGGACAAAGCAACCATCTTGGCT
  GGTGATTGAGGTGGCCAACCTGGTGCCCAACAACAGCTTTGATCTAATAAAACTGAGCTG
  GGAGGAAACTGAGGCAACACCCAATTGCACACTAGAGGGCAGGAAGAGGTATCAGCTGGT
  CACTATGGAAACAGTGGCCTTTGCTAAGTACCACTGTCAGCTAGACCAGCCAGTAGGCCA
  CCATTTTGGTCACTAAGAAGGGAACTGTGTTGAGTCTGAAAGGAGTCAGAAATGTAGGAT
  GGAATGAACAAGACAGCCATCTGGGTTACTCGGGAGAGCACATGGGTTCTTGGGGTAGTC
  CTGTTGATTAACAGAGATTATACTGGGATATGGAAAGGAAAGAACAAGACAAATTANGCC
  TGCCACAGCACATGGCAGTCATCTTAGCTGTCCTAAAATCAGCACAGAGCACAAGGGAAG
  GGAGATCTTGGGAAGTGCCACTGAAGACTGGGAAAAGTCCTTCTCGGTCCACCTCTAACC
  TCTTACAGTCAGTAAGTTCCAGCTTCCTCCTGAAGGAAAGTCTTCCCGCCTTCTTCTCCC
  AGAATATGAACATCCACCTTCACTTCTGATTCCCAGACAAGCCATTTGGATTCCAGCCAG
  ACCACTTCTGTTTCAGAGTGCTACCTTCTGTCTGGATGAAGGACTTCCAGTTCTGCTGAC
  TGTTCTGGCATTCCCAGCCATTTTGNTGGCCCTGTCACCTCTGTCTGAAGAGTCATTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001406
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001406.3, NP_001397.1
• RefSeq Size: 3236 bp
• RefSeq ORF: 1023 bp
• Locus ID: 1949
• Cytogenetics: 17p13.1
• Domains: Ephrin
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: ' EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]'