EIF4B (NM_001417) Human Untagged Clone

CAT#: SC119269

EIF4B (untagged)-Human eukaryotic translation initiation factor 4B (EIF4B)


  "NM_001417" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EIF4B
Synonyms EIF-4B; PRO1843
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119269 sequence for NM_001417 edited (data generated by NextGen Sequencing)
ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGACTTT
CTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGCTGG
GCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGATGAC
GATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCTGCT
CGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTTCTA
GGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTAAAT
ATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTTGGT
TATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAGTCT
CTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGGGAT
GATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGGAGG
GCTCGTCCTGCTACAGACAGCTTTGATGACTACCCACCTAGAAGAGGTGATGATAGCTTT
GGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGGATGGG
TATCGGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCGGGATCGCTATGATGAC
CGAGGCAGCAGAGACTATGATAGAGGCTATGATTCCCGGATAGGCAGTGGCAGAAGAGCA
TTTGGCAGTGGGTATCGCAGGGATGATGACTACAGAGGAGGCGGGGACCGCTATGAAGAC
CGATATGACAGACGGGATGATCGGTCGTGGAGCTCCAGAGATGATTACTCTCGGGATGAT
TATAGGCGTGATGATAGAGGTCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGT
ACTCCTAAGGAAGATGATTCCTCTGCTAGTACCTCCCAGTCCACTCGAGCTGCTTCTATC
TTTGGAGGGGCAAAGCCTGTTGACACAGCTGCTAGAGAAAGAGAAGTAGAAGAACGGCTA
CAGAAGGAACAAGAGAAGTTGCAGCGTCAGCTGGATGAGCCAAAACTAGAACGACGGCCT
CGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAACGGGAACGGTCGAGGACA
GGAAGTGAGTCATCACAAACTGGGACCTCCACCACATCTAGCAGAAATGCACGAAGGAGA
GAGAGTGAGAAGTCTCTAGAAAATGAAACACTCAATAAGGAGGAAGATTGCCACTCTCCA
ACTTCTAAACCTCCCAAACCTGATCAGCCCCTAAAGGTAATGCCAGCCCCTCCACCAAAG
GAGAATGCTTGGGTGAAGCGAAGTTCTAACCCTCCTGCTCGATCTCAGAGCTCAGACACA
GAGCAGCAGTCCCCTACAAGTGGTGGGGGAAAAGTAGCTCCAGCTCAACCATCTGAGGAA
GGACCAGGAAGGAAAGATGAAAATAAAGTAGATGGGATGAATGCCCCAAAAGGCCAAACT
GGGAACTCTAGCCGTGGTCCAGGAGACGGAGGGAACAGAGACCACTGGAAGGAGTCAGAT
AGGAAAGATGGCAAAAAGGATCAAGACTCCAGATCTGCACCTGAGCCAAAGAAACCTGAG
GAAAATCCAGCTTCYAAGTTCAGTTCTGCAAGCAAGTATGCTGCTCTCTCTGTTGATGGT
GAAGATGAAAATGAGGGAGAAGATTATGCCGAATAG

Clone variation with respect to NM_001417.4
1755 c=>y
>OriGene 5' read for NM_001417 unedited
NGGCGTGTCATATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCTCCC
AACATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGAC
TTTCTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGC
TGGGCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGAT
GACGATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCT
GCTCGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTT
CTAGGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTA
AATATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTT
GGTTATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAG
TCTCTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGG
GATGATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGG
AGGGCTCGTCCTGCTACAGACAGCTNTGATGACTACCCACCTAGAAGAGGTGATGATAGC
TNTGGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGNAT
GGGGTATCGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCCGGNATCGCTATGA
TGACCCGAGCAGCAGAGACTATGATAGAGGCTATGATTCCCGNATAGCAGTGGCAGAAGA
GCATTTGNCA
>OriGene 3' read for NM_001417 unedited
CTATGTACGCGGCCGCAATCTATAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTAAAGAAAAGGGGGATTTTTATTTTGTCTTGTCGGAATA
AAGGTTTTGATTTGCTCTCAAATGTCCCAGGGGGGAAAAAACCTAGGAAAAAAACCCCAG
GATGTAAAGGCCTTTTCGGCATAATCTTCCCCCTCTTTTTCTTCTTCCCCATCAACAAAA
AAACCACCTTCCTTGCTTGCAAAACTGAACTTGAAAGCTGGATTTTCCTCAGGTTTTTTT
GGCTCAGGGGCAAATCGGGAGTCTTGACCCTTTTTGCCATCTTTCCTATCTGACTCCTTC
CAGGGGTCTTTGTCCCCTCCGTCTCCTGAACCACGGTTAAATTTCCCATTTGGGCCTTTG
GGGGCTTTCACCCCATCAACTTTATTTTCATCTTTCCTTCCGGGCCCTTCCTAAAATGGG
GGAGCGGGACCTACTTTTCCCCCACCACTTGTAGGGAACTGCTGCTCTGGGTCTGACCTC
TGAAATCAACCAGGAGGGTTAAAACCTTCCTTTCCCCAACCATTCTCCTTTGGGGGAGGG
GCGGGCTTTACCTTTAGGGGCTGACCAGGTTGGGAAGGTCTAAAATTCGGAAATGGGCAT
CCTCCCCCTTTTGGAGGGTTAATTTCCTAGAAACTTTCCCTCCTTTCCTCTTCGGCTTTC
TGCAAATGGGGGGGAGGCCCCCGTCTGGGATACCCCCTACTTGTCCCAACCGCCCCGTAC
TCGATTTCTTCCCTACACCATTTGCGTGTACACTCCACGTCCTCTCTTACTACCTCTCTC
CCCCTCTCCACGCTCTCTTCTCACTTCCTCACCCTCTCACCACCCTCTTTGCCCCATCCT
TTCTTTTCTTATCCCCACCCCCCCTCCCTATCTCTCTTTCACCCACACCACCCCACTTCC
TCTCATCCTCTCATACCTCTT
Restriction Sites NotI-NotI     
ACCN NM_001417
Insert Size 2090 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001417.2, NP_001408.2
RefSeq Size 3919 bp
RefSeq ORF 1836 bp
Locus ID 1975
Cytogenetics 12q13.13
Domains RRM
Protein Pathways mTOR signaling pathway
Gene Summary ''
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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