DAXX (NM_001350) Human Untagged Clone

CAT#: SC119273

DAXX (untagged)-Human death-domain associated protein (DAXX), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAXX
• Synonyms: BING2; DAP6; EAP1; SMIM40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119273 sequence for NM_001350 edited (data generated by NextGen Sequencing)
  ATGGCCACCGCTAACAGCATCATCGTGCTGGATGATGATGACGAAGATGAAGCAGCTGCT
  CAGCCAGGGCCCTCCCACCCACTCCCCAATGCGGCCTCACCTGGGGCAGAAGCCCCTAGC
  TCCTCTGAGCCTCATGGGGCCAGAGGAAGCAGTAGTTCGGGCGGCAAGAAATGCTACAAG
  CTGGAGAATGAGAAGCTGTTCGAAGAGTTCCTTGAACTTTGTAAGATGCAGACAGCAGAC
  CACCCTGAGGTGGTCCCATTCCTCTATAACCGGCAGCAACGTGCCCACTCTCTGTTTTTG
  GCCTCGGCGGAGTTCTGCAACATCCTCTCTAGGGTCCTGTCTCGGGCCCGGAGCCGGCCA
  GCCAAGCTCTATGTCTACATCAATGAGCTCTGCACTGTTCTCAAGGCCCACTCAGCCAAA
  AAGAAGCTGAACTTGGCCCCTGCCGCCACCACCTCCAATGAGCCCTCTGGGAATAACCCT
  CCCACACACCTCTCCTTGGACCCCACAAATGCTGAAAACACTGCCTCTCAGTCTCCAAGG
  ACCCGTGGTTCCCGGCGGCAGATCCAGCGTTTGGAGCAGCTGCTGGCGCTCTATGTGGCA
  GAGATCCGGCGGCTGCAGGAAAAGGAGTTGGATCTCTCAGAATTGGATGACCCAGACTCC
  GCATACCTGCAGGAGGCACGGTTGAAGCGTAAGCTGATCCGCCTCTTTGGGCGACTATGT
  GAGCTGAAAGACTGCTCTTCACTGACCGGCCGTGTCATAGAGCAGCGCATCCCCTACCGT
  GGCACCCGCTACCCAGAGGTTAACAGGCGCATTGAGCGGCTCATCAACAAGCCAGGGCCT
  GATACCTTCCCTGACTATGGGGATGTGCTTCGGGCTGTAGAGAAGGCAGCTGCCCGACAC
  AGCCTTGGCCTCCCCCGACAGCAGCTCCAGCTCATGGCTCAGGATGCCTTCCGAGATGTG
  GGCATCAGGTTACAGGAGCGACGTCACCTCGATCTCATCTACAACTTTGGCTGCCACCTC
  ACAGATGACTATAGGCCAGGCGTTGACCCTGCACTATCAGATCCTGTGTTGGCCCGGCGC
  CTTCGGGAAAACCGGAGTTTGGCCATGAGTCGGCTGGATGAGGTCATCTCCAAATATGCA
  ATGTTGCAAGACAAAAGTGAGGAGGGCGAGAGAAAAAAGAGAAGAGCTCGGCTCCAAGGC
  ACCTCTTCCCACTCTGCAGACACCCCCGAAGCCTCCTTGGATTCTGGTGAGGGCCCTAGT
  GGAATGGCATCCCAGGGGTGCCCTTCTGCCTCCAGAGCTGAGACAGATGACGAAGACGAT
  GAGGAGAGTGATGAGGAAGAGGAGGAGGAGGAGGAAGAAGAAGAGGAGGAGGCCACAGAT
  TCTGAAGAGGAGGAGGATCTGGAACAGATGCAGGAGGGTCAGGAGGATGATGAAGAGGAG
  GACGAAGAGGAAGAAGCAGCAGCAGGTAAAGATGGAGACAAGAGCCCCATGTCCTCACTA
  CAGATCTCCAATGAAAAGAACCTGGAACCTGGCAAACAGATCAGCAGATCTTCAGGGGAG
  CAGCAAAACAAAGGACGCATAGTGTCACCATCGTTACTGTCAGAAGAACCCCTGGCCCCC
  TCCAGCATAGATGCTGAAAGCAATGGAGAACAGCCTGAGGAGCTGACCCTGGAGGAAGAA
  AGCCCTGTGTCTCAGCTCTTTGAGCTAGAGATTGAAGCTTTGCCCCTGGATACCCCTTCC
  TCTGTGGAGACGGACATTTCCTCTTCCAGGAAGCAATCAGAGGAGCCCTTCACCACTGTC
  TTAGAGAATGGAGCAGGCATGGTCTCTTCTACTTCCTTCAATGGAGGCGTCTCTCCTCAC
  AACTGGGGAGATTCTGGTCCCCCCTGCAAAAAATCTCGGAAGGAGAAGAAGCAAACAGGA
  TCAGGGCCATTAGGAAACAGCTATGTGGAAAGGCAAAGGTCAGTGCATGAGAAGAATGGG
  AAAAAGATATGTACCCTGCCCAGCCCACCTTCCCCCTTGGCTTCCTTGGCCCCAGTTGCT
  GATTCCTCCACGAGGGTGGACTCTCCCAGCCATGGCCTGGTGACCAGCTCCCTCTGCATC
  CCTTCTCCAGCCCGGCTGTCCCAAACCCCCCATTCACAGCCTCCTCGGCCTGGTACTTGC
  AAGACAAGTGTGGCCACACAATGCGATCCAGAAGAGATCATCGTGCTCTCAGACTCTGAT
  TAG
  
  Clone variation with respect to NM_001350.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001350 unedited
  GGTTTANAATAGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTGGGAGTG
  GGAAGGAAGGCGGAGGGAACCATGCGAGGTTCTGAGAATTGCGGCGAGGGTCGCCTCGAG
  AGACGGTTTCTGAGCAGGAATTCTGAAATCCCCACCACTTCCTCCCTCCGGGGGATTTGA
  TCCCCTATGGCCACCGCTAACAGCATCATCGTGCTGGATGATGATGACGAAGATGAAGCA
  GCTGCTCAGCCAGGGCCCTCCCACCCACTCCCCAATGCGGCCTCACCTGGGGCAGAAGCC
  CCTAGCTCCTCTGAGCCTCATGGGGCCAGAGGAAGCAGTAGTTCGGGCGGCAAGAAATGC
  TACAAGCTGGAGAATGAGAAGCTGTTCGAAGAGTTCCTTGAACTTTGTAAGATGCAGACA
  GCAGACCACCCTGAGGTGGTCCCATTCCTCTATAACCGGCAGCAACGTGCCCACTCTCTG
  TTTTTGGCCTCGGCGGAGTTCTGCAACATCCTCTCTAGGGTCCTGTCTCGGGCCCGGAGC
  CGGCCAGCCAAGCTCTATGTCTACATCAATGAGCTCTGCACTGTTCTCAAGGCCCACTCA
  GCCAAAAAGAAGCTGAACTTGGCCCCTGCCGCCACCACCTCCAATGAGCCCTCTGGGAAT
  AACCCTCCCACACACCTCTCCTTGGACCCCACAAATGCTGAAAACACTGCCTCTCAGTCT
  CCAAGGACCCGTGGTTCCCGGCGGCAGATTCAGCGTTTGGAGCAGCTGCTGGCGCTCTAT
  GGGGCAGAGATCCGGCGGCTGCAGGACAAGGAGTTGGATCTCTCAAANTTGGATGACCCA
  GACTCCGCATACCTGCAGGAGGCACGGCTGAAGCGTAAGCTGATCCGGCTCTTGGGCGAC
  TATGNGAGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001350 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAGAAACACCACCAAAGGGGATT
  AGCTTAGTCCATCCCTTCCTCAGTCATCTGCTTCCCACCTTCCTCCAAATGTTATCCCAT
  AACATTCTGGAGGCAGGGAGAAGGGGAGGCAGCTAATCAGAGTCTGAGAGCACGATGATC
  TCTTCTGGATCGCATTGTGTGGCCACACTTGTCTTGCAAGTACCAGGCCGAGGAGGCTGT
  GAATGGGGGGTTTGGGACAGCCGGGCTGGAGAAGGGATGCAGAGGGAGCTGGTCACCAGG
  CCATGGCTGGGAGAGTCCACCCTCGTGGAGGAATCAGCAACTGGGGCCAAGGAAGCCAAG
  GGGGAAGGTGGGCTGGGCAGGGTACATATCTTTTTCCCATTCTTCTCATGCACTGACCTT
  TGCCTTTCACATAGCTGTTTCCTAATGGCCCTGATCCTGTTTGCTTCTTCTCCTTCCGAG
  ATTTTTTGCAGGGGGGACCAGAATCTCCCCAGTTGTGAGGAGAGACGCCTCCCTTGAAGG
  AAGTAGAAGAGACCATGCCTGCTCCATTCTCTAAGACAGCGGTGAAGGGCTCCTCTGATT
  GCTTCCTGGAAAGGAATTGTCCGCCTCCACAGAGAAGGGGTATCCAAGGGCAAAGCTTCA
  ATCTCTAGCTCAAAGAGCCGAGACACAGGCCTTTCCTTCCTACCAGGAANCCTCCTCAAG
  CCGGTCTCCCATTGCTTTCACCTTCTATGCTGGAGGGGCCCACGGTTTCTCTGACAATAA
  CCAGGGGGACCTATGCGCCCCTTTGTCTTGCTGTTCCCCCGAAAACCGCTGTCTGTCTGC
  CCCGTTCACGTCTTTTTTTGGAAATTCGCCTCAGACTGGGGCTCTTCCCCCCTTTTTCCA
  CCGCCGGTCCTTCCTTTCGTCTCCTTTCTATCTCTTCTATCTCTACTTTGTTCCCAATCC
  CACTTTAAAACAGGGGCCTCCCTTTTTCTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001350
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001350.3, NP_001341.1
• RefSeq Size: 2477 bp
• RefSeq ORF: 2223 bp
• Locus ID: 1616
• Cytogenetics: 6p21.32
• Domains: Daxx
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway
• Gene Summary: 'This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a).