DDX3 (DDX3X) (NM_001356) Human Untagged Clone

CAT#: SC119279

DDX3X (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX3X
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2; MRX102
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119279 sequence for NM_001356 edited (data generated by NextGen Sequencing)
  ATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCAGTTTGCTGGCCTAGAC
  CTGAACTCTTCAGATAATCAGAGTGGAGGAAGTACAGCCAGCAAAGGGCGCTATATTCCT
  CCTCATTTAAGGAACCGAGAAGCTACTAAAGGTTTCTACGATAAAGACAGTTCAGGGTGG
  AGTTCTAGCAAAGATAAGGATGCGTATAGCAGTTTTGGATCTCGTAGTGATTCAAGAGGG
  AAGTCTAGCTTCTTCAGTGATCGTGGAAGTGGATCAAGGGGAAGGTTTGATGATCGTGGA
  CGGAGTGATTACGATGGCATTGGCAGCCGTGGTGACAGAAGTGGCTTTGGCAAATTTGAA
  CGTGGTGGAAACAGTCGCTGGTGTGACAAATCAGATGAAGATGATTGGTCAAAACCACTC
  CCACCAAGTGAACGCTTGGAACAGGAACTCTTTTCTGGAGGCAACACTGGGATTAATTTT
  GAGAAATACGATGACATTCCAGTTGAGGCAACAGGCAACAACTGTCCTCCACATATTGAA
  AGTTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTGAGCTTACTCGTTAT
  ACTCGCCCAACTCCAGTGCAAAAGCATGCTATTCCTATTATCAAAGAGAAAAGAGACTTG
  ATGGCTTGTGCCCAAACAGGGTCTGGAAAAACTGCAGCATTTCTGTTGCCCATCTTGAGT
  CAGATTTATTCAGATGGTCCAGGCGAGGCTTTGAGGGCCATGAAGGAAAATGGAAGGTAT
  GGGCGCCGCAAACAATACCCAATCTCCTTGGTATTAGCACCAACGAGAGAGTTGGCAGTA
  CAGATCTACGAGGAAGCCAGAAAATTTTCATACCGATCTAGAGTTCGTCCTTGCGTGGTT
  TATGGTGGTGCCGATATTGGTCAGCAGATTCGAGACTTGGAACGTGGATGCCATTTGTTA
  GTAGCCACTCCAGGACGTCTAGTGGATATGATGGAAAGAGGAAAGATTGGATTAGACTTT
  TGCAAATACTTGGTGTTAGATGAAGCTGATCGGATGTTGGATATGGGGTTTGAGCCTCAG
  ATTCGTAGAATAGTCGAACAAGATACTATGCCTCCAAAGGGTGTCCGCCACACTATGATG
  TTTAGTGCTACTTTTCCTAAGGAAATACAGATGCTGGCTCGTGATTTCTTAGATGAATAT
  ATCTTCTTGGCTGTAGGAAGAGTTGGCTCTACCTCTGAAAACATCACACAGAAAGTAGTT
  TGGGTGGAAGAATCAGACAAACGGTCATTTCTGCTTGACCTCCTAAATGCAACAGGCAAG
  GATTCACTGACCTTAGTGTTTGTGGAGACCAAAAAGGGTGCAGATTCTCTGGAGGATTTC
  TTATACCATGAAGGATACGCATGTACCAGCATCCATGGAGACCGTTCTCAGAGGGATAGA
  GAAGAGGCCCTTCACCAGTTCCGCTCAGGAAAAAGCCCAATTTTAGTGGCTACAGCAGTA
  GCAGCAAGAGGACTGGACATTTCAAATGTGAAACATGTTATCAATTTTGACTTGCCAAGT
  GATATTGAAGAATATGTACATCGTATTGGTCGTACGGGACGTGTAGGAAACCTTGGCCTG
  GCAACCTCATTCTTTAACGAGAGGAACATAAATATTACTAAGGATTTGTTGGATCTTCTT
  GTTGAAGCTAAACAAGAAGTGCCGTCTTGGTTAGAAAACATGGCTTATGAACACCACTAC
  AAGGGTAGCAGTCGTGGACGTTCTAAGAGTAGCAGATTTAGTGGAGGGTTTGGTGCCAGA
  GACTACCGACAAAGTAGCGGTGCCAGCAGTTCCAGCTTCAGCAGCAGCCGCGCAAGCAGC
  AGCCGCAGTGGCGGAGGTGGCCACGGTAGCAGCAGAGGATTTGGTGGAGGTGGCTATGGA
  GGCTTTTACAACAGTGATGGATATGGAGGAAATTATAACTCCCAGGGGGTTGACTGGTGG
  GGTAACTGA
  
  Clone variation with respect to NM_001356.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001356 unedited
  GGGATATTTGAAAACCACGCACTTTCACTATAGGGCGGCACGCGCAATTCGGCACGAGGC
  GTGAGAGGGCCTTCGCGGTGGAACAAACACTCGCTTAGCAGCGGAAGACTCCGAGTTCTC
  GGTACTCTTCAGGGATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCAGT
  TTGCTGGCCTAGACCTGAACTCTTCAGATAATCAGAGTGGAGGAATTACAGCCAGCAAAG
  GGCGCTATATTCCTCCTCATTTAAGGAACCGAGAAGCTACTAAAGGTTTCTACGATAAAG
  ACAGTTCAGGGTGGAGTTCTAGCAAAGATAAGGATGCGTATAGCAGTTTTGGATCTCGTA
  GTGATTCAAGAGGGAAGTCTAGCTTCTTCAGTGATCGTGGAAGTGGATCAAGGGGAAGGT
  TTGATGATCGTGGACGGAGTGATTACGATGGCATTGGCAGCCGTGGTGACAGAAGTGGCT
  TTGGCAAATTTGAACGTGGTGGAAACAGTCGCTGGTGTGACAAATCAGATGAAGATGATT
  GGTCAAAACCACTCCCACCAAGTGAACGCTTGGAACAGGAACTCTTTTCTGGAGGCAACA
  CTGGGATTAATTTTGAGAAATACGATGACATTCCAGTTGAGGCAACAGGCAACAACTGTC
  CTCCACATATTGAAAGTTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTG
  AGCTTACTCGTTATACTCGCCCAACTCCAGTGCAAAAGCATGCTATTCCTATTATCAAAG
  AGAAAAGAGACTTGATGGCTTGTGCCCAAACAGGGTCTGGAAAAACTGCAGCATTTCTGG
  TGCCCATCTTGAGTCAGATTTATTCAGATGGTCCAGGCGAGGCTTTGAGGCCATGAANGA
  NATGNANGTATGGNCGCGCAACATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001356 unedited
  CTGGGCATGGCATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGCAG
  CCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTT
  TTTTTTTTTTTTTTCAGGCAAGAGCACTTTATTTACACCTCTGAAAATAAACCACAATTG
  ACAACAACAAAGGAGACTGTGCAGTTCAACTTTTTATTTTAATAAAATCAGAATATGCAC
  AGCACATGCAGTGCTAGCATCTAAACTAATACAATAAGCAATTACTAAAGCTACAGTGAC
  TTGAGGTTCAATCTTTACATTCAGCAAGTTTAAATCCTTACATGATTTTTGAAACCTTAT
  TAACTGAAAATATTGCTTGCTGATAAAACTTGCTCAAATGCTATTGCTGAATGTACAAGT
  CACTGATTATGCCAATACAACAAAGATAACCACATGTTTGAGGATTGCAATGTGCCAGAC
  ATTCACTAAAAAAAAAAAAAAAACACACAACTAAACAAAATCCACACAACAAACATCTGA
  GAATCTGGACACTTCACATCAGTGTTTTGAAGTGTTTCATTAATATCTTAAGACAAGTAT
  ATCAAAACCTTGGCATGATTTAATTTCAAGTCGCCAATTTTGTTTTTACTAACATCAAAA
  AGTTATGGCTACACTGCCAACGCCGGGTCTGCTTAATTTGACTTAAGAATTTAATATGAC
  TTAAACATTAAAAGCTCCACTCCCCCGAAATATAAAATTTCCCGCGAACGGGGAACATTA
  CACATTCAAGTGCCCAGTGTTTTTGAACCTGGCTTTTGGGGCAAGTTTTCCTTAAAATTT
  CCAAGAAAGAACGTTTAGGCTTAAAAAATAAAAATTGGCTCAGAGGTCGCAAAATTTAGC
  AAAACAACCGGCCAAAGTGTCTGGAGACCTGCGGGCGAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001356
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001356.2, NP_001347.2
• RefSeq Size: 5322 bp
• RefSeq ORF: 1989 bp
• Locus ID: 1654
• Cytogenetics: Xp11.4
• Domains: DEAD, helicase_C
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).