DKC1 (NM_001363) Human Untagged Clone

CAT#: SC119282

DKC1 (untagged)-Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DKC1
• Synonyms: CBF5; DKC; DKCX; NAP57; NOLA4; XAP101
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119282 sequence for NM_001363 edited (data generated by NextGen Sequencing)
  ATGGCGGATGCGGAAGTAATTATTTTGCCAAAGAAACATAAGAAGAAAAAGGAGCGGAAG
  TCATTGCCAGAAGAAGATGTAGCCGAAATACAACACGCTGAAGAATTTCTTATCAAACCT
  GAATCCAAAGTTGCTAAGTTGGACACGTCTCAGTGGCCCCTTTTGCTAAAGAATTTTGAT
  AAGCTGAATGTAAGGACAACACACTATACACCTCTTGCATGTGGTTCAAATCCTCTGAAG
  AGAGAGATTGGGGACTATATCAGGACAGGTTTCATTAATCTTGACAAGCCCTCTAACCCC
  TCTTCCCATGAGGTGGTAGCCTGGATTCGACGGATACTTCGGGTGGAGAAGACAGGGCAC
  AGTGGTACTCTGGATCCCAAGGTGACTGGTTGTTTAATCGTGTGCATAGAACGAGCCACT
  CGCTTGGTGAAGTCACAACAGAGTGCAGGCAAAGAGTATGTGGGGATTGTCCGGCTGCAC
  AATGCTATTGAAGGGGGGACCCAGCTTTCTAGGGCCCTAGAAACTCTGACAGGTGCCTTA
  TTCCAGCGACCCCCACTTATTGCTGCAGTAAAGAGGCAGCTCCGAGTGAGGACCATCTAC
  GAGAGCAAAATGATTGAATACGATCCTGAAAGAAGATTAGGAATCTTTTGGGTGAGTTGT
  GAGGCTGGCACCTACATTCGGACATTATGTGTGCACCTTGGTTTGTTATTGGGAGTTGGT
  GGTCAGATGCAGGAGCTTCGGAGGGTTCGTTCTGGAGTCATGAGTGAAAAGGACCACATG
  GTGACAATGCATGATGTGCTTGATGCTCAGTGGCTGTATGATAACCACAAGGATGAGAGT
  TACCTGCGGCGAGTTGTTTACCCTTTGGAAAAGCTGTTGACATCTCATAAACGGCTGGTT
  ATGAAAGACAGTGCAGTAAATGCCATCTGCTATGGGGCCAAGATTATGCTTCCAGGTGTT
  CTTCGATATGAGGACGGCATTGAGGTCAATCAGGAGATTGTGGTTATCACCACCAAAGGA
  GAAGCAATCTGCATGGCTATTGCATTAATGACCACAGCGGTCATCTCTACCTGCGACCAT
  GGTATAGTAGCCAAGATCAAGAGAGTGATCATGGAGAGAGACACTTACCCTCGGAAGTGG
  GGTTTAGGTCCAAAGGCAAGTCAGAAGAAGCTGATGATCAAGCAGGGCCTTCTGGACAAG
  CATGGGAAGCCCACAGACAGCACACCTGCCACCTGGAAGCAGGAGTATGTTGACTACAGT
  GAGTCTGCCAAAAAAGAGGTGGTTGCTGAAGTGGTAAAAGCCCCGCAGGTAGTTGCCGAA
  GCAGCAAAAACTGCGAAGCGGAAGCGAGAGAGTGAGAGTGAAAGTGACGAGACTCCTCCA
  GCAGCTCCTCAGTTGATCAAGAAGGAAAAGAAGAAGAGTAAGAAGGACAAGAAGGCCAAA
  GCTGGTCTGGAGAGCGGGGCCGAGCCTGGAGATGGGGACAGTGATACCACCAAGAAGAAG
  AAGAAGAAGAAGAAAGCAAAAGAGGTAGAATTGGTTTCTGAGTAG
  
  Clone variation with respect to NM_001363.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001363 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTGTGGACCGGGCGGC
  ACGCACGCGGTGCAGGGTAACATGGCGGATGCGGAAGTAATTATTTTGCCAAAGAAACAT
  AAGAAGAAAAAGGAGCGGAAGTCATTGCCAGAAGAAGATGTAGCCGAAATACAACACGCT
  GAAGAATTTCTTATCAAACCTGAATCCAAAGTTGCTAAGTTGGACACGTCTCAGTGGCCC
  CTTTTGCTAAAGAATTTTGATAAGCTGAATGTAAGGACAACACACTATACACCTCTTGCA
  TGTGGTTCAAATCCTCTGAAGAGAGAGATTGGGGACTATATCAGGACAGGTTTCATTAAT
  CTTGACAAGCCCTCTAACCCCTCTTCCCATGAGGTGGTAGCCTGGATTCGACGGATACTT
  CGGGTGGAGAAGACAGGGCACAGTGGTACTCTGGATCCCAAGGTGACTGGTTGTTTAATC
  GTGTGCATAGAACGAGCCACTCGCTTGGTGAAGTCACAACAGAGTGCAGCAAAGAGTATG
  TGGGGATTGTCCGGCTGCACAATGCTATTGAAGGGGGGACCCAGCTTTCTAGGGCCCTAG
  AAACTCTGACAGGTGCCTTATTCCAGCGACCCCCACTTATTGCTGCAGTAAAGAGGCAGC
  TCCGAGTGAGGACCATCTACGAGAGCAAATGATTGAATACGATCCTGAAAGAAATTANGA
  ATCTTTTGGNGGTGAGTGTGAGGCTGGCACCTCATTCGACATTATGTGTGCACCTTGGTT
  NGTATTGGAAANTTGTGGGCAATGCAGACTTTNNNAGGGGGTCGTTCTGAGTCATAGTGA
  AAGGACACATGTGACAGCCTGAGTGCTGAGGCTATGGCTGATGAACACAGGAGAAAGTCT
  GGGGCGATTGTTACTTGGAAAGCGTGATTCTAACGCGGTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001363 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTCAAATAGGACATA
  AAGGTCCCTAAATTTTTAATCAAAATATTTTACAATTTTTGCTCTGCTAGCATGAGATCA
  ACAAGAAAACCCAACAGGAATACATAAAAAACTTAACAAGTTGACTTTAATAAGTAAAAA
  TTACAACATTCTTCTCCCAGCAATGAACAATTTCATTTTTTAAAAGTAAACGAGGCCTAA
  AACAAGAGGGCACAGGAACAAGTAGTCAGATGGATTTAGGTGAGCACTGTACACAAGCTT
  TGAGGAAGTGCAAAGGACTGACCTCTAGGCCAGAACAAGATGGAAAACTACCAGGCCCAT
  CGGGCCTATAACCCAGACACCAGCATGGACAAAACTCAGTTATACTGAATTCAGAGACAA
  AATTCAGTGACACTCTTCTACCACTTATTTAGGGTTCTACAGCATTTCACTGAGCAGACT
  TAGTTTTTTGTTTTTGTTTTACAAACCTTTTAAACAGAGGGGATTTAAAAAATGCAAACT
  AGGACTACGATGTTAAGACAACCACTAGCAGACAGCTGCGGACAGTTACTGGGTCTGAAG
  GTGAGGCTTCCCAACTCAAACAGAGAAGTCATTGGGATAAACTCTGCCTCTTTCATCTTT
  CACCACCCATATCCTTAAAACAGGACAAGATGGGATGACCACACCAGGTAACATCACCGA
  GGTCTCAAAGTAGCAGCTAAAATGTGCCAGAACTCTTCACCCTGTCTAGGACCTATGTTC
  CACTCTCTCAGCAACAAAAGGATCTATAACATGGGTTTCTCAATAAGGGCTTTAGTTTCT
  CCTCCAGCTTCAAGTGGCCTTCACTACTCAGAACCCNANTNCTACCTCTTNGGGCTTCCT
  CTCTTCTTCTTCTCTTGGGGGTATCACTGGNCCCATTTCCAGGCTCGGGCCCGCTCTCAA
  AACAGATTTGCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001363
• Insert Size: 2450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001363.2, NP_001354.1
• RefSeq Size: 2465 bp
• RefSeq ORF: 1545 bp
• Locus ID: 1736
• Cytogenetics: Xq28
• Domains: PUA, TruB_N
• Protein Families: Druggable Genome
• Gene Summary: 'This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).