Cleavage stimulation factor 2 (CSTF2) (NM_001325) Human Untagged Clone

CAT#: SC119306

CSTF2 (untagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSTF2
• Synonyms: CstF-64
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119306 sequence for NM_001325 edited (data generated by NextGen Sequencing)
  ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
  GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
  CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
  TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
  GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
  CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
  CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
  TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
  ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
  ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
  ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
  ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
  GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
  CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
  CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
  GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
  TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
  GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
  GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
  AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
  GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
  GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
  GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
  GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
  GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
  GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
  GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
  ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
  CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTGA
  
  Clone variation with respect to NM_001325.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001325 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGCCGACTCAACAG
  AGCTATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTT
  CGTGGGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGT
  TGGACCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTA
  TGGCTTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGG
  GCGCGAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGA
  AGAGCTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCAT
  CAGTCCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCA
  GATGTTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACG
  GAACATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGAT
  GAGAATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAAC
  GCTGATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCANGATCCCATGT
  GTCAATGAACCAGCAGAATCCTTCAGGCCCCTCAGCCCAGTCTTTGGGNTGGAATGCATG
  TCATGGGCGCACCTNCTCTGNATGCAGCTTTATGCAGGGGTGGAGNTCCAGCACAGGGCA
  ATGGCAGCTGCTGTACAGGACCTGGNCCCTGGTNCTTTACTNCTGNNAGAGAAATGCAGC
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001325 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGTTCAAAGTTTATTTCAAGTCACAG
  AAAATATACAAACTAAGACAATAAAATGAAGTGATTTTGTTCTGAGAGAAGGTGATGGGA
  GTCCTCCACCTGGCACTGATTTGAGTCATTCAAGGGTTAAGATGCAAGTCATCTTTTTTC
  AATATTTCAACAAAATTATAGAATTTCCAGATTTCTTGCCAGGTATTTTTGAAAACCTAT
  CAAGGTGCTCCAGTGGATTTCTGTATTTGTTCCTTTAAAATCAAGATACTCTGCCTTTGC
  TCAAGAGGCAACATGGCAATCTGGTCTGCAGTCAGTTGTAGAACCTGCATAATCAAAGCA
  GCCTTCTCATGATCCTGTGGAGTGACTTGGTTCTGCCCGGGACTAAAGCCGCCAGGCTGG
  CTTCCACCCTGTATACTTGCTCCTTGCATTCCTGTTCCCTGCATGACTGGGACCTGTCTG
  GATCCCTGGGGGACAACCGCCCCCATGTTAATTGGACTGGGACCCTGCATTCCACTAAGT
  ATAGGTCCTCTGGGGCCAGGCACTGAGCCTCTGGTATCCATGCCTCTGGCCTCCATCCCT
  CGGACTTNCATTGCACGGGCCTCCATCGCTCGGGCCTCCATTGCACGAGCTTCCATCGCA
  CGGGCCTCCATAGCACGGGCCTCTAATCCTCTGGCATCTACCCTCTTGCCTCCATGGCTC
  GGGCCTCCTCCCTCGTGCATCTATTCCTCGGGGATCCTTCCACCTCTGCCATCCAGGTTG
  GACCCCTCTGATCANCATGGTCCTCCTGGTCCTGCATANAAGGCTGGGCTCGGTATGACC
  CTCCCTCATTCATGTGGGGAGGCCCTGGCTCTCATGCCAGGACATGGGCATGGTGGACCT
  GATGAGGGGGCCCAGTAACCCTAGGCTTACTCCTCGTACCAAAGAAAGGGCTCCCCGGAA
  TATCCGGACATTTCTACAACCCGAGGGTTGAACTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001325
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001325.1, NP_001316.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 1734 bp
• Locus ID: 1478
• Cytogenetics: Xq22.1
• Domains: RRM
• Gene Summary: 'This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.