CSTF3 (NM_001326) Human Untagged Clone

CAT#: SC119307

CSTF3 (untagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa (CSTF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSTF3
• Synonyms: CSTF-77
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119307 sequence for NM_001326 edited (data generated by NextGen Sequencing)
  ATGTCAGGAGACGGAGCCACGGAGCAGGCAGCTGAGTATGTCCCAGAGAAGGTGAAGAAA
  GCGGAAAAGAAATTAGAAGAGAATCCATATGACCTTGATGCTTGGAGCATTCTCATTCGA
  GAGGCACAGAATCAACCTATAGACAAAGCACGGAAGACTTATGAACGCCTTGTTGCCCAG
  TTCCCCAGTTCTGGCAGATTCTGGAAACTGTACATTGAAGCAGAGATTAAAGCTAAAAAT
  TATGACAAGGTTGAAAAGCTATTTCAGAGATGCCTTATGAAGGTTTTGCACATTGATTTA
  TGGAAGTGTTATCTTTCATATGTCCGAGAAACCAAGGGTAAACTACCAAGTTACAAAGAA
  AAAATGGCTCAAGCATATGACTTTGCACTGGATAAAATTGGAATGGAAATTATGTCCTAT
  CAGATTTGGGTGGATTACATCAATTTCCTAAAAGGCGTGGAAGCTGTAGGATCTTATGCA
  GAAAATCAAAGAATAACAGCTGTCCGAAGAGTTTATCAACGAGGTTGTGTTAATCCGATG
  ATCAACATTGAACAGCTCTGGAGAGACTATAACAAGTATGAAGAGGGTATCAATATTCAT
  TTAGCTAAAAAAATGATTGAAGATCGGAGTAGAGATTATATGAATGCTAGACGTGTAGCA
  AAGGAATATGAGACAGTAATGAAAGGCTTGGACCGTAATGCTCCCTCGGTGCCTCCTCAG
  AATACTCCTCAAGAAGCTCAACAAGTAGATATGTGGAAGAAATATATACAGTGGGAAAAG
  AGCAACCCTCTTCGTACAGAGGATCAGACCCTTATAACAAAAAGAGTTATGTTTGCTTAT
  GAACAGTGCCTGCTTGTGCTGGGCCATCACCCTGATATTTGGTATGAAGCTGCCCAGTAT
  CTTGAGCAGTCAAGTAAACTGCTCGCAGAAAAGGGAGATATGAATAATGCCAAATTATTT
  AGTGATGAAGCTGCTAATATATATGAAAGAGCCATAAGCACTTTATTGAAGAAGAATATG
  CTTTTTTATTTTGCATATGCAGATTATGAAGAGAGTCGCATGAAGTATGAAAAGGTTCAC
  AGTATATATAACAGACTTCTGGCAATTGAGGATATTGACCCTACCTTGGTATATATCCAA
  TATATGAAATTTGCACGGAGAGCAGAAGGCATCAAATCTGGAAGAATGATATTTAAAAAA
  GCAAGAGAAGATACCAGAACCCGCCACCATGTCTATGTTACTGCAGCACTCATGGAATAT
  TACTGTAGTAAGGACAAATCTGTTGCCTTTAAGATTTTTGAGCTGGGGCTAAAAAAATAT
  GGAGACATTCCAGAGTATGTCCTGGCCTATATTGACTATCTTTCTCACCTCAATGAGGAC
  AATAATACCCGAGTTTTGTTTGAACGAGTTTTAACATCTGGAAGCCTTCCTCCTGAGAAG
  TCTGGAGAAATCTGGGCCCGATTTCTAGCATTTGAAAGTAATATTGGTGATCTAGCTAGT
  ATACTCAAAGTGGAGAAAAGACGGTTTACAGCATTCAAAGAAGAGTATGAAGGGAAAGAA
  ACGGCTTTACTAGTAGATAGATACAAGTTCATGGATTTATATCCTTGCTCTGCAAGTGAA
  TTAAAAGCACTTGGTTATAAGGATGTCTCCCGTGCTAAGCTAGCAGCTATAATTCCGGAC
  CCAGTTGTAGCTCCTTCTATAGTGCCTGTTCTGAAAGATGAAGTGGATAGAAAACCAGAA
  TACCCTAAACCAGACACTCAGCAGATGATTCCATTTCAGCCACGACATTTAGCACCTCCA
  GGTTTACACCCTGTACCTGGTGGAGTGTTCCCAGTCCCTCCTGCAGCTGTTGTTTTAATG
  AAACTTCTCCCTCCTCCTATCTGTTTCCAGGGTCCTTTTGTACAAGTGGATGAACTGATG
  GAAATTTTCCGAAGATGCAAGATACCAAATACTGTTGAGGAAGCTGTGAGGATCATTACT
  GGTGGGGCCCCAGAGCTAGCTGTAGAAGGCAACGGCCCCGTGGAAAGTAATGCAGTACTC
  ACCAAGGCCGTCAAAAGGCCCAACGAGGATTCAGATGAAGATGAAGAAAAGGGAGCCGTT
  GTCCCCCCTGTTCATGACATTTACAGAGCACGGCAGCAGAAGCGGATTCGGTAG
  
  Clone variation with respect to NM_001326.2
  1024 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001326 unedited
  NGTCGGATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCGATTGGTG
  TTGGCGGTCTGGCTCAGCTGGGCAGGGGGTAACTTTACTGATTTGGGGGTGGTTTTTAGT
  TTAATTTTTCTTTTCTAGCTTCCCATCGACGGTCAGTGCGCACGTTGTAATCAGCTGAGG
  CCATGTCAGGAGACGGAGCCACGGAGCAGGCAGCTGAGTATGTCCCAGAGAAGGTGAAGA
  AAGCGGAAAAGAAATTAGAAGAGAATCCATATGACCTTGATGCTTGGAGCATTCTCATTC
  GAGAGGCACAGAATCAACCTATAGACAAAGCACGGAAGACTTATGAACGCCTTGTTGCCC
  AGTTCCCCAGTTCTGGCAGATTCTGGAAACTGTACATTGAAGCAGAGATTAAAGCTAAAA
  ATTATGACAAGGTTGAAAAGCTATTTCAGAGATGCCTTATGAAGGTTTTGCACATTGATT
  TATGGAAGTGTTATCTTTCATATGTCCGAGAAACCAAGGGTAAACTACCAAGTTACAAAG
  AAAAAATGGCTCAAGCATATGACTTTGCACTGGATAAAATTGGAATGGAAATTATGTCCT
  ATCAGATTTGGGTGGATTACATCAATTTCCTAAAAGGCGTGGAAGCTGTAGGATCTTATG
  CAGAAAATCAAAGAATAACAGCTGTCCGAAGAGTTTATCAACGAGGTTGTGTTAATCCGA
  TGATCAACATTGAACAGCTCTGGAGAGACTATAACAAGTATGAAGAGGGTATCAATATTC
  ATTTAGCTAAAAAATGATTGAAGATCGCAGTAGAGATTATATGAATGCTAGACGTGTAGC
  ANAGGGATATGAGACAGNNTATGAAAGGCTGGNACCCGTAATGCTCCTCCGTGCCTCCTC
  AGAATACTCCTCAGAGCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001326 unedited
  GTGGATCCTTTTTTTTTCTAAAGGACTTTCTAAAATTATGAAACTACAAAACGGATTTCA
  TACATTCTGCCATAAATAAAAACAAACAATAAGGCAGGACTCTACATAAGCAAACCAAGA
  ACAATTGTTTTCAGAAAATGTGATAAAATGCTTTACAATCATAAGCCATCCAGTTTTTAA
  AATAAAACTGTTGAAAAACTCACAAATCTTCAAGCGGCACATACAAGACACCCTTGAAGA
  GGGAACAAAATGTGGTTCTGCCACTTTGTACTGTTCTCACATTTTTGAAAACCTAATTTT
  GCTTATAGCATAGGTCTGTTCCGTATTCTAAAATTCACACAGGTTGGTTTGTTTTTTCTC
  AAGAACCATGTGATAGAGGCACCAATGACAATACAACTTTGTTTCCAAGAACCTTGTAAC
  AAAGCGTTGTCTCTTTTAAACATACCACTTGAGGCAAAAGGAATCCTGGACAGGAGTTTT
  CTGTAGAGGCGTTTAAAACCCTACCGAATCCGCTTCTGCTGCCGTGCTCTGTAAATGTCA
  TGAACAGGGGGGACAACGGCTCCCTTTTCTTCATCTTCATCTGAATCCTCGTTGGGCCTT
  TTGACGGCCTTGGTGAGTACTGCATTACTTTCCACGGGGCCGTTGCCTTCTACAGCTAGC
  TCTGGGGCCCCACCAGTAATGATCCTCACAGCTTCCTCAACAGTATTTGGTATCTTGCAT
  CTTCGGAAAATTTCCATCAGTTCATCCACTTGTACAAAAAGACCCTGNAAACAGATAGGA
  TGATGGAGATAGTTCATTAAACACAGCTGCNAGAGGACTGGGACACTCCACCAGTACATG
  GTGTAACCTGGAGTGCTAATGTCGGGCTGAATGGATCTCTGCTGATGTCTGTTTAAGGTA
  TCTAGTTTTCATCCACTCATCTTTANAACAGCCTATAAATGAGCTCACTGGGTCCGATAT
  AGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001326
• Insert Size: 3250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001326.1, NP_001317.1
• RefSeq Size: 2766 bp
• RefSeq ORF: 2154 bp
• Locus ID: 1479
• Cytogenetics: 11p13
• Domains: HAT
• Gene Summary: 'The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).