Cathepsin V (CTSV) (NM_001333) Human Untagged Clone

CAT#: SC119311

CTSL2 (untagged)-Human cathepsin L2 (CTSL2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTSV
• Synonyms: CATL2; CTSL2; CTSU
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001333, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCTTTCGCTCGTCCTGGCTGCCTTTTGCTTGGGAATAGCCTCCGCTGTTCCAAAATTTGACCAAA
  ATTTGGATACAAAGTGGTACCAGTGGAAGGCAACACACAGAAGATTATATGGCGCGAATGAAGAAGGATG
  GAGGAGAGCAGTGTGGGAAAAGAATATGAAAATGATTGAACTGCACAATGGGGAATACAGCCAAGGGAAA
  CATGGCTTCACAATGGCCATGAATGCTTTTGGTGACATGACCAATGAAGAATTCAGGCAGATGATGGGTT
  GCTTTCGAAACCAGAAATTCAGGAAGGGGAAAGTGTTCCGTGAGCCTCTGTTTCTTGATCTTCCCAAATC
  TGTGGATTGGAGAAAGAAAGGCTACGTGACGCCAGTGAAGAATCAGAAACAGTGTGGTTCTTGTTGGGCT
  TTTAGTGCGACTGGTGCTCTTGAAGGACAGATGTTCCGGAAAACTGGGAAACTTGTCTCACTGAGCGAGC
  AGAATCTGGTGGACTGTTCGCGTCCTCAAGGCAATCAGGGCTGCAATGGTGGCTTCATGGCTAGGGCCTT
  CCAGTATGTCAAGGAGAACGGAGGCCTGGACTCTGAGGAATCCTATCCATATGTAGCAGTGGATGAAATC
  TGTAAGTACAGACCTGAGAATTCTGTTGCTAATGACACTGGCTTCACAGTGGTCGCACCTGGAAAGGAGA
  AGGCCCTGATGAAAGCAGTCGCAACTGTGGGGCCCATCTCCGTTGCTATGGATGCAGGCCATTCGTCCTT
  CCAGTTCTACAAATCAGGCATTTATTTTGAACCAGACTGCAGCAGCAAAAACCTGGATCATGGTGTTCTG
  GTGGTTGGCTACGGCTTTGAAGGAGCAAATTCGAATAACAGCAAGTATTGGCTCGTCAAAAACAGCTGGG
  GTCCAGAATGGGGCTCGAATGGCTATGTAAAAATAGCCAAAGACAAGAACAACCACTGTGGAATCGCCAC
  AGCAGCCAGCTACCCCAATGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001333 unedited
  GCAGGTTTNGTAACCCCACCTCGAGTGTGGCGTTAAAAACATCCCCCACGAGGTAATCTC
  ATTTCTTGTTTGCTGATGGTGCCTGCGCCCCTGCGACGGCTGCTGGTTTTGAAACATGAA
  TCTTTCGCTCGTCCTGGCTGCCTTTTGCTTGGGAATAGCCTCCGCTGTTCCAAAATTTGA
  CCAAAATTTGGATACAAAGTGGTACCAGTGGATTGTTTACACAGAAGATTATATGGCGCG
  AATGAAGAATGATGGAGGAGAGCAGTGTGGGAAAAGAATATGAAAATGATTGAACTGCAC
  AATGGGGAATACAGCCAAGGGAAACATGGCTTCACAATGGCCATGAATGCTTTTGGTGAC
  ATGACCAATGAAGAATTCATGCAGATGATGGGTTGCTTTCGAAACCAGAAATTCAGGAAG
  GGGAAAGTGTTCCGTGAGCCTCTGTTTCTTGATCTTCCCAAATCTGTGGATTGGAGAAAG
  AAAGGCTACGTGACGCCAGTGAAGAATCAGAAACAGTGTGGTTCTTGTTGGGCTTTTAGT
  GCGACTGGTGCTCTTGAAGGACAGATGTTCCGGATAACTGGGAAACTTGTCTCACTGAGC
  GAGCAGAATCTGGTGGACTGTTCGCGTCCTCAAGGCAATCATGGCTGCAATGGTGGCTTC
  ATGGCTAGGGCCTTCCAGTATGTCAAGGAGAACGGAGGCCTGGACTCTGAGGAATCCTAT
  CCATATGTAGCAGTGGATGATATCTGTGAGTACAGACCTGAGAATTCTGTTGCTAATGAC
  ACTGGCTTCACAGTGGTCGCACCTGGAAATGAGTAGGCCCTGATGAAAGCAGTCGCACTG
  TGGGGCCCTTCTTCCGTGCTATGGATGCAGGCCATTCGTCCTTCCAGGTCTACAAATCAG
  GCATTTATTTTGGACCAGACTGCAGCACTAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001333 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA
  TATACGAATTAAAATTTATTTCAAACTGTTTTGTACATCTTTTTAAAAAATGAAAATTCA
  AAAGTCTTAAAATTAAGCAATGAGTCTTTGATATCATAAAGCTGGGTATAACAATAATTA
  AAGTAGGGGTAACATTTTACCCTTGTAAAAATGTCACAAAATTAAAATCTCAACTTGGAT
  CCTCAATGATTCAACTGGTTTATCTTACACAATAAGCGTTTGGTCAGTTTCAAAATAAAA
  TTTCCCCAAACATGCTGGCCTTAAGTCCTTCCTCCTCACCATCCATCAGCTCACACATTG
  GGGTAGCTGGCTGCTGTGGCGATTCCACAGGGGGTGGTCTTGGCTTTGGCTATTTTTACA
  TAGCCATTCGAGCCCCATTCTGGACCCCAGCTGTTTTTGACGAGCCAATACTTGCTGTTA
  TTCGAATTTGCTCCTTCAAAGCCGTAGCCAACCACCAGAACACCATGATCCAGGTTTTTG
  CTGCTGCAGTCTGGTTCAAAATAAATGCCTGATTTGTAGAACTGGAAGGACGAATGGCCT
  GCATCCATAGCAACGGAGATGGGCCCCACAGTTGCGACTGCTTTCATCAGGGCCTTCTCC
  TTTCCAGGTGCGACCACTGTGAAGCCAGTGTCATTACCAACAGATTCTAGGTCTGTACTA
  CAGATTCATCCCTGCTACTATGGTAGGATTCCTCAGATCCAAGCCTCCGTTTCCTGACTC
  TGAAAGCCTACCCTGAAGCCCCTTGCAACCTGATGCCTGGGACCGAACATCACCAATTTG
  TTCGTATGAAACAGTTCCAGTTTCGAAAATTGTCTAAGAGCCCGGCGACTAAGCCACAGA
  CCCTTGTTGATTTCCGGGGCCGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001333
• Insert Size: 1350 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001333.2, NP_001324.2
• RefSeq Size: 1496 bp
• RefSeq ORF: 1005 bp
• Locus ID: 1515
• Cytogenetics: 9q22.33
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: 'The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.