DAD1 (NM_001344) Human Untagged Clone

CAT#: SC119316

DAD1 (untagged)-Human defender against cell death 1 (DAD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAD1
• Synonyms: OST2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001344 edited
  ATGTCGGCGTCGGTAGTGTCTGTCATTTCGCGGTTCTTAGAAGAGTACTTGAGCTCCACT
  CCGCAGCGTCTGAAGTTGCTGGACGCGTACCTGCTGTATATACTGCTGACCGGGGCGCTG
  CAGTTCGGTTACTGTCTCCTCGTGGGGACCTTCCCCTTCAACTCTTTTCTCTCGGGCTTC
  ATCTCTTGTGTGGGGAGTTTCATCCTAGCGGTTTGCCTGAGAATACAGATCAACCCACAG
  AACAAAGCGGATTTCCAAGGCATCTCCCCAGAGCGAGCCTTTGCTGATTTTCTCTTTGCC
  AGCACCATCCTGCACCTTGTTGTCATGAACTTTGTTGGCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001344 unedited
  GCACGAGGTGTGGNTCGACGGGCTCCTCCAAGAGTTTGGGGCGCGGACCGGAGTACCTTG
  CGTGCAGTTATGTCGGCGTCGGTAGTGTCTGTCATTTCGCGGTTCTTAGAAGAGTACTTG
  AGCTCCACTCCGCAGCGTCTGAAGTTGCTGGACGCGTACCTGCTGTATATACTGCTGACC
  GGGGCGCTGCAGTTCGGTTACTGTCTCCTCGTGGGGACCTTCCCCTTCAACTCTTTTCTC
  TCGGGCTTCATCTCTTGTGTGGGGAGTTTCATCCTAGCGGTTTGCCTGAGAATACAGATC
  AACCCACAGAACAAAGCGGATTTCCAAGGCATCTCCCCAGAGCGAGCCTTTGCTGATTTT
  CTCTTTGCCAGCACCATCCTGCACCTTGTTGTCATGAACTTTGTTGGCTGAATCATTCTC
  ATTTACTTAATTGAGGAGTAGGAGACTAAAAGAATGTTCACTCTTTGAATTTCCTGGATA
  AGAGTTCTGGAGATGGCAGCTTATTGGACACATGGATTTTCTTCAGATTTGCACTTACTG
  CTAGCTCTGCTTTTTATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001344 unedited
  GGGGGGGGGCCNNNNCCCNCACCCCCCCAGGCCTTTTTGGCTGGAGTCCTTTTAATTGGT
  AGAGCAAAGGGTTACATTTCAATGGAACGGCAGCAGGCTGGCATTAATAAATGTTCGTTA
  CAAATTGTTCTGACACACAGTGAACTCTGGGCTTTTCTCCTGCATAAAAAGCAGAGCTAG
  CAGTAAGTTGCAAATCTGAAGAAAATCCATGTGTCCAATAAGCTGCCATCTCCAGAACTC
  TTATCCAGGAAATTCAAAGAGTGAACATTCTTTTATTCTCCTACTCCTCAATTAATTAAA
  TGAGAATGATTCAGCCAACAAAGTTCATGACAACAACGTGCACGATGGTGCTGGCAAACA
  GAAAATCATCTAAGGCTCGCTCTGGGGAGATGCCTCGGAAATCCGCTTTGTTCTGTGGGT
  TGATCTGTATTCTTATGCAAACCGCTATGATGAAACTTCCCCCCTATGATATGAATCCCC
  GACACAAAATAGCTGAACGGGAAGGTCCCCACGAGGAGACAGTAACCGAACTTGCCCGCC
  CCGGTTAGTAATATATACAGCTAGCACTCTTACTTCAACTTCTTACGCTGCGGCGCGCAC
  CTCAAATTCTTTTTCTACAACCGCCATATGTCACATACTACCCCCGTATTTCTTACCTGC
  CCTCAATGTTCTTCCGTCCCTCGTTTTATATTTTCCAACACCCCCCTTCCCCCACCTTGT
  GCCCAACTTTTCCATGCCTTTTTTTGTGTGTTTTTTAAATAATTCTTCCCCTTTCTCTAC
  CTACCTCCCGCTCCTCCTCCCCCTCCCACCGCCCACTCTATACTCCCCCTTTCTCTTCCC
  AGCGTCCCCCTTCACACACCCTCCCCACACACTCCCTACTCATCTTTAAGAGTCTATACT
  CCCCTACCTCTCACTTCACCTCTGTTACTATTCCTCTCATTATTTCTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001344
• Insert Size: 830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001344.1, NP_001335.1
• RefSeq Size: 699 bp
• RefSeq ORF: 342 bp
• Locus ID: 1603
• Cytogenetics: 14q11.2
• Domains: DAD
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]'