CLIC1 (NM_001288) Human Untagged Clone

CAT#: SC119324

CLIC1 (untagged)-Human chloride intracellular channel 1 (CLIC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLIC1
• Synonyms: CL1C1; G6; NCC27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119324 sequence for NM_001288 edited (data generated by NextGen Sequencing)
  ATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGGCTGGCAGTGATGGGGCCAAG
  ATTGGGAACTGCCCATTCTCCCAGAGACTGTTCATGGTACTGTGGCTCAAGGGAGTCACC
  TTCAATGTTACCACCGTTGACACCAAAAGGCGGACCGAGACAGTGCAGAAGCTGTGCCCA
  GGGGGGCAGCTCCCATTCCTGCTGTATGGCACTGAAGTGCACACAGACACCAACAAGATT
  GAGGAATTTCTGGAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAGCTCTGAAC
  CCTGAGTCCAACACAGCTGGGCTGGACATATTTGCCAAATTTTCTGCCTACATCAAGAAT
  TCAAACCCAGCACTCAATGACAATCTGGAGAAGGGACTCCTGAAAGCCCTGAAGGTTTTA
  GACAATTACTTAACATCCCCCCTCCCAGAAGAAGTGGATGAAACCAGTGCTGAAGATGAA
  GGTGTCTCTCAGAGGAAGTTTTTGGATGGCAACGAGCTCACCCTGGCTGACTGCAACCTG
  TTGCCAAAGTTACACATAGTACAGGTGGTGTGTAAGAAGTACCGGGGATTCACCATCCCC
  GAGGCCTTCCGGGGAGTGCATCGGTACTTGAGCAATGCCTACGCCCGGGAAGAATTCGCT
  TCCACCTGTCCAGATGATGAGGAGATCGAGCTCGCCTATGAGCAAGTGGCAAAGGCCCTC
  AAATAA
  
  Clone variation with respect to NM_001288.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001288 unedited
  GTGTATATTTGTATACGACTTACTATAGGGCGGCCGCGATTCGGCACGAGGGTCTGATTG
  AGCTTGTGTTGTGCTGAAGGGACAGCCCTGGGTCTAGGGGAGAGAGTCCCTGAGTGTGAG
  ACCCGCCTTCCCCGGTCCCAGCCCCTCCCAGTTCCCCCAGGGACGGCCACTTCCTGGTCC
  CCGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGGCTGGCAGTG
  ATGGGGCCAAGATTGGGAACTGCCCATTCTCCCAGAGACTGTTCATGGTACTGTGGCTCA
  AGGGAGTCACCTTCAATGTTACCACCGTTGACACCAAAAGGCGGACCGAGACAGTGCAGA
  AGCTGTGCCCAGGGGGGCAGCTCCCATTCCTGCTGTATGGCACTGAAGTGCACACAGACA
  CCAACAAGATTGAGGAATTTCTGGAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGG
  CAGCTCTGAACCCTGAGTCCAACACAGCTGGGCTGGACATATTTGCCAAATTTTCTGCCT
  ACATCAAGAATTCAAACCCAGCACTCAATGACAATCTGGAGAAGGGACTCCTGAAAGCCC
  TGAAGGTTTTAGACAATTACTTAACATCCCCCCTCCCAGAAGAAGTGGATGAAACCAGTG
  CTGAAGATGAAGGTGTCTCTCACAGGAAGTTTTTGGATGGCAACGAGCTCACCCTGGCTG
  ACTGCAACCTGTTGCCAAAGTTACACATAGTACAGGTCGGTGTGTAAGAAGTACCCGGGA
  TTCACCATTCCCCGAGGCCTTTCCGGGAGTGCATCGGTACTTGAGCCATGCCCACGCCCC
  GGGAAGAATTCGCTTTCACCTGTCCAGATGATGGAGAGATCGAGCTCGCCTATGAGCAAG
  TGGCAAAGCCCCTCAATAAACCCCTTCTGGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001288 unedited
  GAGTCGAGTTTTTTTTTTTTTTTTTTATTCTGTATTTTATTACTGAAATATGTTGTCCTA
  CTCATCCCACCCCACAATAAAAATCTGACCCAGGCCCCCCATTTCTTTCCCTCATCCCCT
  CTTCCACCACACCATCCCGGAACAAGTGCTCCAGGATTCCCTGCCCACTGGCCATTTTGG
  AGTGTGTCCATTGGGTAGCAATGTGGAAACCACCAGGGCCTTTGTGGAGAAAATGGAGGG
  GGTTGAGGGAGTCCCAGGAGGGGCTTATTTGAGGGCCTTTGCCACTTGCTCATAGGCGAG
  CTCGATCTCCTCATCATCTGGACAGGTGGAAGCGAATTCTTCCCGGGCGTAGGCATTGCT
  CAAGTACCGATGCACTCCCCGGAAGGCCTCGGGGATGGTGAATCCCCGGTACTTCTTACA
  CACCACCTGTACTATGTGTAACTTTGGCAACAGGTTGCAGTCAGCCAGGGTGAGCTCGTT
  GCCATCCAAAAACTTCCTCTGAGAGACACCTTCATCTTCAGCACTGGTTTCATCCACTCC
  TTCTGGGAGGGGGGATGNTAAGTAAATTGTCTAACACCTTAAGGCTTTCAGGAGTCCCTT
  CTCCAAATTGGCATTGAGTGCTGGGGTTCGAATTCTTGAATGTAGGCGAAAATCTGGCCA
  ATATGTCCACCCCAATTGTGTTGGACTAAGGGTTCAAGCTTCCACCTTGGGGTACCCTGG
  AAGGCACAAACCTTGCCTCCAAAATTCTCAATTTGCGGGGGGTTGGGGCACCTCATGGCT
  CCCAGAGGAATGGGAGGTTGCCCCCTGGGCCACACTCCGCCCTTGTTTTGGTTTCCTTTG
  GGGGGAACCGGCGGCACACCTAAGGCGGCCCCCTTCGCCCACAGACCATGAAAGAGTTTT
  GGTATATGGTACACGCTCATTGGGCCCCACCTGGCGCCTTATTAAAATTGACCCGCGGGC
  GCCGCCACCCTTGCCGATCCCGCACCATTATCGACCCCTCTTTGAACCCCGCGGGCCTGT
  CCCCGCCAGGCCGCATTCCTCCCGATCGCACCCTCTACACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001288
• Insert Size: 1210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288.4, NP_001279.2
• RefSeq Size: 1265 bp
• RefSeq ORF: 726 bp
• Locus ID: 1192
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Ion Channels: Other
• Gene Summary: 'Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) has an alternate exon in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.