CDC6 (NM_001254) Human Untagged Clone

CAT#: SC119336

CDC6 (untagged)-Human cell division cycle 6 homolog (S. cerevisiae) (CDC6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC6
• Synonyms: CDC18L; HsCDC6; HsCDC18; MGORS5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119336 sequence for NM_001254 edited (data generated by NextGen Sequencing)
  ATGCCTCAAACCCGATCCCAGGCACAGGCTACAATCAGTTTTCCAAAAAGGAAGCTGTCT
  CGGGCATTGAACAAAGCTAAAAACTCCAGTGATGCCAAACTAGAACCAACAAATGTCCAA
  ACCGTAACCTGTTCTCCTCGTGTAAAAGCCCTGCCTCTCAGCCCCAGGAAACGTCTGGGC
  GATGACAACCTATGCAACACTCCCCATTTACCTCCTTGTTCTCCACCAAAGCAAGGCAAG
  AAAGAGAATGGTCCCCCTCACTCACATACACTTAAGGGACGAAGATTGGTATTTGACAAT
  CAGCTGACAATTAAGTCTCCTAGCAAAAGAGAACTAGCCAAAGTTCACCAAAACAAAATA
  CTTTCTTCAGTTAGAAAAAGTCAAGAGATCACAACAAATTCTGAGCAGAGATGTCCACTG
  AAGAAAGAATCTGCATGTGTGAGACTATTCAAGCAAGAAGGCACTTGCTACCAGCAAGCA
  AAGCTGGTCCTGAACACAGCTGTCCCAGATCGGCTGCCTGCCAGGGAAAGGGAGATGGAT
  GTCATCAGGAATTTCTTGAGGGAACACATCTGTGGGAAAAAAGCTGGAAGCCTTTACCTT
  TCTGGTGCTCCTGGAACTGGAAAAACTGCCTGCTTAAGCCGGATTCTGCAAGACCTCAAG
  AAGGAACTGAAAGGCTTTAAAACTATCATGCTGAATTGCATGTCCTTGAGGACTGCCCAG
  GCTGTATTCCCAGCTATTGCTCAGGAGATTTGTCAGGAAGAGGTATCCAGGCCAGCTGGG
  AAGGACATGATGAGGAAATTGGAAAAACATATGACTGCAGAGAAGGGCCCCATGATTGTG
  TTGGTATTGGACGAGATGGATCAACTGGACAGCAAAGGCCAGGATGTATTGTACACGCTA
  TTTGAATGGCCATGGCTAAGCAATTCTCACTTGGTGCTGATTGGTATTGCTAATACCCTG
  GATCTCACAGATAGAATTCTACCTAGGCTTCAAGCTAGAGAAAAATGTAAGCCACAGCTG
  TTGAACTTCCCACCTTATACCAGAAATCAGATAGTCACTATTTTGCAAGATCGACTTAAT
  CAGGTATCTAGAGATCAGGTTCTGGACAATGCTGCAGTTCAATTCTGTGCCCGCAAAGTC
  TCTGCTGTTTCAGGAGATGTTCGCAAAGCACTGGATGTTTGCAGGAGAGCTATTGAAATT
  GTAGAGTCAGATGTCAAAAGCCAGACTATTCTCAAACCACTGTCTGAATGTAAATCACCT
  TCTGAGCCTCTGATTCCCAAGAGGGTTGGTCTTATTCACATATCCCAAGTCATCTCAGAA
  GTTGATGGTAACAGGATGACCTTGAGCCAAGAAGGAGCACAAGATTCCTTCCCTCTTCAG
  CAGAAGATCTTGGTTTGCTCTTTGATGCTCTTGATCAGGCAGTTGAAAATCAAAGAGGTC
  ACTCTGGGGAAGTTATATGAAGCCTACAGTAAAGTCTGTCGCAAACAGCAGGTGGCGGCT
  GTGGACCAGTCAGAGTGTTTGTCACTTTCAGGGCTCTTGGAAGCCAGGGGCATTTTAGGA
  TTAAAGAGAAACAAGGAAACCCGTTTGACAAAGGTGTTTTTCAAGATTGAAGAGAAAGAA
  ATAGAACATGCTCTGAAAGATAAAGCTTTAATTGGAAATATCTTAGCTACTGGATTGCCT
  TAA
  
  Clone variation with respect to NM_001254.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001254 unedited
  NCCGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTTGT
  GGTGGTGAGTCCGAGAGGCTGCGTGTGAGAGACGTGAGAAGGATCCTGCACTGAGGAGGT
  GGAAAGAAGAGGATTGCTCGAGGAGGCCTGGGGTCTGTGAGGCAGCGGAGCTGGGTGAAG
  GCTGCGGGTTCCGGCGAGGCCTGAGCTGTGCTGTCGTCATGCCTCAAACCCGATCCCAGG
  CACAGGCTACAATCAGTTTTCCAAAAAGGAAGCTGTCTCGGGCATTGAACAAAGCTAAAA
  ACTCCAGTGATGCCAAACTAGAACCAACAAATGTCCAAACCGTAACCTGTTCTCCTCGTG
  TAAAAGCCCTGCCTCTCAGCCCCAGGAAACGTCTGGGCGATGACAACCTATGCAACACTC
  CCCATTTACCTCCTTGTTCTCCACCAAAGCAAGGCAAGAAAGAGAATGGTCCCCCTCACT
  CACATACACTTAAGGGACGAAGATTGGTATTTGACAATCAGCTGACAATTAAGTCTCCTA
  GCAAAAGAGAACTAGCCAAAGTTCACCAAAACAAAATACTTTCTTCAGTTAGAAAAAGTC
  AAGAGATCACAACAAATTCTGAGCAGAGATGTCCACTGAAGAAAGAATCTGCATGTGTGA
  GACTATTCAAGCAAGAAGGCACTTGCTACCAGCAAGCAAAGCTGGTCCTGAACACAGCTG
  TCCCAGATCGGCTGCCTGCNCAGGAAAGGGAGATGGATGTCATCAGGAATTTCTTGAGGG
  AACACATCTGTGGGAAAAAGCTGGAAGCCTTTACCTTTCTGGTGCTCCTGGNACTGGAAA
  ACTGCCTGCTTAAGCCGNATTCTGNCAGACCTCAAGAAGGAACTGAAAGGCTTTAAACTA
  TCTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001254 unedited
  ACCGCGGGCCGCAATTTANAGTCGAGTTTTTTTTTTTTTTTTTTTGCAACTTGAAAATAA
  ATATATTCACAATCTGTAGAGTTATAGTCTCCTTATGACCCCAACGCCCTTGGGAGTGCC
  CAAGAAACTCAACGAGTCTAAAATGCCACAATGTTGTAGTATCTGCACCATGAACATAGT
  ATACTATACAAAGAAATAAATTCTTTAACCAGTGTCCTCCAAATCTCCATGTGGACTTGC
  TTGCATTTTTCTGGATCATTTTCATTCTTCTTACCCTAAACTTAAAGTTCTTAACAAAAT
  ACCTTAATGGCTGAGCATGGTGGCTCACGCCTATAATCCCAGCACTTAGGGAGGCCAAGG
  TGGGCAGATCACTTGAGGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTAAAACCCT
  GTCTCTACTAAAAATTAAAAAATTACCTGGGCGCGGTGGTGGGCACCTGTAATCCCAGCT
  ACTCGGGAGGCTGAGGCAAGAGAATCACTTCAACCTGGGAAGCGGGCGCTGTACTGAGCA
  AAGAACGCGCCATTGCACTCCAGCCTGTGCAACAGGGTGAGACGCGCCTCAAAAACAACA
  CCACCAAACACACACAACAAAAAAATCCCCACTCATGCTTGAGCCAACGAACAGGCAAAC
  ATCCACCCATTCCTCTACACAGATCTGTACCCTTGCCAGCACATTGGCTAAAGATACACA
  AGTGGCACAACCACCGACTGCAACAAAAAAGGCCTTCCTGGTAACCTTTAGGGCACAAAT
  AGTTACACCCATACATTTTACTCCGTGCTCATTTCAAAGAACCCTACACTAAACACCATT
  TGGCTCTAAGTCAAAACGGCTAATTTGCTTTCAGGCCCGAAGGGTCAACCCTTAAAGAAA
  ACTGTACTCTTATATGCCCCCGTACACTTTCCGTGGGGCGAACAAAAACATTACGGCATC
  CT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001254
• Insert Size: 2870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001254.3, NP_001245.1
• RefSeq Size: 3053 bp
• RefSeq ORF: 1683 bp
• Locus ID: 990
• Cytogenetics: 17q21.2
• Domains: AAA, AAA
• Protein Pathways: Cell cycle
• Gene Summary: 'The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]'