CDC20 (NM_001255) Human Untagged Clone

CAT#: SC119337

CDC20 (untagged)-Human cell division cycle 20 homolog (S. cerevisiae) (CDC20)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC20
• Synonyms: bA276H19.3; CDC20A; p55CDC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119337 sequence for NM_001255 edited (data generated by NextGen Sequencing)
  ATGGCACAGTTCGCGTTCGAGAGTGACCTGCACTCGCTGCTTCAGCTGGATGCACCCATC
  CCCAATGCACCCCCTGCGCGCTGGCAGCGCAAAGCCAAGGAAGCCGCAGGCCCGGCCCCC
  TCACCCATGCGGGCCGCCAACCGATCCCACAGCGCCGGCAGGACTCCGGGCCGAACTCCT
  GGCAAATCCAGTTCCAAGGTTCAGACCACTCCTAGCAAACCTGGCGGTGACCGCTATATC
  CCCCATCGCAGTGCTGCCCAGATGGAGGTGGCCAGCTTCCTCCTGAGCAAGGAGAACCAG
  CCTGAAAACAGCCAGACGCCCACCAAGAAGGAACATCAGAAAGCCTGGGCTTTGAACCTG
  AACGGTTTTGATGTAGAGGAAGCCAAGATCCTTCGGCTCAGTGGAAAACCACAAAATGCG
  CCAGAGGGTTATCAGAACAGACTGAAAGTACTCTACAGCCAAAAGGCCACTCCTGGCTCC
  AGCCGGAAGACCTGCCGTTACATTCCTTCCCTGCCAGACCGTATCCTGGATGCGCCTGAA
  ATCCGAAATGACTATTACCTGAACCTTGTGGATTGGAGTTCTGGGAATGTACTGGCCGTG
  GCACTGGACAACAGTGTGTACCTGTGGAGTGCAAGCTCTGGTGACATCCTGCAGCTTTTG
  CAAATGGAGCAGCCTGGGGAATATATATCCTCTGTGGCCTGGATCAAAGAGGGCAACTAC
  TTGGCTGTGGGCACCAGCAGTGCTGAGGTGCAGCTATGGGATGTGCAGCAGCAGAAACGG
  CTTCGAAATATGACCAGTCACTCTGCCCGAGTGGGCTCCCTAAGCTGGAACAGCTATATC
  CTGTCCAGTGGTTCACGTTCTGGCCACATCCACCACCATGATGTTCGGGTAGCAGAACAC
  CATGTGGCCACACTGAGTGGCCACAGCCAGGAAGTGTGTGGGCTGCGCTGGGCCCCAGAT
  GGACGACATTTGGCCAGTGGTGGTAATGATAACTTGGTCAATGTGTGGCCTAGTGCTCCT
  GGAGAGGGTGGCTGGGTTCCTCTGCAGACATTCACCCAGCATCAAGGGGCTGTCAAGGCC
  GTAGCATGGTGTCCCTGGCAGTCCAATGTCCTGGCAACAGGAGGGGGCACCAGTGATCGA
  CACATTCGCATCTGGAATGTGTGCTCTGGGGCCTGTCTGAGTGCCGTGGATGCCCATTCC
  CAGGTGTGCTCCATCCTCTGGTCTCCCCATTACAAGGAGCTCATCTCAGGCCATGGCTTT
  GCACAGAACCAGCTAGTTATTTGGAAGTACCCAACCATGGCCAAGGTGGCTGAACTCAAA
  GGTCACACATCCCGGGTCCTGAGTCTGACCATGAGCCCAGATGGGGCCACAGTGGCATCC
  GCAGCAGCAGATGAGACCCTGAGGCTATGGCGCTGTTTTGAGTTGGACCCTGCGCGGCGG
  CGGGAGCGGGAGAAGGCCAGTGCAGCCAAAAGCAGCCTCATCCACCAAGGCATCCGCTGA
  
  Clone variation with respect to NM_001255.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001255 unedited
  NGTCAACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGATTTTG
  TGGCCGGCCAGAGCGAAGGGTCCCTTTCTGTCCCCTGAGCACCGTCGCCTCCTTTCCTCC
  GGGCTCCGTAGGCACCAACTGCAAGGACCCCTCCCCCTGCGGGCGCTCCCATGGCACAGT
  TCGCGTTCGAGAGTGACCTGCACTCGCTGCTTCAGCTGGATGCACCCATCCCCAATGCAC
  CCCCTGCGCGCTGGCAGCGCAAAGCCAAGGAAGCCGCAGGCCCGGCCCCCTCACCCATGC
  GGGCCGCCAACCGATCCCACAGCGCCGGCAGGACTCCGGGCCGAACTCCTGGCAAATCCA
  GTTCCAAGGTTCAGACCACTCCTAGCAAACCTGGCGGTGACCGCTATATCCCCCATCGCA
  GTGCTGCCCAGATGGAGGTGGCCAGCTTCCTCCTGAGCAAGGAGAACCAGCCTGAAAACA
  GCCAGACGCCCACCAAGAAGGAACATCAGAAAGCCTGGGCTTTGAACCTGAACGGTTTTG
  ATGTAGAGGAAGCCAAGATCCTTCGGCTCAGTGGAAAACCACAAAATGCGCCAGAGGGTT
  ATCAGAACAGACTGAAAGTACTCTACAGCCAAAAGGCCACTCCTGGCTCCAGCCGGAAGA
  CCTGCCGTTACATTCCTTCCCTGCCAGACCGTATCCTGGATGCGCCTGAAATCCGAAATG
  ACTATTACCTGAACCTTGTGGNATTGGAGTTCNTGGGAATGTACTGGGCCGTGGCACTGG
  ACAACAGTGTGTACCTGTGGAGTGCAAGCTCTGGTGACATCCTGCAGCTTTTGCAATGGA
  GCAGCCTGGGGATATATATNCTCTGTGGCCTGGATCAAAGAGGGNCACTACTTGCTGTGG
  CACCANNCAGTGCTGAGTGCACTATGGGAAGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001255 unedited
  NTTTTGCTCTGGACCGGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTAAA
  AAAAAAACATGAAGGGAGACATGACTTTATTAGAAAAAAAAAAACAACTGAGGTGATGGG
  TTGGTCTTCAGCGGATGCCTTGGTGGATGAGGCTGCTTTTGGCTGCACTGGCCTTCTCCC
  GCTCCCGCCGCCGCGCAGGGTCCAACTCAAAACAGCGCCATAGCCTCAGGGTCTCATCTG
  CTGCTGCGGATGCCACTGTGGCCCCATCTGGGCTCATGGTCAGACTCAGGACCCGGGATG
  TGTGACCTTTGAGTTCAGCCACCTTGGCCATGGTTGGGTACTTCCAAATAACTAGCTGGT
  TCTGTGCAAAGCCATGGCCTGAGATGAGCTCCTTGTAATGGGGAGACCAGAGGATGGAGC
  ACACCTGGGAATGGGCATCCACGGCACTCAGACAGGCCCCAGAGCACACATTCCAGATGC
  GAATGTGTCGATCACTGGTGCCCCCTCCTGTTGCCAGGACATTGGACTGCCAGGGACACC
  ATGCTACGGCCTTGACAGCCCCTTGATGCTGGGTGAATGTCTGCAGAGGAACCCAGCCAC
  CCTCTCCAGGAGCACTAGGCCACACATTGACCAAGTTATCATTACCACCACTGGCCAAAT
  GTCGTCCATCTGGGGCCCAGCGCAGCCCACACACTTNCTGGNCTGTGNGCCACTCAGTGT
  GGCCACATGGNTGTTCTGCTACCCGACATCATGGTGGNTGGGATGTGGCCCAGACGTGAA
  CCACTGGACAGATATTAGCTGNTCCAGCTTAGGNAGCCCCACTCGNCAGAGTGACTGGTN
  CATATTCNNAAGCCCGTTCTGCTGCTGCACATCCCATAGCTGCACCTAGCACTTGCTGGT
  GCCACAGNNCNNAGTAGTGCCTTCTTGGATCCAGCACAGAGGTATATATATTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001255
• Insert Size: 960 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255.1, NP_001246.1
• RefSeq Size: 1686 bp
• RefSeq ORF: 1500 bp
• Locus ID: 991
• Cytogenetics: 1p34.2
• Domains: WD40
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Ubiquitin mediated proteolysis
• Gene Summary: 'CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]'