RCBTB2 (NM_001268) Human Untagged Clone

CAT#: SC119348

RCBTB2 (untagged)-Human regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2 (RCBTB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RCBTB2
• Synonyms: CHC1L; RLG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119348 sequence for NM_001268 edited (data generated by NextGen Sequencing)
  ATGGAAGAAGAACTTCCTCTTTTCTCTGGAGACAGTGGCAAGCCAGTACAGGCTACTCTG
  TCATCTTTGAAGATGTTAGATGTGGGAAAGTGGCCAATTTTTTCCCTTTGTTCTGAAGAA
  GAACTACAGTTAATTCGTCAGGCTTGTGTCTTTGGCAGTGCTGGCAATGAAGTTTTATAC
  ACTACAGTAAATGATGAGATTTTTGTGCTTGGCACAAACTGCTGTGGCTGTTTGGGGTTA
  GGTGACGTCCAGAGCACCATTGAACCTCGGAGACTGGATTCTTTAAATGGCAAAAAAATA
  GCCTGCCTCAGCTATGGGAGTGGTCCACATATTGTCCTTGCAACAACAGAAGGAGAAGTC
  TTTACCTGGGGTCATAATGCTTATAGCCAGCTGGGCAATGGGACAACTAATCATGGTTTA
  GTGCCCTGTCATATCTCTACTAATCTGTCAAACAAACAAGTCATTGAAGTTGCCTGTGGG
  TCTTACCATTCTTTGGTGCTAACATCTGATGGAGAGGTATTTGCCTGGGGTTATAATAAC
  TCTGGGCAGGTAGGATCTGGATCAACAGTTAATCAGCCAATCCCTCGAAGAGTCACTGGC
  TGCCTACAAAATAAAGTAGTTGTGACCATAGCATGTGGGCAGATGTGCTGCATGGCAGTA
  GTAGACACGGGGGAGGTCTATGTCTGGGGTTACAACGGAAACGGGCAGCTTGGACTCGGC
  AACAGTGGCAACCAGCCAACCCCTTGCAGAGTGGCAGCTTTGCAAGGCATCCGTGTCCAG
  AGGGTCGCCTGTGGCTACGCACACACATTAGTATTAACAGATGAAGGCCAAGTGTATGCT
  TGGGGCGCCAATTCTTATGGGCAGTTGGGCACTGGCAATAAAAGCAACCAGTCCTATCCT
  ACTCCTGTCACTGTGGAAAAGGACAGGATTATCGAGATTGCAGCCTGTCACTCCACACAC
  ACGTCTGCGGCCAAGACGCAGGGTGGGCACGTGTACATGTGGGGCCAGTGCCGGGGTCAG
  TCCGTGATCCTCCCGCACCTCACCCACTTCTCCTGCACTGACGACGTGTTTGCCTGCTTT
  GCCACGCCCGCCGTCACGTGGCGCCTCCTCTCCGTGGAACCTGATGACCACCTCACAGTG
  GCTGAGTCACTGAAGAGGGAATTTGACAACCCGGACACTGCAGACCTGAAGTTTCTAGTT
  GATGGAAAGTACATTTATGCACATAAAGTCCTTCTCAAGATTCGATGTGAGCATTTTCGT
  TCGTCATTGGAAGATAACGAGGATGATATTGTAGAAATGAGTGAATTTTCATATCCTGTT
  TACCGGGCCTTCCTGGAATACCTATACACAGACAGCATCAGCCTTTCTCCTGAGGAGGCA
  GTAGGACTGCTAGACTTGGCTACATTTTATAGAGAAAATCGTTTGAAAAAGCTCTGCCAA
  CAAACTATCAAGCAAGGCATCTGCGAGGAGAATGCCATCGCTCTGCTCTCGGCTGCGGTG
  AAGTATGATGCACAGGATTTAGAAGAATTCTGCTTCAGGTTTTGCATAAACCATCTGACT
  GTAGTAACACAAACATCAGGTTTTGCAGAAATGGACCATGATCTCCTGAAGAACTTTATC
  AGCAAAGCAAGCAGAGTTGGAGCCTTTAAAAATTGA
  
  Clone variation with respect to NM_001268.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001268 unedited
  GTTGTCGANATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGGAGTCA
  CATTAACTTTGCTCTANAAGACAACTTTACAAGGATCTAAAAGGAACAGGATTAAAGATG
  ACTGAATACTGGGTTCCAGAAATTTAAAACAATCAGCTTAGCAAATCATATATTCTTCTG
  TGGAGCTGAGAATTGATGTCCGCTCTTCCCCGTGATTTGGAACTTTCCAATCCCAGAGAA
  AAGTTGACAAAGGGACTGCCCAGGACTGAGTCCATATGGAAGAAGAACTTCCTCTTTTCT
  CTGGAGACAGTGGCAAGCCAGTACAGGCTACTCTGTCATCTTTGAAGATGTTAGATGTGG
  GAAAGTGGCCAATTTTTTCCCTTTGTTCTGAAGAAGAACTACAGTTAATTCGTCAGGCTT
  GTGTCTTTGGCAGTGCTGGCAATGAAGTTTTATACACTACAGTAAATGATGAGATTTTTG
  TGCTTGGCACAAACTGCTGTGGCTGTTTGGGGTTAGGTGACGTCCAGAGCACCATTGAAC
  CTCGGAGACTGGATTCTTTAAATGGCAAAAAAATAGCCTGCCTCAGCTATGGGAGTGGTC
  CACATATTGTCCTTGCAACAACAGAAGGAGAAGTCTTTACCTGGGGTCATAATGCTTATA
  GCCAGCTGGGCAATGGGACAACTAATCATGGTTTAGTGCCCTGTCATATCTCTACTAATC
  TGTCAAACAAACAAGTCATTGAAGTTGCCTGTGGGTCTTACCATTCTTTGGTGCTAACAT
  CTGATGGAGAAGTATTTGCCTGGGGTTATAATAACTNNCTGGCAGTANGATCTGGATCAA
  CAGTTAATCAGCCAATCCCTCGAAGATCACTGGCTGCCTACAAATAAAGTATTGTGACCA
  TAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001268 unedited
  CGCGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTCTGCTATCTGCAAATAAGC
  ACTTTATTACCAAATAGTTCAGCATCAGAACAAGAATTCTAATTCCAAAAGGGTTTCCTT
  TACATTACATGACATTGTGAGATCCACATTACAAGAATCTGAGACTATGCCCCATTCCAG
  CTGCTGTTTTGCAATCAGGCAATTCGCCTTGATCTCCCACCTCCAAATCCACCTCATTGT
  TGCTACCGCTGCCCATGAAATGTCCCGTTCAACAGCTTCCCACCTACCGCTTTCTGCCCA
  TCCTGCTGTCAGCCTCACACACCCATTCTATCACGCGCGCCCCGACTTCATTCTTCATCA
  ATTCGTCTCGGCCTTCCCTTTCATCTCGGGCCCCCCCGTCTCACCGTCGTTACGTACCTC
  CGTTTCCCCCTCCCCCCTCCTCCTCTCTACCTTCTACCTTCTCCCTCTTTCTCCTTCTTT
  CCCGCCTTTCCTCTTCTCTCATTTTACTTCCCCTCCCTTTTCTCCTCCCGTTCTTTCCCC
  CCGTTCCCCCCCCCCATTCTCCTTTCCCCCCCCTCCCGTCCCCACCTTTTCACTTTCCCC
  CGCCCCCCCTTTCTCCTCCCCCCCTGCCTTCCCCCGTTCTAATGCCTCCTCCTACCCTAC
  CATCCTCGCGTCGCGCGTGTATTTCTATCACCTATTATTCTTTCTCCTCTCTTCCACCGT
  TCCCCCGTTTCCTTCCTTTTCCCGCCTATCCGTCCTCCTTCCCCCTGTCCCGTCCATTCA
  CCCCCACCTTCCGTTTACCGTGCCGCTTACGTCCTCCCGCGCTTTCCTCGTCCATCCTAG
  TCGTTCCCACCTCTTTGCCACCCCCCCCTCCTCCTTTTACCCCACCCCCCCTATTCTTTA
  TCTCCACCACACCACCTCTCCGTAACTGGTTCTATGCCACCCATGCCCCCCGCGGCTTAA
  CTTGTCCCATTCGCTTCCCCTCCTTGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001268
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001268.2, NP_001259.1
• RefSeq Size: 3204 bp
• RefSeq ORF: 1656 bp
• Locus ID: 1102
• Cytogenetics: 13q14.2
• Domains: BTB, RCC1
• Gene Summary: 'This gene encodes a protein containing two C-terminal BTB/POZ domains that is related to regulator of chromosome condensation (RCC). The encoded protein may act as a guanine nucleotide exchange factor. This gene is observed to be lost or underexpressed in prostate cancers. There is a pseudogene of this gene on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]'
  Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 5, and 6 all encode the same isoform (2).