Caspase-6 (CASP6) (NM_001226) Human Untagged Clone

CAT#: SC119361

CASP6 (untagged)-Human caspase 6, apoptosis-related cysteine peptidase (CASP6), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASP6
• Synonyms: MCH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119361 sequence for NM_001226 edited (data generated by NextGen Sequencing)
  ATGAGCTCGGCCTCGGGGCTCCGCAGGGGGCACCCGGCAGGTGGGGAAGAAAACATGACA
  GAAACAGATGCCTTCTATAAAAGAGAAATGTTTGATCCGGCAGAAAAGTACAAAATGGAC
  CACAGGAGGAGAGGAATTGCTTTAATCTTCAATCATGAGAGGTTCTTTTGGCACTTAACA
  CTGCCAGAAAGGCGGGGCACCTGCGCAGATAGAGACAATCTTACCCGCAGGTTTTCAGAT
  CTAGGATTTGAAGTGAAATGCTTTAATGATCTTAAAGCAGAAGAACTACTGCTCAAAATT
  CATGAGGTGTCAACTGTTAGCCACGCAGATGCCGATTGCTTTGTGTGTGTCTTCCTGAGC
  CATGGCGAAGGCAATCACATTTATGCATATGATGCTAAAATCGAAATTCAGACATTAACT
  GGCTTGTTCAAAGGAGACAAGTGTCACAGCCTGGTTGGAAAACCCAAGATATTTATCATT
  CAGGCATGTCGGGGAAACCAGCACGATGTGCCAGTCATTCCTTTGGATGTAGTAGATAAT
  CAGACAGAGAAGTTGGACACCAACATAACTGAGGTGGATGCAGCCTCCGTTTACACGCTG
  CCTGCTGGAGCTGACTTCCTCATGTGTTACTCTGTTGCAGAAGGATATTATTCTCACCGG
  GAAACTGTGAACGGCTCATGGTACATTCAAGATTTGTGTGAGATGTTGGGAAAATATGGC
  TCCTCCTTAGAGTTCACAGAACTCCTCACACTGGTGAACAGGAAAGTTTCTCAGCGCCGA
  GTGGACTTTTGCAAAGACCCAAGTGCAATTGGAAAGAAGCAGGTTCCCTGTTTTGCCTCA
  ATGCTAACTAAAAAGCTGCATTTCTTTCCAAAATCTAATTAA
  
  Clone variation with respect to NM_001226.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001226 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGGCAAGGTGTCT
  GGCTGCGCGTTTGGCTGCAATGAGCTCGGCCTCGGGGCTCCGCAGGGGGCACCCGGCAGG
  TGGGGAAGAAAACATGACAGAAACAGATGCCTTCTATAAAAGAGAAATGTTTGATCCGGC
  AGAAAAGTACAAAATGGACCACAGGAGGAGAGGAATTGCTTTAATCTTCAATCATGAGAG
  GTTCTTTTGGCACTTAACACTGCCAGAAAGGCGGGGCACCTGCGCAGATAGAGACAATCT
  TACCCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGCTTTAATGATCTTAAAGCAGA
  AGAACTACTGCTCAAAATTCATGAGGTGTCAACTGTTAGCCACGCAGATGCCGATTGCTT
  TGTGTGTGTCTTCCTGAGCCATGGCGAAGGCAATCACATTTATGCATATGATGCTAAAAT
  CGAAATTCAGACATTAACTGGCTTGTTCAAAGGAGACAAGTGTCACAGCCTGGTTGGAAA
  ACCCAAGATATTTATCATTCAGGCATGTCGGGGAAACCAGCACGATGTGCCAGTCATTCC
  TTTGGATGTAGTAGATAATCAGACAGAGAAGTTGGACACCAACATAACTGAGGTGGATGC
  AGCCTCCGTTTACACGCTGCCTGCTGGAGCTGACTTCCTCATGTNGTACTCTGTTGCAGA
  AGGATATTATTCTCACCGGGAAACTGTGAACGGCTCATGGTACATTCAAGAATTGTGTGA
  GATGTTGGGAAAATATGGNNCTCTCCTTTAAAGTCACAGAACTCCTCACACTGGTGAACA
  NGNANAGTTCTCAGCGCCCGAGTGGACTTTNNGCAAGACCCNAGTGCANNTGGAAGNAAG
  NCAGNTCCCTGGTTTTGCCTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001226 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAATTTTGAATATTTTATTAA
  GGGATGATTTAATTCCTGGATTTCAAAAACCTTTAAAAACATCAAACAATAAACTTTTAT
  AAAAAAGTGACAAAATACAAGTTAAATCAGCTAGATTTTTGTGTAACCCTGCAGTTTATT
  AAAAAATAATACAAATGCTTATAAATTTTTATTTATCTAATTTCCTGAGGATTTTGTTTC
  TCCATATCAAACCCTTTCACCATGGCCAACATGAACTTTTTTTTTTTTTTTTTAAGGCAG
  TTCTCTGCTAGGCATTAAACTTTAAAACATTTGAATCATTGGACCATAATGCTTCACCCT
  AACGATATTTATATAAAAGGAAGAGAAAGACATTTTCTTTTTTTTGAGACGGAGTTTCAC
  TCGTTGCCCAGGCTGGAGTGCAATGGCGCAATCTCGGCTCACCGCAGCCTCCACCTCCTG
  GGTTCAAGTGATTCTCCTGCCTCAGCCCTCCAAGTAGCTGGGATTGCAGGCATGCGCCGC
  CATGCCTAGCTAAATTTTTTTTTGCATTTTTAGTAGAGACGGGGCTTCTCCATGTTGGTC
  AGGCTGGTCTCGAACTCCCGACCTCAGGTGATCCGCCCACCTTGGACTCCCAAAGTGCTG
  GGATTACAGGTGTGAGTAACCACGCCTGGCTGAGAAAGCCATTTTTAATACAGAGTGTAA
  AATTAGATAGCCCCTATTAATTAATTAGATTTCTGGAAAGAAATGCAGCTCTTTAGTTAG
  CATTGAGGCAAACAGGGAACCTGCTTCTTTCCATTGCACTTGGGTCTTTGCAAAGTCCCT
  TGGGGCTGAAAACTTCCTGTCACCATGTGAGGGTTTGTGACTCTAAGGAGAGCATATTTT
  CCACATTTACAAAATTTGATGTCCATGACCGTACAAGTTCCGGGAGATAATTTTCTGCAC
  AATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001226
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001226.3, NP_001217.2
• RefSeq Size: 1661 bp
• RefSeq ORF: 882 bp
• Locus ID: 839
• Cytogenetics: 4q25
• Domains: CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, Protease, Stem cell - Pluripotency
• Protein Pathways: Apoptosis
• Gene Summary: 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.