CASQ2 (NM_001232) Human Untagged Clone

CAT#: SC119365

CASQ2 (untagged)-Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASQ2
• Synonyms: PDIB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119365 sequence for NM_001232 edited (data generated by NextGen Sequencing)
  ATGAAGAGAACTCACTTGTTTATTGTGGGGATTTATTTTCTGTCCTCTTGCAGGGCAGAA
  GAGGGGCTTAATTTCCCCACATATGATGGGAAGGACCGAGTGGTAAGTCTTTCCGAGAAG
  AACTTCAAGCAGGTTTTAAAGAAATATGACTTGCTTTGCCTCTACTACCATGAGCCGGTG
  TCTTCAGATAAGGTCACGCAAAAACAGTTCCAACTGAAAGAAATCGTGCTTGAGCTTGTG
  GCCCAGGTCCTTGAACATAAAGCTATAGGCTTTGTGATGGTGGATGCCAAGAAAGAAGCC
  AAGCTTGCCAAGAAACTGGGTTTTGATGAAGAAGGAAGCCTGTATATTCTTAAGGGTGAT
  CGCACAATAGAGTTTGATGGCGAGTTTGCAGCTGATGTCTTGGTGGAGTTCCTCTTGGAT
  CTAATTGAAGACCCAGTGGAGATCATCAGCAGCAAACTGGAAGTCCAAGCCTTCGAACGC
  ATTGAAGACTACATCAAACTCATTGGCTTTTTCAAGAGTGAGGACTCAGAATACTACAAG
  GCTTTTGAAGAAGCAGCTGAACACTTCCAGCCTTACATCAAATTCTTTGCCACCTTTGAC
  AAAGGGGTTGCAAAGAAATTATCTTTGAAGATGAATGAGGTTGACTTCTATGAGCCATTT
  ATGGATGAGCCCATTGCCATCCCCAACAAACCTTACACAGAAGAGGAGCTGGTGGAGTTT
  GTGAAGGAACACCAAAGACCCACTCTACGTCGCCTGCGCCCAGAAGAAATGTTTGAAACA
  TGGGAAGATGATTTGAATGGGATCCACATTGTGGCCTTTGCAGAGAAGAGTGATCCAGAT
  GGCTACGAATTCCTGGAGATCCTGAAACAGGTTGCCCGGGACAATACTGACAACCCCGAT
  CTGAGCATCCTGTGGATCGACCCGGACGACTTTCCTCTGCTCGTTGCCTACTGGGAGAAG
  ACTTTCAAGATTGACCTATTCAGGCCACAGATTGGGGTGGTGAATGTCACAGATGCTGAC
  AGTGTCTGGATGGAGATTCCAGATGATGACGATCTTCCAACTGCTGAGGAGCTGGAGGAC
  TGGATTGAGGATGTGCTTTCTGGAAAGATAAACACTGAAGATGATGATGAAGATGATGAT
  GATGATGATAATTCTGATGAAGAGGATAATGATGACAGTGATGACGATGATGATGAATAG
  
  Clone variation with respect to NM_001232.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001232 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAAGAGTCACTTTTCAGC
  CCCAGGAAGGGCAAAGAAGAGAGAAAATCAGCCTGTCTGCTCTCTCCTTGGCTCAACAAG
  GCCTCTAACAGTCTTCTGTCCTCTATTCTGCACACGGCATATTTGGGAACGAGAAACAAA
  AGTTTTCCCAAATGAAGAGAACTCACTTGTTTATTGTGGGGATTTATTTTCTGTCCTCTT
  GCAGGGCAGAAGAGGGGCTTAATTTCCCCACATATGATGGGAAGGACCGAGTGGTAAGTC
  TTTCCGAGAAGAACTTCAAGCAGGTTTTAAAGAAATATGACTTGCTTTGCCTCTACTACC
  ATGAGCCGGTGTCTTCAGATAAGGTCACGCAAAAACAGTTCCAACTGAAAGAAATCGTGC
  TTGAGCTTGTGGCCCAGGTCCTTGAACATAAAGCTATAGGCTTTGTGATGGTGGATGCCA
  AGAAAGAAGCCAAGCTTGCCAAGAAACTGGGTTTTGATGAAGAAGGAAGCCTGTATATTC
  TTAAGGGTGATCGCACAATAGAGTTTGATGGCGAGTTTGCAGCTGATGTCTTGGNTGGAG
  TTCCTTCTTGGATCTAATTGAAGACCCAGTGGGAGATCATCAGCAGCAAACTTGGAAGTC
  CAAGCCTTCGAACGCATTGAAGACTACATCAAACTCATTGGGCTTTTTCAAGAAGGAGGA
  CTCAGAATACTTACAAGCTTTTGAAGAACAGCTGACACTTCCAGCCTTACTCAATTCTTT
  GCCCCTTTGACCAAAGGGGTGCAAGAAATTATCTTGAGATGAAGAAGGTTGACTTCTATG
  ACCTTTTGGATGAACCCTTGGCATCCAACAACTAACAAGAGAGATGGGGGGTGTTTTGGA
  GGAACCAAGACCACTTTATCGCTGGCAAAAAAGGTGAACTGGAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001232 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTTCAAAATATGAGTTT
  AATGACAGAATTAGTTAGCTAGTATTCCACAAAAAGTATTGCTCTATTTTCAAAAAATTT
  GCACAGTGTCTTACACATGTGCTAAAAGATTGAGAAAATAAATTAGAAAATTATACTGCA
  CACTTAACACTAAATCTACCAAGCACAATGTAACTTTTAGACAGCTCAGAAGGCACTTTG
  GGATTTTTTTTTTTTTCAGTGCCTCAGGGCATCAGTATGAACTCCAATTATTGTTGCCCT
  GGCCAATTGTGGGAGTACTGATAACTGGAGAGTTAATTGACTGCTGGATAAAGCAATCTT
  TAATCTAAATGGGAAGGCTCACTAGCAGCTACAGAGAAGGGGTATTCAGATCCCAGCTTA
  GGCTAGGAAGCCAGCTGACCCAATCAGAGACATGAACCCATCAGAAAAATGTAAAAGTTT
  TCATCTTTCTAGTTTTCCCCCACCCTCCACTCCTGCTGATGAATATGTGAGACTTGCCAA
  CTAGAGGGAAGTTTAGAGAGCAAATCAACCTCCCATCCCAGTTTCCTAATATACTAGAAA
  TGATGGTGACACCTGTACACATTTCAAGCCCTCTCCATAGCCATCCAAAGGCTTGAATAT
  CCAAGTTCATAGTGATATGAATGACCATCACTTGAAATAAGTTTAACAAAGCCATGAGAA
  TATGATTTATAAGTTTGCTTCCAAGGCTGAGCTTNCTCTAAGAAGGATCATCTGGNCTGG
  AGGAGGGATCCNNACTGATNGTCGAGGTATGGAGGGAGCTAAATCATAATCATGGGGCTN
  CCTGGGCTCTGATAAAAAGTCACTCTATGCCTGTGAGTAAAAGCTGCAATTNTGTACTGG
  CATATCAAGACAGNAAACTGGGTAGGGGATGTTCCCCTANATGGCTGTCAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001232
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001232.1, NP_001223.1
• RefSeq Size: 2528 bp
• RefSeq ORF: 1200 bp
• Locus ID: 845
• Cytogenetics: 1p13.1
• Domains: Calsequestrin
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq, Jul 2008]'