ATP6AP1 (NM_001183) Human Untagged Clone

CAT#: SC119382

ATP6AP1 (untagged)-Human ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1)


  "NM_001183" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ATP6AP1
Synonyms 16A; Ac45; ATP6IP1; ATP6S1; CF2; VATPS1; XAP-3; XAP3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001183 edited
GAATTCGGCACCAGGAGGCTATGATGGCGGCCATGGCGACGGCTCGAGTGCGGATGGGGC
CGCGATGCGCCCAGGCGCTCTGGCGCATGCCGTGGCTGCCGGTGTTTTTGTCGTTGGCGG
CGGCGGCGGCGGCGGCAGCGGCGGAGCAGCAGGTCCCGCTGGTGCTGTGGTCGAGTGACC
GGGACTTGTGGGCTCCTGCGGCCGACACTCATGAAGGCCACATCACCAGCGACTTGCAGC
TCTCTACCTACTTAGATCCCGCCCTGGAGCTGGGTCCCAGGAATGTGCTGCTGTTCCTGC
AGGACAAGCTGAGCATTGAGGATTTCACAGCATATGGCGGTGTGTTTGGAAACAAGCAGG
ACAGCGCCTTTTCTAACCTAGAGAATGCCCTGGACCTGGCCCCCTCCTCACTGGTGCTTC
CTGCCGTCGACTGGTATGCAGTCAGCACTCTGACCACTTACCTGCAGGAGAAGCTCGGGG
CCAGCCCCTTGCATGTGGACCTGGCCACCCTGCGGGAGCTGAAGCTCAATGCCAGCCTCC
CTGCTCTGCTGCTCATTCGCCTGCCCTACACAGCCAGCTCTGGTCTGATGGCACCCAGGG
AAGTCCTCACAGGCAACGATGAGGTCATCGGGCAGGTCCTGAGCACACTCAAGTCCGAAG
ATGTCCCATACACAGCGGCCCTCACAGCGGTCCGCCCTTCCAGGGTGGCCCGTGATGTAG
CCGTGGTGGCCGGAGGGCTAGGTCGCCAGCTGCTACAAAAACAGCCAGTATCACCTGTGA
TCCATCCTCCTGTGAGTTACAATGACACCGCTCCCCGGATCCTGTTCTGGGCCCAAAACT
TCTCTGTGGCGTACAAGGACCAGTGGGAGGACCTGACTCCCCTCACCTTTGGGGTGCAGG
AACTCAACCTGACTGGCTCCTTCTGGAATGACTCCTTTGCCAGGCTCTCACTGACCTATG
AACGACTCTTTGGTACCACAGTGACATTCAAGTTCATTCTGGCCAACCGCCTCTACCCAG
TGTCTGCCCGGCACTGGTTTACCATGGAGCGCCTCGAAGTCCACAGCAATGGCTCCGTCG
CCTACTTCAATGCTTCCCAGGTCACAGGGCCCAGCATCTACTCCTTCCACTGCGAGTATG
TCAGCAGCCTGAGCAAGAAGGGTAGTCTCCTCGTGGCCCGCACGCAGCCCTCTCCCTGGC
AGATGATGCTTCAGGACTTCCAGATCCAGGCTTTCAACGTAATGGGGGAGCAGTTCTCCT
ACGCCAGCGACTGTGCCAGCTTCTTCTCCCCCGGCATCTGGATGGGGCTGCTCACCTCCC
TGTTCATGCTCTTCATCTTCACCTATGGCCTGCACATGATCCTCAGCCTCAAGACCATGG
ATCGCTTTGATGACCACAAGGGCCCCACTATTTCTTTGACCCAGATTGTGTGACCCTGTG
CCAGTGGGGGGGTTGAGGGTGGGACGGTGTCCGTGTTGTTGCTTTCCCACCCTGCAGCGC
ACTGGACTGAAGAGCTTCCCTCTTCCTACTGCAGCATGAACTGCAAGCTCCCCTCAGCCC
ATCTTGCTCCCTCTTCAGCCCGCTGAGGAGCTTTCTTGGGCTGCCCCCATCTCTCCCAAC
AAGGTGTACATATTCTGCGTAGATGCTAGACCAACCAGCTTCCCAGGGTTCGTCGCTGTG
AGGCGTAAGGGACGTGAATTCTAGGGTCTCCTTTCTCCTTATTTATTCTTGTGGCTACAT
CATCCCTGGCTGTGGATAGTGCTTTTGTGTAGCAAATGCTCCCTCCTTAAGGTTATAGGG
CTCCCTGAGTTTGGGAGTGTGGAAGTACTACTTAACTGTCTGTCCTGCTTGGCTGTCGTT
ATCGTTTTCTGGTGATGTTGTGCTAACAATAAGCAGTACACGGGTTTATTTCTGTGGCCT
GAGAAGGAAGGGACCTCCACGACAGGTGGGCTGGGTGCGATCGCCGGCTGTTTGGCATGT
TCCCACCGGGAGTGCCGGGCAGGAGCATGGGGTGCTTGGTTGTTTCCTTCCTAATAAAAT
AAACGCGGGTCGCCATGAAAAAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_001183 unedited
NTTGTTAGATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGAGGCTAT
GATGGCGGCCATGGCGACGGCTCGAGTGCGGATGGGGCCGCGATGCGCCCAGGCGCTCTG
GCGCATGCCGTGGCTGCCGGTGTTTTTGTCGTTGGCGGCGGCGGCGGCGGCGGCAGCGGC
GGAGCAGCAGGTCCCGCTGGTGCTGTGGTCGAGTGACCGGGACTTGTGGGCTCCTGCGGC
CGACACTCATGAAGGCCACATCACCAGCGACTTGCAGCTCTCTACCTACTTAGATCCCGC
CCTGGAGCTGGGTCCCAGGAATGTGCTGCTGTTCCTGCAGGACAAGCTGAGCATTGAGGA
TTTCACAGCATATGGCGGTGTGTTTGGAAACAAGCAGGACAGCGCCTTTTCTAACCTAGA
GAATGCCCTGGACCTGGCCCCCTCCTCACTGGTGCTTCCTGCCGTCGACTGGTATGCAGT
CAGCACTCTGACCACTTACCTGCAGGAGAAGCTCGGGGCCAGCCCCTTGCATGTGGACCT
GGCCACCCTGCGGGAGCTGAAGCTCAATGCCAGCCTCCCTGCTCTGCTGCTCATTCGCCT
GCCCTACACAGCCAGCTCTGGTCTGATGGCACCCAGGGAAGTCCTCACAGGCAACGATGA
GGTCATCGGGCAGGTCCTGAGCACACTCAAGTCCGAAGATGTCCCATACACAGCGGCCCT
CACAGCGGTCCGCCCTTCCAGGTGGCCCGTGATGTANCCGTGGTGGCCGGAGGGCTAGGT
CGCCAGCTGCTACAAAACAGCCAGATCACCTGTGATCATNCTCCTGTGANTACATGACAC
GCTCCCNNGGATCTGNTCTGGCCCAAACTTCTCTGTGCGTACAGGACANTGGNNAGACTG
ACTCCTACTTTTGGGGGCAGAA
Restriction Sites NotI-NotI     
ACCN NM_001183
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001183.3, NP_001174.2
RefSeq Size 2055 bp
RefSeq ORF 1413 bp
Locus ID 537
Cytogenetics Xq28
Protein Families Transmembrane
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary 'This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development. [provided by RefSeq, Aug 2013]'

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