cIAP2 (BIRC3) (NM_001165) Human Untagged Clone

CAT#: SC119402

BIRC3 (untagged)-Human baculoviral IAP repeat containing 3 (BIRC3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BIRC3
• Synonyms: AIP1; API2; c-IAP2; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; MIHC; RNF49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119402 sequence for NM_001165 edited (data generated by NextGen Sequencing)
  ATGAACATAGTAGAAAACAGCATATTCTTATCAAATTTGATGAAAAGCGCCAACACGTTT
  GAACTGAAATACGACTTGTCATGTGAACTGTACCGAATGTCTACGTATTCCACTTTTCCT
  GCTGGGGTTCCTGTCTCAGAAAGGAGTCTTGCTCGTGCTGGTTTCTATTACACTGGTGTG
  AATGACAAGGTCAAATGCTTCTGTTGTGGCCTGATGCTGGATAACTGGAAAAGAGGAGAC
  AGTCCTACTGAAAAGCATAAAAAGTTGTATCCTAGCTGCAGATTCGTTCAGAGTCTAAAT
  TCCGTTAACAACTTGGAAGCTACCTCTCAGCCTACTTTTCCTTCTTCAGTAACAAATTCC
  ACACACTCATTACTTCCGGGTACAGAAAACAGTGGATATTTCCGTGGCTCTTATTCAAAC
  TCTCCATCAAATCCTGTAAACTCCAGAGCAAATCAAGATTTTTCTGCCTTGATGAGAAGT
  TCCTACCACTGTGCAATGAATAACGAAAATGCCAGATTACTTACTTTTCAGACATGGCCA
  TTGACTTTTCTGTCGCCAACAGATCTGGCAAAAGCAGGCTTTTACTACATAGGACCTGGA
  GACAGAGTGGCTTGCTTTGCCTGTGGTGGAAAATTGAGCAATTGGGAACCGAAGGATAAT
  GCTATGTCAGAACACCTGAGACATTTTCCCAAATGCCCATTTATAGAAAATCAGCTTCAA
  GACACTTCAAGATACACAGTTTCTAATCTGAGCATGCAGACACATGCAGCCCGCTTTAAA
  ACATTCTTTAACTGGCCCTCTAGTGTTCTAGTTAATCCTGAGCAGCTTGCAAGTGCGGGT
  TTTTATTATGTGGGTAACAGTGATGATGTCAAATGCTTTTGCTGTGATGGTGGACTCAGG
  TGTTGGGAATCTGGAGATGATCCATGGGTTCAACATGCCAAGTGGTTTCCAAGGTGTGAG
  TACTTGATAAGAATTAAAGGACAGGAGTTCATCCGTCAAGTTCAAGCCAGTTACCCTCAT
  CTACTTGAACAGCTGCTATCCACATCAGACAGCCCAGGAGATGAAAATGCAGAGTCATCA
  ATTATCCATTTTGAACCTGGAGAAGACCATTCAGAAGATGCAATCATGATGAATACTCCT
  GTGATTAATGCTGCCGTGGAAATGGGCTTTAGTAGAAGCCTGGTAAAACAGACAGTTCAG
  AGAAAAATCCTAGCAACTGGAGAGAATTATAGACTAGTCAATGATCTTGTGTTAGACTTA
  CTCAATGCAGAAGATGAAATAAGGGAAGAGGAGAGAGAAAGAGCAACTGAGGAAAAAGAA
  TCAAATGATTTATTATTAATCCGGAAGAATAGAATGGCACTTTTTCAACATTTGACTTGT
  GTAATTCCAATCCTGGATAGTCTACTAACTGCCGGAATTATTAATGAACAAGAACATGAT
  GTTATTAAACAGAAGACACAGACGTCTTTACAAGCAAGAGAACTGATTGATACGATTTTA
  GTAAAAGGAAATATTGCAGCCACTGTATTCAGAAACTCTCTGCAAGAAGCTGAAGCTGTG
  TTATATGAGCATTTATTTGTGCAACAGGACATAAAATATATTCCCACAGAAGATGTTTCA
  GATCTACCAGTGGAAGAACAATTGCGGAGACTACAAGAAGAAAGAACATGTAAAGTGTGT
  ATGGACAAAGAAGTGTCCATAGTGTTTATTCCTTGTGGTCATCTAGTAGTATGCAAAGAT
  TGTGCTCCTTCTTTAAGAAAGTGTCCTATTTGTAGGAGTACAATCAAGGGTACAGTTCGT
  ACATTTCTTTCATGA
  
  Clone variation with respect to NM_001165.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001165 unedited
  GCGTTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGACGAATTCGCACGAGGAAAG
  ATATACAAAGGTGCAATTGTGTATTTCTTCCTTAAAAGTATCAGTATAGGATTTAGAATC
  TCCATGTTGAAACTCTAAATGCATAGAAATAAAAATAATAAAAAATTTTTCATTTTGGCT
  TTTCAGCCTAGTATTAAAACTGATAAAAGCAAAGCCATGCACAAAACTACCTCCCTAGAG
  AAAGGCTAGTCCCTTTTCTTCCCCATTCATTTCATTATGAACATAGTAGAAAACAGCATA
  TTCTTATCAAATTTGATGAAAAGCGCCAACACGTTTGAACTGAAATACGACTTGTCATGT
  GAACTGTACCGAATGTCTACGTATTCCACTTTTCCTGCTGGGGTTCCTGTCTCATAAAGG
  AGTCTTGCTCGTGCTGGTTTCTATTACACTGGTGTGAATGACAAGGTCAAATGCTTCTGT
  TGTGGCCTGATGCTGGATAACTGGAAAAGAGGAGACAGTCCTACTGAAAAGCATAAAAAG
  TTGTATCCTAGCTGCAGATTCGTTCAGAGTCTAAATTCCGTTAACAACTTGGAAGCTACC
  TCTCAGCCTACTTTTCCTTCTTCAGTAACAAATTCCACACACTCATTACTTCCGGGTACA
  GAAAACAGTGGATANTTTCCGTNGGCTCTTATTCAAACTCTCCATCAAATCCTGTAAACT
  CCAGAGCANATCAAGATTTTTCTGCCTTGATGAGAAGTTCCTACCACTGGTGCATGAATA
  ACGAANATGCCAGATTACTTACTTTTCAGAATGGCCATTGACTTTTCTGTCGCCAACAGA
  TCTGGGCAAAGCANGCTTTTACTACATANGACCTGGANACAGAGTGGCTTGCTTTGCCTG
  TGNTGGAAAAATGAGCATTNGGAACCGAAGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001165 unedited
  GGGGGCGCGNNNNNGNNNTTTTCNNNNNNNNNNTTTTGTTTAGNACCNCGGNCCCGCATN
  TAANNGATCGGTTTTTTTTTTTTTTTTTTAAAATAATTTTTGTTGCTTTATTAAATTTTT
  TCTCACAGAATTCTTTATAAAAACACCATGGTCCCTAAAATGTCATTCAACATATATGCA
  CACCTTCGATGTATAGGACACTGATCAAAAAAGACAGAGAAATGTGTCCCTGGTGTTTTG
  TTTTTGTTCTGTTTGTTTTTAAAGGCAGGGTCTCAGTATGCTGCCCAGGATGGATTCAAA
  CTCCTGGGCTCAAGTAATTCTCTTGCCTCAGCCTGGGACTACAGGCACATACCACCACAC
  CTGGCTTCATGTTCCCGGTATTAGTACAATGCCAAAATATTTAAAATTCTTAAAGGTTAA
  CTCAAATATCTTAAGTTTTACTTCACTTACAATTTCAATAATGCTGAAATTTTGATTGAA
  TATTGTGTTTGTAGTGCTACCTCTTTTTCGTTCATAAGAACAAAAGCCTATCATTCTCTT
  AGTTTCTAAAAAATATATGTTCATATGGTTTAGATACATATATAAATATGTTACACANAA
  CAATGTTTTTTGAGTTGTAAATAAATAAAAATTTTAAGGAAACCAAATAGGATAAAAGTT
  AAAGNTATAATACATTTAATAAATTAATTCTAAAGNTTAGATGATGNTTTGGTTCTTCCT
  CATGAAAGAAATGTACGAACTGTACCCCTTGGATGGACTCCTACAAATAGACAACTTTCT
  AAANGAANGAGCCCAATCTTTGCCTACTACTAGATGACCCCAGGAATAAAACCCTTTGGA
  CACTCCTTTGNCCATACCACTTTACAATGTCTTTCTCCTGAAGCCTCCGCAATTGTTCTT
  CCACTGTAAGAACTGAAACATCTTCTGGGGGAATTAATTTATGCCCTGTTGCCCAAAAAA
  TGCCAATTACCAAGTTTAACTTTCTTGAAAAAGGTTTGAAACAAGGGCTGAAATTTCCTT
  TACTAAAACGAAAATCATTTCTTGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001165
• Insert Size: 2620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165.3, NP_001156.1
• RefSeq Size: 5243 bp
• RefSeq ORF: 1815 bp
• Locus ID: 330
• Cytogenetics: 11q22.2
• Domains: CARD, BIR, RING
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Focal adhesion, NOD-like receptor signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.