Annexin VI (ANXA6) (NM_001155) Human Untagged Clone

CAT#: SC119425

ANXA6 (untagged)-Human annexin A6 (ANXA6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANXA6
• Synonyms: ANX6; CBP68; CPB-II; p68; p70
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119425 sequence for NM_001155 edited (data generated by NextGen Sequencing)
  ATGGCCAAACCAGCACAGGGTGCCAAGTACCGGGGCTCCATCCATGACTTCCCAGGCTTT
  GACCCCAACCAGGATGCCGAGGCTCTGTACACTGCCATGAAGGGCTTTGGCAGTGACAAG
  GAGGCCATACTGGACATAATCACCTCACGGAGCAACAGGCAGAGGCAGGAGGTCTGCCAG
  AGCTACAAGTCCCTCTACGGCAAGGACCTCATTGCTGATTTAAAGTATGAATTGACGGGC
  AAGTTTGAACGGTTGATTGTGGGCCTGATGAGGCCACCTGCCTATTGTGATGCCAAAGAA
  ATTAAAGATGCCATCTCGGGCATTGGCACTGATGAGAAGTGCCTCATTGAGATCTTGGCT
  TCCCGGACCAATGAGCAGATGCACCAGCTGGTGGCAGCATACAAAGATGCCTACGAGCGG
  GACCTGGAGGCTGACATCATCGGCGACACCTCTGGCCACTTCCAGAAGATGCTTGTGGTC
  CTGCTCCAGGGAACCAGGGAGGAGGATGACGTAGTGAGCGAGGACCTGGTACAACAGGAT
  GTCCAGGACCTATACGAGGCAGGGGAACTGAAATGGGGAACAGATGAAGCCCAGTTCATT
  TACATCTTGGGAAATCGCAGCAAGCAGCATCTTCGGTTGGTGTTCGATGAGTATCTGAAG
  ACCACAGGGAAGCCGATTGAAGCCAGCATCCGAGGGGAGCTGTCTGGGGACTTTGAGAAG
  CTAATGCTGGCCGTAGTGAAGTGTATCCGGAGCACCCCGGAATATTTTGCTGAAAGGCTC
  TTCAAGGCTATGAAGGGCCTGGGGACTCGGGACAACACCCTGATCCGCATCATGGTCTCC
  CGTAGTGAGTTGGACATGCTCGACATTCGGGAGATCTTCCGGACCAAGTATGAGAAGTCC
  CTCTACAGCATGATCAAGAATGACACCTCTGGCGAGTACAAGAAGACTCTGCTGAAGCTG
  TCTGGGGGAGATGATGATGCTGCTGGCCAGTTCTTCCCGGAGGCAGCGCAGGTGGCCTAT
  CAGATGTGGGAACTTAGTGCAGTGGCCCGAGTAGAGCTGAAGGGAACTGTGCGCCCAGCC
  AATGACTTCAACCCTGACGCAGATGCCAAAGCGCTGCGGAAAGCCATGAAGGGACTCGGG
  ACTGACGAAGACACAATCATCGATATCATCACGCACCGCAGCAATGTCCAGCGGCAGCAG
  ATCCGGCAGACCTTCAAGTCTCACTTTGGCCGGGACTTAATGACTGACCTGAAGTCTGAG
  ATCTCTGGAGACCTGGCAAGGCTGATTCTGGGGCTCATGATGCCACCGGCCCATTACGAT
  GCCAAGCAGTTGAAGAAGGCCATGGAGGGAGCCGGCACAGATGAAAAGGCTCTTATTGAA
  ATCCTGGCCACTCGGACCAATGCTGAAATCCGGGCCATCAATGAGGCCTATAAGGAGGAC
  TATCACAAGTCCCTGGAGGATGCTCTGAGCTCAGACACATCTGGCCACTTCAGGAGGATC
  CTCATTTCTCTGGCCACGGGGCATCGTGAGGAGGGAGGAGAAAACCTGGACCAGGCACGG
  GAAGATGCCCAGGTGGCTGCTGAGATCTTGGAAATAGCAGACACACCCAGTGGAGACAAA
  ACTTCCTTGGAGACACGTTTCATGACGATCCTGTGTACCCGGAGCTATCCGCACCTCCGG
  AGAGTCTTCCAGGAGTTCATCAAGATGACCAACTATGACGTGGAGCACACCATCAAGAAG
  GAGATGTCTGGGGATGTCAGGGATGCATTTGTGGCCATTGTTCAAAGTGTCAAGAACAAG
  CCTCTCTTCTTTGCCGACAAACTTTACAAATCCATGAAGGGTGCTGGCACAGATGAGAAG
  ACTCTGACCAGGATCATGGTATCCCGCAGTGAGATTGACCTGCTCAACATCCGGAGGGAA
  TTCATTGAGAAATATGACAAGTCTCTCCACCAAGCCATTGAGGGTGACACCTCCGGAGAC
  TTCCTGAAGGCCTTGCTGGCTCTCTGTGGTGGTGAGGACTAG
  
  Clone variation with respect to NM_001155.4
  1608 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001155 unedited
  AGAATTGTATACGACTACTATAGGCGGCCCGCGAATTCGCACGAGGCCTCGTGCCGAATT
  CGGCACGAGGGCGAACCGGAGACCATGGCCAAACCAGCACAGGGTGCCAAGTACCGGGGC
  TCCATCCATGACTTCCCAGGCTTTGACCCCAACCAGGATGCCGAGGCTCTGTACACTGCC
  ATGAAGGGCTTTGGCAGTGACAAGGAGGCCATACTGGACATAATCACCTCACGGAGCAAC
  AGGCAGAGGCAGGAGGTCTGCCAGAGCTACAAGTCCCTCTACGGCAAGGACCTCATTGCT
  GATTTAAAGTATGAATTGACGGGCAAGTTTGAACGCTTGATTGTGGGCCTGATGAGGCCA
  CCTGCCTATTGTGATGCCAAAGAAATTAAAGATGCCATCTCGGGCATTGGCACTGATGAG
  AAGTGCCTCATTGAGATCTTGGCTTCCCGGACCAATGAGCAGATGCACCAGCTGGTGGCA
  GCATACAAAGATGCCTACGAGCGGGACCTGCAGGCTGACATCATCGGCGACACCTCTGGC
  CACTTCCAGAAGATGCTTGTGGTCCTGCTCCAGGGAACCAGGGAGGAGGATGACGTATTG
  AGCGAGGACCTGGTACAACAGGATGCCCAGGACCTATACGAGGCAAGGGAACTGAAATGG
  GGAACAGATGAAGCCCAGTTCATTTACATCTTGGGAAATCGCAGCAAGCACCATCTTCGG
  TTGGTGTTCGATGAGTATCTGAAGACACAGGGAAGCCGCATGAAGCCACCTCCCGAGGGA
  GCTGTCTGGGCACTTTGACACCTAATGCTTGCCGTACGAAATGTATCCCCAGCACCCCGC
  ATATTCTGCTGAAAGGCTCTTCCAGCCTATGAAGGGCCTGGGGACTCGGGACACACCCTG
  AACCCATCATGGCCCCCGTAAGGAACTGCCATGCTCCACATCCGAAAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001155 unedited
  TGACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGAC
  AGAGGTTCAGGATGTTTTTGGATCTGACATAGCCCAAACCAGGGCAGAGGCTGTAGCAAG
  GGGAGGAAGCTGGGAAAGCCTGAGGGTCAAAGGGGAGTGGAGGTGGACAGGATCTATGGC
  TACGGTTTTTCAGCCGCTCAGCCGTGCTGGGCCCTCGATGGCCCGTGGGAGTGGGAGCGT
  TTCCTAAGCTCCACTGAAGATAAGAGCCCAACCCAACCCCTCCCCCCACCCCTGCCCCTT
  CCTTAGTCTCTGGAGCTGGAACAATCAGGCTTGGCCATGGCGGCTGGTGCTGATAACCAT
  TTCTTGGCAGAAGTGCCCGCCAAAGCTGTGGCCCTAGTCCTCACCACCACAGAGAGCCAG
  CAAGGCCTTCAGGAAGTCTCCGGAGGTGTCACCCTCAATGGCTTGGTGGAGAGACTTGTC
  ATATTTCTCAATGAATTCCCTCCGGATGTTGAGCAGGTCAATCTCACTGCGGGATACCAT
  GATCCTGGTCAGAGTCTTCTCATCTGTGCCAGCACCCTTCATGGATTTGTAAAGTTTGTC
  GGCAAAGAAGAGAGGCTTGTTCTTGACACTTTGAACAATGGCCACAAATGCATCCCTGAC
  ATCCCCAGACATCTCCTTCTTGATGGTGTGCTCCCCGTCATAATTGGTCATCTTGATGAA
  CTCCTGGAAGACTCTTCCGAGTGCCGATAGCTCCCGGTACACAGATCGTCATGAAACGTG
  TCTCCCAGGAAGTTTGCCTCACTGGGTGTGTCTGCTATTTCCAGGACTTAGCAGCCACTG
  GGCATNTTCCCGGCCTGGTCCAGTTTTCTCCTCCCTCTCACAAGCCCCGGGCCAAAGAAT
  GAAGATCCTTCTAAGGGGCCATATGTGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001155
• Insert Size: 2720 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001155.3, NP_001146.2
• RefSeq Size: 2915 bp
• RefSeq ORF: 2022 bp
• Locus ID: 309
• Cytogenetics: 5q33.1
• Domains: annexin
• Gene Summary: 'Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).