alpha Actinin (ACTN1) (NM_001102) Human Untagged Clone

CAT#: SC119433

ACTN1 (untagged)-Human actinin, alpha 1 (ACTN1), transcript variant 2


  "NM_001102" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ACTN1
Synonyms BDPLT15
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119433 sequence for NM_001102 edited (data generated by NextGen Sequencing)
ATGGACCATTATGATTCTCAGCAAACCAACGATTACATGCAGCCAGAAGAGGACTGGGAC
CGGGACCTGCTCCTGGACCCGGCCTGGGAGAAGCAGCAGAGAAAGACATTCACGGCATGG
TGTAACTCCCACCTCCGGAAGGCGGGGACACAGATCGAGAACATCGAAGAGGACTTCCGG
GATGGCCTGAAGCTCATGCTGCTGCTGGAGGTCATCTCAGGTGAACGCTTGGCCAAGCCA
GAGCGAGGCAAGATGAGAGTGCACAAGATCTCCAACGTCAACAAGGCCCTGGATTTCATA
GCCAGCAAAGGCGTCAAACTGGTGTCCATCGGAGCCGAAGAAATCGTGGATGGGAATGTG
AAGATGACCCTGGGCATGATCTGGACCATCATCCTGCGCTTTGCCATCCAGGACATCTCC
GTGGAAGAGACTTCAGCCAAGGAAGGGCTGCTCCTGTGGTGTCAGAGAAAGACAGCCCCT
TACAAAAATGTCAACATCCAGAACTTCCACATAAGCTGGAAGGATGGCCTCGGCTTCTGT
GCTTTGATCCACCGACACCGGCCCGAGCTGATTGACTACGGGAAGCTGCGGAAGGATGAT
CCACTCACAAATCTGAATACGGCTTTTGACGTGGCAGAGAAGTACCTGGACATCCCCAAG
ATGCTGGATGCCGAAGACATCGTTGGAACTGCCCGACCGGATGAGAAAGCCATCATGACT
TACGTGTCTAGCTTCTACCACGCCTTCTCTGGAGCCCAGAAGGCGGAGACAGCAGCCAAT
CGCATCTGCAAGGTGTTGGCCGTCAACCAGGAGAACGAGCAGCTTATGGAAGACTACGAG
AAGCTGGCCAGTGATCTGTTGGAGTGGATCCGCCGCACAATCCCGTGGCTGGAGAACCGG
GTGCCCGAGAACACCATGCATGCCATGCAACAGAAGCTGGAGGACTTCCGGGACTACCGG
CGCCTGCACAAGCCGCCCAAGGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACG
CTGCAGACCAAGCTGCGGCTCAGCAACCGGCCTGCCTTCATGCCCTCTGAGGGCAGGATG
GTCTCGGACATCAACAATGCCTGGGGCTGCCTGGAGCAGGTGGAGAAGGGCTATGAGGAG
TGGTTGCTGAATGAGATCCGGAGGCTGGAGCGACTGGACCACCTGGCAGAGAAGTTCCGG
CAGAAGGCCTCCATCCACGAGGCCTGGACTGACGGCAAAGAGGCCATGCTGCGACAGAAG
GACTATGAGACCGCCACCCTCTCGGAGATCAAGGCCTTGCTCAAGAAGCATGAGGCCTTC
GAGAGTGACCTGGCTGCCCACCAGGACCGTGTGGAGCAGATTGCCGCCATCGCACAGGAG
CTCAATGAGCTGGACTATTATGACTCACCCAGTGTCAACGCCCGTTGCCAAAAGATCTGT
GACCAGTGGGACAATCTGGGGGCCCTAACTCAGAAGCGAAGGGAAGCTCTGGAGCGGACC
GAGAAACTGCTGGAGACCATTGACCAGCTGTACTTGGAGTATGCCAAGCGGGCTGCACCC
TTCAACAACTGGATGGAGGGGGCCATGGAGGACCTGCAGGACACCTTCATTGTGCACACC
ATTGAGGAGATCCAGGGACTGACCACAGCCCATGAGCAGTTCAAGGCCACCCTCCCTGAT
GCCGACAAGGAGCGCCTGGCCATCCTGGGCATCCACAATGAGGTGTCCAAGATTGTCCAG
ACCTACCACGTCAATATGGCGGGCACCAACCCCTACACAACCATCACGCCTCAGGAGATC
AATGGCAAATGGGACCACGTGCGGCAGCTGGTGCCTCGGAGGGACCAAGCTCTGACGGAG
GAGCATGCCCGACAGCAGCACAATGAGAGGCTACGCAAGCAGTTTGGAGCCCAGGCCAAT
GTCATCGGGCCCTGGATCCAGACCAAGATGGAGGAGATCGGGAGGATCTCCATTGAGATG
CATGGGACCCTGGAGGACCAGCTCAGCCACCTGCGGCAGTATGAGAAGAGCATCGTCAAC
TACAAGCCAAAGATTGATCAGCTGGAGGGCGACCACCAGCTCATCCAGGAGGCGCTCATC
TTCGACAACAAGCACACCAACTACACCATGGAGCACATCCGTGTGGGCTGGGAGCAGCTG
CTCACCACCATCGCCAGGACCATCAATGAGGTAGAGAACCAGATCCTGACCCGGGATGCC
AAGGGCATCAGCCAGGAGCAGATGAATGAGTTCCGGGCCTCCTTCAACCACTTTGACCGG
GATCACTCCGGCACACTGGGTCCCGAGGAGTTCAAAGCCTGCCTCATCAGCTTGGGTTAT
GATATTGGCAACGACCCCCAGGGAGAAGCAGAATTTGCCCGCATCATGAGCATTGTGGAC
CCCAACCGCCTGGGGGTAGTGACATTCCAGGCCTTCATTGACTTCATGTCCCGCGAGACA
GCCGACACAGATACAGCAGACCAAGTCATGGCTTCCTTCAAGATCCTGGCTGGGGACAAG
AACTACATTACCATGGACGAGCTGCGCCGCGAGCTGCCACCCGACCAGGCTGAGTACTGC
ATCGCGCGGATGGCCCCCTACACCGGCCCCGACTCCGTGCCAGGTGCTCTGGACTACATG
TCCTTCTCCACGGCGCTGTACGGCGAGAGTGACCTCTAA

Clone variation with respect to NM_001102.3
1297 c=>t
>OriGene 5' read for NM_001102 unedited
NGGTTTAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTAGTTC
AGTGTGCGTGGAGATTAGGTCCAAGCGCCCGCCCAGAGGCGGCAGTCCGCGAGCCCAGCC
GCCGCTGTCGCCGCCAGTAGCAGCCTTCGCCAGCAGCGCCGCGGCGGAACCGGGCGCAGG
GGAGCGAGCCCGGCCCCGCCAGCCCAGCCCAGCCCAGCCCAGCCCTACTCCCTCCCCACG
CCAGGGCAGCAGCCGTTGCTCAGAGAGAAGGTGGAGGAAGAAATCCAGACCCTAGCACGC
GCGCACCATCATGGACCATTATGATTCTCAGCAAACCAACGATTACATGCAGCCAGAAGA
GGACTGGGACCGGGACCTGCTCCTGGACCCGGCCTGGGAGAAGCAGCAGAGAAAGACATT
CACGGCATGGTGTAACTCCCACCTCCGGAAGGCGGGGACACAGATCGAGAACATCGAAGA
GGACTTCCGGGATGGCCTGAAGCTCATGCTGCTGCTGGAGGTCATCTCAGGTGAACGCTT
GGCCAAGCCAGAGCGAGGCAAGATGAGAGTGCACAAGATCTCCAACGTCAACAAGGCCCT
GGATTTCATAGCCAGCAAAGGCGTCAAACTGGTGTCCATCGGAGCCCGAAGAAATCGTGG
ATTGGGGAATGTGAAGATGACCCTGTGCATGACCTGGCCATCATCCTGCGCTTTGGCATC
CAGGACATCTCCGTGGAAGAGACTTCAGCCAAGGAAGGCCTGCTCCTGTGGTGTCAGAGC
AAGACAGCCCCTTACAAAATGTAAACCTTCAGAACTCTCACATAGCTGGAAGGATGGCCT
CGCTTCTGTGCTTTGATCCACCGACACCGGCCCCACCTGTATTGACTCGGTAAGCTGCGG
AAG
>OriGene 3' read for NM_001102 unedited
CGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTCTAGGAGTGTGGTTTT
TTTAATACCATCTGTGCCAAGCAATATCAAAGTTGTCCATACTCTCCAAGTTTGTCCTAT
TTTTAATAGACAATATTAAAAATTTTTTAAGTGTGTGCTGATAAAGAGACTAGTCACACC
TCTGAGGGGATTTAAAAATGAGAAGCCACATAAATTCTCTTCCTTAAAAAACAGCCATTT
CAAAAGATTTTCTTTAATATCATAAAATATTTTCATGGACAAGTGAGCTAGCAAACACAC
ATGCACCAATGTGCCTTTTGACAAGAGTACCCCCTACCCCGACTCCCACACCAAAATGGA
CATGAGATTGGAGAAATGAATACAGCAGATGGAACAGATAGAAGAAAAAAAATCAGTAAA
AAGGATGGAATATAACTTTGTGCTTGGTTATTTTCCTATGTCGCAACAAAACAAGACATT
TCGGTGCAAATAGTTAATCTTTCCTCTTTTTTTCCATTTGTCTGGTGGAGAAAAAATACT
TTTTCTATAATAAAATATGTAGTTTTTTGGTTTTTAACGTAACTTTTTTTTTCTTTTTTG
CAGAAAATAATTTTGTAAACTGTCACTTCGCGGGCAGGGAGGATCGATGCCACGTGGGCC
CCAGCTCACCCGGGTGGAGGCTGGGAGCTGAAACCGAACCCAGCAGGATATGGGCGACGG
CGGATGTGCAAGGCATGGCACGGCGCACAATACNAGGGCGGCCGGGCGGGGTGGATTAAA
GGTCACTCTCGCCGTACAGCGCCGTGGAGATGACATGTANTCCATAACACCCTGCACGGA
GTCGGGGCCGTGTTAGGGGCCCATCCCGCGCAGCAGTACTCANCCTGTTTCGGTGGCACC
TCGCGGCGCAACTCGNCCATGGTAATGTAANTCCTTGCCCCCAGCAAGATCTTGTAGGNA
AGCATGACTGTGGCTGCTGTN
Restriction Sites NotI-NotI     
ACCN NM_001102
Insert Size 3520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001102.2, NP_001093.1
RefSeq Size 3398 bp
RefSeq ORF 2679 bp
Locus ID 87
Cytogenetics 14q22-q24
Domains CH, spectrin, EFh
Protein Families Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
Gene Summary 'Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

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