ADAM10 (NM_001110) Human Untagged Clone

CAT#: SC119437

ADAM10 (untagged)-Human ADAM metallopeptidase domain 10 (ADAM10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAM10
• Synonyms: AD10; AD18; CD156c; CDw156; HsT18717; kuz; MADM; RAK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001110 edited
  GCGAATTCGGCACGAGGCCGTTTCTCCTGCCAGGGGAGGTCCCGGCTTCCCGTGGAGGCT
  CCGGACCAAGCCCCTTCAGCTTCTCCCTCCGGATCGATGTGCTGCTGTTAACCCGTGAGG
  AGGCGGCGGCGGCGGCAGCGGCAGCGGAAGATGGTGTTGCTGAGAGTGTTAATTCTGCTC
  CTCTCCTGGGCGGCGGGGATGGGAGGTCAGTATGGGAATCCTTTAAATAAATATATCAGA
  CATTATGAAGGATTATCTTACAATGTGGATTCATTACACCAAAAACACCAGCGTGCCAAA
  AGAGCAGTCTCACATGAAGACCAATTTTTACGTCTAGATTTCCATGCCCATGGAAGACAT
  TTCAACCTACGAATGAAGAGGGACACTTCCCTTTTCAGTGATGAATTTAAAGTAGAAACA
  TCAAATAAAGTACTTGATTATGATACCTCTCATATTTACACTGGACATATTTATGGTGAA
  GAAGGAAGTTTTAGCCATGGGTCTGTTATTGATGGAAGATTTGAAGGATTCATCCAGACT
  CGTGGTGGCACATTTTATGTTGAGCCAGCAGAGAGATATATTAAAGACCGAACTCTGCCA
  TTTCACTCTGTCATTTATCATGAAGATGATATTAACTATCCCCATAAATACGGTCCTCAG
  GGGGGCTGTGCAGATCATTCAGTATTTGAAAGAATGAGGAAATACCAGATGACTGGTGTA
  GAGGAAGTAACACAGATACCTCAAGAAGAACATGCTGCTAATGGTCCAGAACTTCTGAGG
  AAAAAACGTACAACTTCAGCTGAAAAAAATACTTGTCAGCTTTATATTCAGACTGATCAT
  TTGTTCTTTAAATATTACGGAACACGAGAAGCTGTGATTGCCCAGATATCCAGTCATGTT
  AAAGCGATTGATACAATTTACCAGACCACAGACTTCTCCGGAATCCGTAACATCAGTTTC
  ATGGTGAAACGCATAAGAATCAATACAACTGCTGATGAGAAGGACCCTACAAATCCTTTC
  CGTTTCCCAAATATTGGTGTGGAGAAGTTTCTGGAATTGAATTCTGAGCAGAATCATGAT
  GACTACTGTTTGGCCTATGTCTTCACAGACCGAGATTTTGATGATGGCGTACTTGGTCTG
  GCTTGGGTTGGAGCACCTTCAGGAAGCTCTGGAGGAATATGTGAAAAAAGTAAACTCTAT
  TCAGATGGTAAGAAGAAGTCCTTAAACACTGGAATTATTACTGTTCAGAACTATGGGTCT
  CATGTACCTCCCAAAGTCTCTCACATTACTTTTGCTCACGAAGTTGGACATAACTTTGGA
  TCCCCACATGATTCTGGAACAGAGTGCACACCAGGAGAATCTAAGAATTTGGGTCAAAAA
  GAAAATGGCAATTACATCATGTATGCAAGAGCAACATCTGGGGACAAACTTAACAACAAT
  AAATTCTCACTCTGTAGTATTAGAAATATAAGCCAAGTTCTTGAGAAGAAGAGAAACAAC
  TGTTTTGTTGAATCTGGCCAACCTATTTGTGGAAATGGAATGGTAGAACAAGGTGAAGAA
  TGTGATTGTGGCTATAGTGACCAGTGTAAAGATGAATGCTGCTTCGATGCAAATCAACCA
  GAGGGAAGAAAATGCAAACTGAAACCTGGGAAACAGTGCAGTCCAAGTCAAGGTCCTTGT
  TGTACAGCACAGTGTGCATTCAAGTCAAAGTCTGAGAAGTGTCGGGATGATTCAGACTGT
  GCAAGGGAAGGAATATGTAATGGCTTCACAGCTCTCTGCCCAGCATCTGACCCTAAACCA
  AACTTCACAGACTGTAATAGGCATACACAAGTGTGCATTAATGGGCAATGTGCAGGTTCT
  ATCTGTGAGAAATATGGCTTAGAGGAGTGTACGTGTGCCAGTTCTGATGGCAAAGATGAT
  AAAGAATTATGCCATGTATGCTGTATGAAGAAAATGGACCCATCAACTTGTGCCAGTACA
  GGGTCTGTGCAGTGGAGTAGGCACTTCAGTGGTCGAACCATCACCCTGCAACCTGGATCC
  CCTTGCAACGATTTTAGAGGTTACTGTGATGTTTTCATGCGGTGCAGATTAGTAGATGCT
  GATGGTCCTCTAGCTAGGCTTAAAAAAGCAATTTTTAGTCCAGAGCTCTATGAAAACATT
  GCTGAATGGATTGTGGCTCATTGGTGGGCAGTATTACTTATGGGAATTGCTCTGATCATG
  CTAATGGCTGGATTTATTAAGATATGCAGTGTTCATACTCCAAGTAGTAATCCAAAGTTG
  CCTCCTCCTAAACCACTTCCAGGCACTTTAAAGAGGAGGAGACCTCCACAGCCCATTCAG
  CAACCCCAGCGTCAGCGGCCCCGAGAGAGTTATCAAATGGGACACATGAGACGCTAACTG
  CAGCTTTTGCCTTGGTTCTTCCTAGTGCCTACAATGGGAAAACTTCACTCCAAAGAGAAA
  CCTATTAAGTCATCATCTCCAAACTAAACCCTCACAAGTAACAGTTGAAGAAAAAATGGC
  AAGAGATCATATCCTCAGACCAGGTGGAATTACTTAAATTTTAAAGCCTGAAAATTCCAA
  TTTGGGGGTGGGAGGTGGAAAAGGAACCCAATTTTCTTATGAACAGATATTTTTAACTTA
  ATGGCACAAAGTCTTAGAATATTATTATGTGCCCCGTGTTCCCTGTTCTTCGTTGCTGCA
  TTTTCTTCACTTGCAGGCAAACTTGGCTCTCAATAAACTTTTACCACAAATTGAAATAAA
  TATATTTTTTTCAACTGCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001110 unedited
  NGGTTCAAATTTCGGATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCGTTTC
  TCCTGCCAGGGAGGTCCCGGCTTCCCGTGGAGGCTCCGGACCAAGCCCCTTCAGCTTCTC
  CCTCCGGATCGATGTGCTGCTGTTAACCCGTGAGGAGGCGGCGGCGGCGGCAGCGGCAGC
  GGAAGATGGTGTTGCTGAGAGTGTTAATTCTGCTCCTCTCCTGGGCGGCGGGGATGGGAG
  GTCAGTATGGGAATCCTTTAAATAAATATATCAGACATTATGAAGGATTATCTTACAATG
  TGGATTCATTACACCAAAAACACCAGCGTGCCAAAAGAGCAGTCTCACATGAAGACCAAT
  TTTTACGTCTAGATTTCCATGCCCATGGAAGACATTTCAACCTACGAATGAAGAGGGACA
  CTTCCCTTTTCAGTGATGAATTTAAAGTAGAAACATCAAATAAAGTACTTGATTATGATA
  CCTCTCATATTTACACTGGACATATTTATGGTGAAGAAGGAAGTTTTAGCCATGGGTCTG
  TTATTGATGGAAGATTTGAAGGATTCATCCAGACTCGTGGTGGCACATTTTATGTTGAGC
  CAGCAGAGAGATATATTAAAGACCGAACTCTGCCATTTCACTCTGTCATTTATCATGAAG
  ATGATATTAACTATCCCCATAAATACGGTCCTCANGGGGGCTGTGCAGATCATTCAGTAT
  TTGAAAGAATGAGGAAATACCAGATGACTGGTGTAGAGGAAAGTACACAGATACCTCAAG
  AAGACATGCTGCNTATGGTCCAGAACTTNCTGAGAAAAACGTACACTTCAGCTGAAAAAA
  TACTTGTCAGCTNTATATTCAGACTGATCATTTGTTCCTTTAATATTACGGGACACCGAA
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001110
• Insert Size: 4760 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110.2, NP_001101.1
• RefSeq Size: 3927 bp
• RefSeq ORF: 2247 bp
• Locus ID: 102
• Cytogenetics: 15q21.3
• Domains: Reprolysin, DISIN
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection
• Gene Summary: 'Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).