ADAR1 (ADAR) (NM_001111) Human Untagged Clone

CAT#: SC119438

ADAR (untagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1


  "NM_001111" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ADAR
Synonyms ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119438 sequence for NM_001111 edited (data generated by NextGen Sequencing)
ATGAATCCGCGGCAGGGGTATTCCCTCAGCGGATACTACACCCATCCATTTCAAGGCTAT
GAGCACAGACAGCTCAGATACCAGCAGCCTGGGCCAGGATCTTCCCCCAGTAGTTTCCTG
CTTAAGCAAATAGAATTTCTCAAGGGGCAGCTCCCAGAAGCACCGGTGATTGGAAAGCAG
ACACCGTCACTGCCACCTTCCCTCCCAGGACTCCGGCCAAGGTTTCCAGTACTACTTGCC
TCCAGTACCAGAGGCAGGCAAGTGGACATCAGGGGTGTCCCCAGGGGCGTGCATCTCGGA
AGTCAGGGGCTCCAGAGAGGGTTCCAGCATCCTTCACCACGTGGCAGGAGTCTGCCACAG
AGAGGTGTTGATTGCCTTTCCTCACATTTCCAGGAACTGAGTATCTACCAAGATCAGGAA
CAAAGGATCTTAAAGTTCCTGGAAGAGCTTGGGGAAGGGAAGGCCACCACAGCACATGAT
CTGTCTGGGAAACTTGGGACTCCGAAGAAAGAAATCAATCGAGTTTTATACTCCCTGGCA
AAGAAGGGCAAGCTACAGAAAGAGGCAGGAACACCCCCTTTGTGGAAAATCGCGGTCTCC
ACTCAGGCTTGGAACCAGCACAGCGGAGTGGTAAGACCAGACGGTCATAGCCAAGGAGCC
CCAAACTCAGACCCGAGTTTGGAACCGGAAGACAGAAACTCCACATCTGTCTCAGAAGAT
CTTCTTGAGCCTTTTATTGCAGTCTCAGCTCAGGCTTGGAACCAGCACAGCGGAGTGGTA
AGACCAGACAGTCATAGCCAAGGATCCCCAAACTCAGACCCAGGTTTGGAACCTGAAGAC
AGCAACTCCACATCTGCCTTGGAAGATCCTCTTGAGTTTTTAGACATGGCCGAGATCAAG
GAGAAAATCTGCGACTATCTCTTCAATGTGTCTGACTCCTCTGCCCTGAATTTGGCTAAA
AATATTGGCCTTACCAAGGCCCGAGATATAAATGCTGTGCTAATTGACATGGAAAGGCAG
GGGGATGTCTATAGACAAGGGACAACCCCTCCCATATGGCATTTGACAGACAAGAAGCGA
GAGAGGATGCAAATCAAGAGAAATACGAACAGTGTTCCTGAAACCGCTCCAGCTGCAATC
CCTGAGACCARAAGAAACGCAGAGTTCCTCACCTGTAATATACCCACATCAAATGCCTCA
AATAACATGGTAACCACAGAAAAAGTGGAGAATGGGCAGGAACCTGTCATAAAGTTAGAA
AACAGGCAAGAGGCCAGACCAGAACCAGCAAGACTGAAACCACCTGTTCATTACAATGGC
CCCTCAAAAGCAGGGTATGTTGACTTTGAAAATGGCCAGTGGGCCACAGATGACATCCCA
GATGACTTGAATAGTATCCGCGCAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCC
TCCTTCTACAGTCATGGCTTGCCACGGTGTTCACCCTACAAGAAACTGACAGAGTGCCAG
CTGAAGAACCCCATCAGCGGGCTGTTAGAATATGCCCAGTTCGCTAGTCAAACCTGTGAG
TTCAACATGATAGAGCAGAGTGGACCACCCCATGAACCTCGATTTAAATTCCAGGTTGTC
ATCAATGGCCGAGAGTTTCCCCCAGCTGAAGCTGGAAGCAAGAAAGTGGCCAAGCAGGAT
GCAGCTATGAAAGCCATGACAATTCTGCTAGAGGAAGCCAAAGCCAAGGACAGTGGAAAA
TCAGAAGAATCATCCCACTATTCCACAGAGAAAGAATCAGAGAAGACTGCAGAGTCCCAG
ACCCCCACCCCTTCAGCCACATCCTTCTTTTCTGGGAAGAGCCCCGTCACCACACTGCTT
GAGTGTATGCACAAATTGGGGAACTCCTGCGAATTCCGTCTCCTGTCCAAAGAAGGCCCT
GCCCATGAACCCAAGTTCCAATACTGTGTTGCAGTGGGAGCCCAAACTTTCCCCAGTGTG
AGTGCTCCCAGCAAGAAAGTGGCAAAGCAGATGGCCGCAGAGGAAGCCATGAAGGCCCTG
CATGGGGAGGCGACCAACTCCATGGCTTCTGATAACCAGCCTGAAGGTATGATCTCAGAG
TCACTTGATAACTTGGAATCCATGATGCCCAACAAGGTCAGGAAGATTGGCGAGCTCGTG
AGATACCTGAACACCAACCCTGTGGGTGGCCTTTTGGAGTACGCCCGCTCCCATGGCTTT
GCTGCTGAATTCAAGTTGGTCGACCAGTCCGGACCTCCTCACGAGCCCAAGTTCGTTTAC
CAAGCAAAAGTTGGGGGTCGCTGGTTCCCAGCCGTCTGCGCACACAGCAAGAAGCAAGGC
AAGCAGGAAGCAGCAGATGCGGCTCTCCGTGTCTTGATTGGGGAGAACGAGAAGGCAGAA
CGCATGGGTTTCACAGAGGTAACCCCAGTGACAGGGGCCAGTCTCAGAAGAACTATGCTC
CTCCTCTCAAGGTCCCCAGAAGCACAGCCAAAGACACTCCCTCTCACTGGCAGCACCTTC
CATGACCAGATAGCCATGCTGAGCCACCGGTGCTTCAACACTCTGACTAACAGCTTCCAG
CCCTCCTTGCTCGGCCGCAAGATTCTGGCCGCCATCATTATGAAAAAAGACTCTGAGGAC
ATGGGTGTCGTCGTCAGCTTGGGAACAGGGAATCGCTGTGTGAAAGGAGATTCTCTCAGC
CTAAAAGGAGAAACTGTCAATGACTGCCATGCAGAAATAATCTCCCGGAGAGGCTTCATC
AGGTTTCTCTACAGTGAGTTAATGAAATACAACTCCCAGACTGCGAAGGATAGTATATTT
GAACCTGCTAAGGGAGGAGAAAAGCTCCAAATAAAAAAGACTGTGTCATTCCATCTGTAT
ATCAGCACTGCTCCGTGTGGAGATGGCGCCCTCTTTGACAAGTCCTGCAGCGACCGTGCT
ATGGAAAGCACAGAATCCCGCCACTACCCTGTCTTCGAGAATCCCAAACAAGGAAAGCTC
CGCACCAAGGTGGAGAACGGAGAAGGCACAATCCCTGTGGAATCCAGTGACATTGTGCCT
ACGTGGGATGGCATTCGGCTCGGGGAGAGACTCCGTACCATGTCCTGTAGTGACAAAATC
CTACGCTGGAACGTGCTGGGCCTGCAAGGGGCACTGTTGACCCACTTCCTGCAGCCCATT
TATCTCAAATCTGTCACATTGGGTTACCTTTTCAGCCAAGGGCATCTGACCCGTGCTATT
TGCTGTCGTGTGACAAGAGATGGGAGTGCATTTGAGGATGGACTACGACATCCCTTTATT
GTCAACCACCCCAAGGTTGGCAGAGTCAGCATATATGATTCCAAAAGGCAATCCGGGAAG
ACTAAGGAGACAAGCGTCAACTGGTGTCTGGCTGATGGCTATGACCTGGAGATCCTGGAC
GGTACCAGAGGCACTGTGGATGGGCCACGGAATGAATTGTCCCGGGTCTCCAAAAAGAAC
ATTTTTCTTCTATTTAAGAAGCTCTGCTCCTTCCGTTACCGCAGGGATCTACTGAGACTC
TCCTATGGTGAGGCCAAGAAAGCTGCCCGTGACTACGAGACGGCCAAGAACTACTTCAAA
AAAGGCCTGAAGGATATGGGCTATGGGAACTGGATTAGCAAACCCCAGGAGGAAAAGAAC
TTTTATCTCTGCCCAGTATAG

Clone variation with respect to NM_001111.4
78 g=>a;1151 a=>r
>OriGene 5' read for NM_001111 unedited
TAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
AATTCGGCACGAGGCTTGGGCCCGGCGCGCAGACCCGCGGAGTTTCCCGTGCCGACGCCC
CGGGGCCACTTCCAGTGCGGAGTAGCGGAGGCGTGGGGGCCTCGAGGGGCTGGCGCGGCC
CAGCGGTCGGGCCAGGGTCGTGCCGCCGGCGGGTCGGGCCGGGCAATGCCTCGCGGGCGC
AATGAATCCGCGGCAGGGGTATTCCCTCAGCGGATACTACACCCATCCATTTCAAGGCTA
TGAGCACAGACAGCTCAGATACCAGCAGCCTGGGCCAGGATCTTCCCCCAGTAGTTTCCT
GCTTAAGCAAATAGAATTTCTCAAGGGGCAGCTCCCAGAAGCACCGGTGATTGGAAAGCA
GACACCGTCACTGCCACCTTCCCTCCCAGGACTCCGGCCAAGGTTTCCAGTACTACTTGC
CTCCAGTACCAGAGGCAGGNCAGTGGACATCAGGGGTGTCCCCAGGGGCGTGCATCTCGG
AAGTCAGGGGCTCCAGAGAGGGTTNCAGCATCCTTCACCACGTGGCAGGAGTCTGCCCAG
AGAGGTGTTGATTGCCTTTTCTCACATTNCCAGGACTGAGTATCTACAAGATCAGGACAN
AGGATCCTTAAGTTCCTGGAANAGCCTGGGGAAAGGAAGGGCANCACCGCACATGATCTG
TCTGGGAAACTTGGGACTCCGAGAAGAAGTCAATCGAGTTTATACTCCTGGCAAAGAAGG
CAGCTCAGAAGAGGCAGAACCCCCTTGTGAAAAAACGGGCTCCCTCAGCTTGGACCCAGC
GGGGTGGGTAAGACCAACGGCTAGCCAGGGCCCACTAACCCCCGTTTGGACCGGGAA
>OriGene 3' read for NM_001111 unedited
GGTTCCACTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTCAA
ATTCTTGTTTTTTTTTTATTTATGAGGAATGTATATTGTTAAGTCACTGTTATCAAGGGA
CACATAAAAGCAGAATCATAAAACTGGACTGCAGCCATCATCACAGTACCCGAGTCTATG
CTTGGGGGTCTTCCTCACTCTGCTCTTGGAGTCACAACCAAGACTCCAAAAAACCAACGA
AGTCCCTTCAATGTGAGTAAAGGAAACAGCTTCAAGCACTGACAATTTTTACAAGTGATT
ATTCAAGGAATGCACAGTAGCCACAGTTCAGACAAATCAACTACAAAAATCATTAAAAAG
AAGAGTAGGGAGGGTCACTGCTTATTAAAAACAAAAGACTGGACAAAGAAAGTGAAGATA
ACAGTGGGCAGAAGCTCTGCCTCCTCTGTCCTGTGTGACTCACAGGGGCATGGGGGCATG
GGGGCCCAAGGATGTGCGATCTGACACGGCTCGTTTCCAACAGATGCGTGGAATTCAAGG
ATGCCTCGCTCTCTTCCTACAACAGAAACTGAATACTACAGGCTTGCTAAATAAAATCTT
GGGGGGGAAGGGGGGGTTCTGAACTTGTGAAAACCCGTGTTCCCCGTTAAAAAAAAGCAA
CTGGCCCAAACTCTGCTGGAAAACTCATGCCCCAGATGGACCCAAAGGCCAAACACCCGA
GGACTCAGCGGGGACAACCCTGTGCCCTAAAAAAAAAGGGGTGCCCCTCCTTCTTATGCG
GAAAACCCAAAACCTTTTTGCCCGGAATTGGGTGCCCAAAATGTTTTATGTTGCCCCCCA
TAAACCCAAGCCGGGGAGAGGGCTTTTTCACTTTTGGAAAAGGGGCCCTTTTCTACCATT
Restriction Sites NotI-NotI     
ACCN NM_001111
Insert Size 7000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001111.3, NP_001102.2
RefSeq Size 6640 bp
RefSeq ORF 3681 bp
Locus ID 103
Cytogenetics 1q21.3
Domains z-alpha, DSRM, A_deamin
Protein Families Druggable Genome
Protein Pathways Cytosolic DNA-sensing pathway
Gene Summary 'This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]'
Transcript Variant: This variant (1) encodes isoform (a, also referred to as ADAR-a).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.