VCAM1 (NM_001078) Human Untagged Clone

CAT#: SC119467

VCAM1 (untagged)-Human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1


  "NM_001078" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol VCAM1
Synonyms CD106; INCAM-100
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119467 sequence for NM_001078 edited (data generated by NextGen Sequencing)
ATGCCTGGGAAGATGGTCGTGATCCTTGGAGCCTCAAATATACTTTGGATAATGTTTGCA
GCTTCTCAAGCTTTTAAAATCGAGACCACCCCAGAATCTAGATATCTTGCTCAGATTGGT
GACTCCGTCTCATTGACTTGCAGCACCACAGGCTGTGAGTCCCCATTTTTCTCTTGGAGA
ACCCAGATAGATAGTCCACTGAATGGGAAGGTGACGAATGAGGGGACCACATCTACGCTG
ACAATGAATCCTGTTAGTTTTGGGAACGAACACTCTTACCTGTGCACAGCAACTTGTGAA
TCTAGGAAATTGGAAAAAGGAATCCAGGTGGAGATCTACTCTTTTCCTAAGGATCCAGAG
ATTCATTTGAGTGGCCCTCTGGAGGCTGGGAAGCCGATCACAGTCAAGTGTTCAGTTGCT
GATGTATACCCATTTGACAGGCTGGAGATAGACTTACTGAAAGGAGATCATCTCATGAAG
AGTCAGGAATTTCTGGAGGATGCAGACAGGAAGTCCCTGGAAACCAAGAGTTTGGAAGTA
ACCTTTACTCCTGTCATTGAGGATATTGGAAAAGTTCTTGTTTGCCGAGCTAAATTACAC
ATTGATGAAATGGATTCTGTGCCCACAGTAAGGCAGGCTGTAAAAGAATTGCAAGTCTAC
ATATCACCCAAGAATACAGTTATTTCTGTGAATCCATCCACAAAGCTGCAAGAAGGTGGC
TCTGTGACCATGACCTGTTCCAGCGAGGGTCTACCAGCTCCAGAGATTTTCTGGAGTAAG
AAATTAGATAATGGGAATCTACAGCACCTTTCTGGAAATGCAACTCTCACCTTAATTGCT
ATGAGGATGGAAGATTCTGGAATTTATGTGTGTGAAGGAGTTAATTTGATTGGGAAAAAC
AGAAAAGAGGTGGAATTAATTGTTCAAGAGAAACCATTTACTGTTGAGATCTCCCCTGGA
CCCCGGATTGCTGCTCAGATTGGAGACTCAGTCATGTTGACATGTAGTGTCATGGGCTGT
GAATCCCCATCTTTCTCCTGGAGAACCCAGATAGACAGCCCTCTGAGCGGGAAGGTGAGG
AGTGAGGGGACCAATTCCACGCTGACCCTGAGCCCTGTGAGTTTTGAGAACGAACACTCT
TATCTGTGCACAGTGACTTGTGGACATAAGAAACTGGAAAAGGGAATCCAGGTGGAGCTC
TACTCATTCCCTAGAGATCCAGAAATCGAGATGAGTGGTGGCCTCGTGAATGGGAGCTCT
GTCACTGTAAGCTGCAAGGTTCCTAGCGTGTACCCCCTTGACCGGCTGGAGATTGAATTA
CTTAAGGGGGAGACTATTCTGGAGAATATAGAGTTTTTGGAGGATACGGATATGAAATCT
CTAGAGAACAAAAGTTTGGAAATGACCTTCATCCCTACCATTGAAGATACTGGAAAAGCT
CTTGTTTGTCAGGCTAAGTTACATATTGATGACATGGAATTCGAACCCAAACAAAGGCAG
AGTACGCAAACACTTTATGTCAATGTTGCCCCCAGAGATACAACCGTCTTGGTCAGCCCT
TCCTCCATCCTGGAGGAAGGCAGTTCTGTGAATATGACATGCTTGAGCCAGGGCTTTCCT
GCTCCGAAAATCCTGTGGAGCAGGCAGCTCCCTAACGGGGAGCTACAGCCTCTTTCTGAG
AATGCAACTCTCACCTTAATTTCTACAAAAATGGAAGATTCTGGGGTTTATTTATGTGAA
GGAATTAACCAGGCTGGAAGAAGCAGAAAGGAAGTGGAATTAATTATCCAAGTTACTCCA
AAAGACATAAAACTTACAGCTTTTCCTTCTGAGAGTGTCAAAGAAGGAGACACTGTCATC
ATCTCTTGTACATGTGGAAATGTTCCAGAAACATGGATAATCCTGAAGAAAAAAGCGGAG
ACAGGAGACACAGTACTAAAATCTATAGATGGCGCCTATACCATCCGAAAGGCCCAGTTG
AAGGATGCGGGAGTATATGAATGTGAATCTAAAAACAAAGTTGGCTCACAATTAAGAAGT
TTAACACTTGATGTTCAAGGAAGAGAAAACAACAAAGACTATTTTTCTCCTGAGCTTCTC
GTGCTCTATTTTGCATCCTCCTTAATAATACCTGCCATTGGAATGATAATTTACTTTGCA
AGAAAAGCCAACATGAAGGGGTCATATAGTCTTGTAGAAGCACAGAAATCAAAAGTGTAG

Clone variation with respect to NM_001078.3
2208 g=>a
>OriGene 5' read for NM_001078 unedited
TCTTGGATTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGATCGGGCCTC
ACTGGCTTCAGGAGCTGAATACCCTCCCAGGCACACACAGGTGGGACACAAATAAGGGTT
TTGGAACCACTATTTTCTCATCACGACAGCAACTTAAAATGCCTGGGAAGATGGTCGTGA
TCCTTGGAGCCTCAAATATACTTTGGATAATGTTTGCAGCTTCTCAAGCTTTTAAAATCG
AGACCACCCCAGAATCTAGATATCTTGCTCAGATTGGTGACTCCGTCTCATTGACTTGCA
GCACCACAGGCTGTGAGTCCCCATTTTTCTCTTGGAGAACCCAGATAGATAGTCCACTGA
ATGGGAAGGTGACGAATGAGGGGACCACATCTACGCTGACAATGAATCCTGTTAGTTTTG
GGAACGAACACTCTTACCTGTGCACAGCAACTTGTGAATCTAGGAAATTGGAAAAAGGAA
TCCAGGTGGAGATCTACTCTTTTCCTAAGGATCCAGAGATTCATTTGAGTGGCCCTCTGG
AGGCTGGGAAGCCGATCACAGTCAAGTGTTCAGTTGCTGATGTATACCCATTTGACAGGC
TGGAGATAGACTTACTGAAAGGAGATCATCTCATGAAGAGTCAGGAATTTCTGGAGGATG
CAGACAGGAAGTCCCTGGAAACCAAGAGTTTGGAAGTAACCTTTACTCCTGTCATTGAGG
ATATTGGAAAAGTTCTTGTTTGCCGAGCTAAATTACACATTGATGAAATGGATTCTGTGC
CCACAGTAAGGCAGGCTGNTAAAGATTTGCAGTCTACATATCACCCAAGAATACAGTTAT
TTCTGTGAATCCATCCCCACAAGCTGCAGAAGGTGGCTCTGTGACCCATGACCTGTNCAG
CGAGGGTCTACCC
>OriGene 3' read for NM_001078 unedited
CGGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTAATTCCAGAATTATCACT
TTACTATACAAAAAAACCTAAACATTTACAAAAGAAAATAAAAAGAAGTTGCATTTTTAG
GCCACATTGGGAAAGTTGCACAGTAGTCTGATGAACAAACTTCGTGAAACATCTCCGTAC
CATGCCAGTACTATACAGAGAAACCCAGTCAACCATTATACCATAGGTTGAGGTATTTAG
TACCTGAAAGTCAACCCAGTGCTCCCTTTGCTTATTATTTGTCTATAAAAATATAAAACC
CGGCATCTTTACAAAACCTGAAATTATTGTCAATAAACTATATAGGAATAAAATTTTGTG
AAAGGGAATAAAATAGAAGAAAGATGACAAAGGTCAGCTGTCAGCAAATATTAATGTGAA
ATTTTCCTAACATGCATTAAATTATAATAGTCCAAGCAACACTAGTTATTTTAACAATCT
AAATATAGAGTGTTGCTATTTTAAGCAATCTTGCTATGGCATAATTTTATTTTACCTTTG
TCCAAATTAGGGTATTGGATTTACAAAACAAGGGAATTCCCCCGGTTTTTTTTTTGGGGG
GATCCGGTGTTTTGGGCGAAAAGTTTTTTTTTTTTTTTTCCCACAGGGGGGGAAAAGGGG
CTTTTTTTTCCCGGGAAATTTTTGGGGGGGGCGCCCCCCACCCATTTGTTTCTTGCGGGG
GGGGGGGGCCCCTCGGGGTGGCCCCACAAAAAGGTGGGCCCCGGGCACAAAAAAAAACGT
TGCCCCCCTTCTTGTGTGGGGGCGGGCACAAAAAAAAAAACACCCCCTCCGGGGGGGGGC
GCTTCCCCAACAAAACTAATTCTCCGCCGGGGGGGGGGG
Restriction Sites NotI-NotI     
ACCN NM_001078
Insert Size 3200 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001078.2, NP_001069.1
RefSeq Size 3119 bp
RefSeq ORF 2220 bp
Locus ID 7412
Cytogenetics 1p21.2
Domains ig, IGv, IGc2, IG
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
Gene Summary 'This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]'
Transcript Variant: This variant (1) encodes the predominant, full-length isoform (a).

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