VCAM1 (NM_001078) Human Untagged Clone

CAT#: SC119467

VCAM1 (untagged)-Human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VCAM1
• Synonyms: CD106; INCAM-100
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119467 sequence for NM_001078 edited (data generated by NextGen Sequencing)
  ATGCCTGGGAAGATGGTCGTGATCCTTGGAGCCTCAAATATACTTTGGATAATGTTTGCA
  GCTTCTCAAGCTTTTAAAATCGAGACCACCCCAGAATCTAGATATCTTGCTCAGATTGGT
  GACTCCGTCTCATTGACTTGCAGCACCACAGGCTGTGAGTCCCCATTTTTCTCTTGGAGA
  ACCCAGATAGATAGTCCACTGAATGGGAAGGTGACGAATGAGGGGACCACATCTACGCTG
  ACAATGAATCCTGTTAGTTTTGGGAACGAACACTCTTACCTGTGCACAGCAACTTGTGAA
  TCTAGGAAATTGGAAAAAGGAATCCAGGTGGAGATCTACTCTTTTCCTAAGGATCCAGAG
  ATTCATTTGAGTGGCCCTCTGGAGGCTGGGAAGCCGATCACAGTCAAGTGTTCAGTTGCT
  GATGTATACCCATTTGACAGGCTGGAGATAGACTTACTGAAAGGAGATCATCTCATGAAG
  AGTCAGGAATTTCTGGAGGATGCAGACAGGAAGTCCCTGGAAACCAAGAGTTTGGAAGTA
  ACCTTTACTCCTGTCATTGAGGATATTGGAAAAGTTCTTGTTTGCCGAGCTAAATTACAC
  ATTGATGAAATGGATTCTGTGCCCACAGTAAGGCAGGCTGTAAAAGAATTGCAAGTCTAC
  ATATCACCCAAGAATACAGTTATTTCTGTGAATCCATCCACAAAGCTGCAAGAAGGTGGC
  TCTGTGACCATGACCTGTTCCAGCGAGGGTCTACCAGCTCCAGAGATTTTCTGGAGTAAG
  AAATTAGATAATGGGAATCTACAGCACCTTTCTGGAAATGCAACTCTCACCTTAATTGCT
  ATGAGGATGGAAGATTCTGGAATTTATGTGTGTGAAGGAGTTAATTTGATTGGGAAAAAC
  AGAAAAGAGGTGGAATTAATTGTTCAAGAGAAACCATTTACTGTTGAGATCTCCCCTGGA
  CCCCGGATTGCTGCTCAGATTGGAGACTCAGTCATGTTGACATGTAGTGTCATGGGCTGT
  GAATCCCCATCTTTCTCCTGGAGAACCCAGATAGACAGCCCTCTGAGCGGGAAGGTGAGG
  AGTGAGGGGACCAATTCCACGCTGACCCTGAGCCCTGTGAGTTTTGAGAACGAACACTCT
  TATCTGTGCACAGTGACTTGTGGACATAAGAAACTGGAAAAGGGAATCCAGGTGGAGCTC
  TACTCATTCCCTAGAGATCCAGAAATCGAGATGAGTGGTGGCCTCGTGAATGGGAGCTCT
  GTCACTGTAAGCTGCAAGGTTCCTAGCGTGTACCCCCTTGACCGGCTGGAGATTGAATTA
  CTTAAGGGGGAGACTATTCTGGAGAATATAGAGTTTTTGGAGGATACGGATATGAAATCT
  CTAGAGAACAAAAGTTTGGAAATGACCTTCATCCCTACCATTGAAGATACTGGAAAAGCT
  CTTGTTTGTCAGGCTAAGTTACATATTGATGACATGGAATTCGAACCCAAACAAAGGCAG
  AGTACGCAAACACTTTATGTCAATGTTGCCCCCAGAGATACAACCGTCTTGGTCAGCCCT
  TCCTCCATCCTGGAGGAAGGCAGTTCTGTGAATATGACATGCTTGAGCCAGGGCTTTCCT
  GCTCCGAAAATCCTGTGGAGCAGGCAGCTCCCTAACGGGGAGCTACAGCCTCTTTCTGAG
  AATGCAACTCTCACCTTAATTTCTACAAAAATGGAAGATTCTGGGGTTTATTTATGTGAA
  GGAATTAACCAGGCTGGAAGAAGCAGAAAGGAAGTGGAATTAATTATCCAAGTTACTCCA
  AAAGACATAAAACTTACAGCTTTTCCTTCTGAGAGTGTCAAAGAAGGAGACACTGTCATC
  ATCTCTTGTACATGTGGAAATGTTCCAGAAACATGGATAATCCTGAAGAAAAAAGCGGAG
  ACAGGAGACACAGTACTAAAATCTATAGATGGCGCCTATACCATCCGAAAGGCCCAGTTG
  AAGGATGCGGGAGTATATGAATGTGAATCTAAAAACAAAGTTGGCTCACAATTAAGAAGT
  TTAACACTTGATGTTCAAGGAAGAGAAAACAACAAAGACTATTTTTCTCCTGAGCTTCTC
  GTGCTCTATTTTGCATCCTCCTTAATAATACCTGCCATTGGAATGATAATTTACTTTGCA
  AGAAAAGCCAACATGAAGGGGTCATATAGTCTTGTAGAAGCACAGAAATCAAAAGTGTAG
  
  Clone variation with respect to NM_001078.3
  2208 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001078 unedited
  TCTTGGATTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGATCGGGCCTC
  ACTGGCTTCAGGAGCTGAATACCCTCCCAGGCACACACAGGTGGGACACAAATAAGGGTT
  TTGGAACCACTATTTTCTCATCACGACAGCAACTTAAAATGCCTGGGAAGATGGTCGTGA
  TCCTTGGAGCCTCAAATATACTTTGGATAATGTTTGCAGCTTCTCAAGCTTTTAAAATCG
  AGACCACCCCAGAATCTAGATATCTTGCTCAGATTGGTGACTCCGTCTCATTGACTTGCA
  GCACCACAGGCTGTGAGTCCCCATTTTTCTCTTGGAGAACCCAGATAGATAGTCCACTGA
  ATGGGAAGGTGACGAATGAGGGGACCACATCTACGCTGACAATGAATCCTGTTAGTTTTG
  GGAACGAACACTCTTACCTGTGCACAGCAACTTGTGAATCTAGGAAATTGGAAAAAGGAA
  TCCAGGTGGAGATCTACTCTTTTCCTAAGGATCCAGAGATTCATTTGAGTGGCCCTCTGG
  AGGCTGGGAAGCCGATCACAGTCAAGTGTTCAGTTGCTGATGTATACCCATTTGACAGGC
  TGGAGATAGACTTACTGAAAGGAGATCATCTCATGAAGAGTCAGGAATTTCTGGAGGATG
  CAGACAGGAAGTCCCTGGAAACCAAGAGTTTGGAAGTAACCTTTACTCCTGTCATTGAGG
  ATATTGGAAAAGTTCTTGTTTGCCGAGCTAAATTACACATTGATGAAATGGATTCTGTGC
  CCACAGTAAGGCAGGCTGNTAAAGATTTGCAGTCTACATATCACCCAAGAATACAGTTAT
  TTCTGTGAATCCATCCCCACAAGCTGCAGAAGGTGGCTCTGTGACCCATGACCTGTNCAG
  CGAGGGTCTACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001078 unedited
  CGGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTAATTCCAGAATTATCACT
  TTACTATACAAAAAAACCTAAACATTTACAAAAGAAAATAAAAAGAAGTTGCATTTTTAG
  GCCACATTGGGAAAGTTGCACAGTAGTCTGATGAACAAACTTCGTGAAACATCTCCGTAC
  CATGCCAGTACTATACAGAGAAACCCAGTCAACCATTATACCATAGGTTGAGGTATTTAG
  TACCTGAAAGTCAACCCAGTGCTCCCTTTGCTTATTATTTGTCTATAAAAATATAAAACC
  CGGCATCTTTACAAAACCTGAAATTATTGTCAATAAACTATATAGGAATAAAATTTTGTG
  AAAGGGAATAAAATAGAAGAAAGATGACAAAGGTCAGCTGTCAGCAAATATTAATGTGAA
  ATTTTCCTAACATGCATTAAATTATAATAGTCCAAGCAACACTAGTTATTTTAACAATCT
  AAATATAGAGTGTTGCTATTTTAAGCAATCTTGCTATGGCATAATTTTATTTTACCTTTG
  TCCAAATTAGGGTATTGGATTTACAAAACAAGGGAATTCCCCCGGTTTTTTTTTTGGGGG
  GATCCGGTGTTTTGGGCGAAAAGTTTTTTTTTTTTTTTTCCCACAGGGGGGGAAAAGGGG
  CTTTTTTTTCCCGGGAAATTTTTGGGGGGGGCGCCCCCCACCCATTTGTTTCTTGCGGGG
  GGGGGGGGCCCCTCGGGGTGGCCCCACAAAAAGGTGGGCCCCGGGCACAAAAAAAAACGT
  TGCCCCCCTTCTTGTGTGGGGGCGGGCACAAAAAAAAAAACACCCCCTCCGGGGGGGGGC
  GCTTCCCCAACAAAACTAATTCTCCGCCGGGGGGGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001078
• Insert Size: 3200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001078.2, NP_001069.1
• RefSeq Size: 3119 bp
• RefSeq ORF: 2220 bp
• Locus ID: 7412
• Cytogenetics: 1p21.2
• Domains: ig, IGv, IGc2, IG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
• Gene Summary: 'This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (1) encodes the predominant, full-length isoform (a).