SRD5A1 (NM_001047) Human Untagged Clone

CAT#: SC119479

SRD5A1 (untagged)-Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRD5A1
• Synonyms: S5AR 1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001047, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAACGGCGACGGGGGTGGCGGAGGAGCGCCTGCTGGCCGCGCTCGCCTACCTGCAGTGCGCCGTGG
  GCTGCGCGGTCTTCGCGCGCAATCGTCAGACGAACTCAGTGTACGGCCGCCACGCGCTGCCCAGCCACAG
  GCTCCGAGTGCCGGCGCGGGCCGCCTGGGTGGTGCAGGAGCTGCCCTCGCTGGCCCTGCCGCTCTACCAG
  TACGCCAGCGAGTCCGCCCCGCGTCTCCGCAGCGCGCCCAACTGCATCCTCCTGGCCATGTTCCTCGTCC
  ACTACGGGCATCGGTGCTTAATTTACCCATTTCTGATGCGAGGAGGAAAGCCTATGCCACTGTTGGCGTG
  TACAATGGCGATTATGTTCTGTACCTGTAACGGCTATTTGCAAAGCAGATACTTGAGCCATTGTGCAGTG
  TATGCTGATGACTGGGTAACAGATCCCCGTTTTCTAATAGGTTTTGGCTTGTGGTTAACGGGCATGTTGA
  TAAACATCCATTCAGATCATATCCTAAGGAATCTCAGAAAACCAGGAGATACTGGATACAAAATACCAAG
  GGGAGGCTTATTTGAATACGTAACTGCAGCCAACTATTTTGGAGAAATCATGGAGTGGTGTGGCTATGCC
  CTGGCCAGCTGGTCTGTCCAAGGCGCGGCTTTTGCTTTCTTCACGTTTTGTTTTTTATCTGGTAGAGCAA
  AAGAGCATCATGAGTGGTACCTCCGGAAATTTGAAGAGTATCCAAAGTTCAGAAAAATTATAATTCCATT
  TTTGTTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001047 unedited
  GGGCACCATTTGTATACGACTCACTATAGGCGGCCCGCGATTCGGCACGAGGCTTGAGAA
  CCCTTTCTGCAGATTCCCGGCAGTGCGGGACTCCGGTAGCCGCCCCTCCGGTAGCCGCCC
  CTCCTGCCCCCGCGCCGACGACCTAATGTTGCCCGCCGCGGCCTCTGGGGCATGGAGCAC
  GCTGCCCAGCCCTGGCGATGGCAACGGCCACGGGGGCTGGCGGCAGCAGCGCCTGCTGGC
  CGCGCTCGCCTACCTGCAGTGCGCCGCGGGCTGCGCGGTCTTCGCGCGGAATCGTCATAC
  GAACTCAGAGTACGGACGACACGCGCTGTCCAGACACAGGCTCCGAGCGCCGGCGCGGTC
  CGCCTGGGTGGTGCAGGAGCTGCCCTCCCTGGCCCTGCCGCTCTACCAATACGCCAGCGA
  GATCGTCCCGAGTATCCGCAGCGCGCCCAACTGCATCCTACTGGCCATGTTCCTCGTCCA
  CTACAGGCATAGGCGCTTAATTTACACATTTCTGATGCGAGGAGGAAAGCCTATGCCACT
  GCTGGCGTGTACAACGGCGAATATGCTCTGTACCTGTAACGGCTATTTCCCAAGCAGATA
  CTTGAGCCATTGTGCAGCGCATGCTGACGACTGGGTAACAGATCCCCCGCTTCTAATAAG
  TCTTGGCTTGTGGTTAACGGGCATGTTGATACACATCCATTCAGATCATATCCTAAGGAA
  TCTCAGAAAACCAGGAGATACTGGATACACAATACCAAGGGGNAGGCTTTATTTGATACG
  TCACTGCAGCCCACTATTTTGGGAGAATCATGGAGTGGGTGTGGGTATGCCCCTGCCCAG
  CTGGTCTGCCCAAGGCGCGGGTTTTGCTTTCTTTACGAATGGTTTTTATCTGGTCAAGCA
  AAAGACATCATGAGTGGGACCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001047 unedited
  CGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAT
  GGCAAAGTCTATTTATTATGTTGTTAAAGACAACTGAAACAAATGGCATTTAAAAACTCC
  AGATGAAATGATAAGGCAAAGCAATGCCAGATGAATTCATCAAGATAAAAAGCAGTATGG
  CAATGTTTTTATCAAAGTAAAATTCAAGTCATGAAGCATTCAACAGGAAGATTATTTTAT
  ATTGATAACAGGTACAGGCTATGAGGGGAAAGCTTTGCAAAGCAACAACAGCAAAACAGA
  AAGCAAAAACTATTAAAAGGAAGATTACCTATGGAAAAAAAAAAAGGCTGTAAAAAATTT
  TAACAATTATTATGTTTCAATCAACAAAGAACAAACAGGATAAATCTTAAAAAGTGGTCT
  TGTTATTGTTACAAAAAGTGATCATTCTTTACACTACAAGGGAAAACTCAAAATCTACAA
  AAGAGAAATTTCACAGACAACAATCTCTACTATATCCATACGTGGGGAACAGAACAAAAA
  AACTAGGTCTAGACTAGAAGACAGAGAGAAACCATGTCAGACCAGAGCACATTGATGGCT
  CACGGTGAGTGACCCATGGCCTAAAGGTGCCTGTTTAGGGCCCAGGAGCCACTCACACAG
  GCAGAGCAGCTTACTCCGTATGAACCACCACCAGCACCCCTCCCTGCCCGGTGATGTCAC
  CCGGTGGGCTGGGCTGGAACAGTCCAGATGCCTTTGCCTCACCTGGAAAGGCCACGAGGA
  GCTTCCTCCTCTTCCCCAAGTTTCCACTTACCACAGCACCTGACACAATGTGGNCAAGTT
  TCCCCCGGGCATGACTGCACATGGCTACCCCAACCTTATGGCTCGAGAGAGCCTCTAAAC
  GTATTCACCCTTAGGGGNAGGAACAACCTGCATTGACCTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001047
• Insert Size: 2380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047.1, NP_001038.1
• RefSeq Size: 2285 bp
• RefSeq ORF: 780 bp
• Locus ID: 6715
• Cytogenetics: 5p15.31
• Domains: Steroid_dh
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism
• Gene Summary: 'Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]'