RPL18A (NM_000980) Human Untagged Clone

CAT#: SC119487

RPL18A (untagged)-Human ribosomal protein L18a (RPL18A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL18A
• Synonyms: L18A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119487 sequence for NM_000980 edited (data generated by NextGen Sequencing)
  ATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCC
  AAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCC
  AAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAG
  ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC
  TGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTG
  ACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACATGGGTGCCCGGCACCGCGCCCGA
  GCCCACTCCATTCAGATCATGAAGGTGGAGGAGATCGCGGCCAGCAAGTGCCGCCGGCCG
  GCTGTCAAGCAGTTCCACGACTCCAAGATCAAGTTCCCGCTGCCCCACCGGGTCCTGCGC
  CGTCAGCACAAGCCACGCTTCACCACCAAGAGGCCCAACACCTTCTTCTAG
  
  Clone variation with respect to NM_000980.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000980 unedited
  GCACGAGGCCTCGTGCCGAATTCGGCACGAGGGCGGAGAGCACGCCATGAAGGCCTCGGG
  CACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCC
  GCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTG
  GTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGG
  GCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGA
  CTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGC
  TGTCACCCAGTGCTACCGAGACATGGGTGCCCGGCACCGCGCCCGAGCCCACTCCATTCA
  GATCATGAAGGTGGAGGAGATCGCGGCCAGCAAGTGCCGCCGGCCGGCTGTCAAGCAGTT
  CCACGACTCCAAGATCAAGTTCCCGCTGCCCCACCGGGTCCTGCGCCGTCAGCACAAGCC
  ACGCTTCACCACCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000980 unedited
  GGGGGGGCCCCCGGCCCCCCCCCCCCCGGCCCCCCCCCCCCGNCTCTTTGCCGTTCCCTG
  CGATTTCTATTGGGGTCACACCCGGNCGNAGGGCCCTGCACCTANAANAAGTGTTGGGCC
  TCTTGGTGGTGAAGCGTGGCTTGTGCTGACGGCGCAGGACCCGGTGGGGCAGCGGGAACT
  TGATCTTGGAGTCGTGGAACTGCTTGACAGCCGGCCGGCGGCACTTGCTGGCCGCGATCT
  CCTCCACCTTCATGATCTGAATGGAGTGGGCTCGGGCGCGGTGCCGGGCACCCATGTCTC
  GGTAGCACTGGGTGACAGCGCCTGCGGCGGTCAGGTCCCGGTATTCCCGGTACATGTTGT
  GGGTGCCGCTCCGGGAGTCATAGCGCAGCCAGATCCCGAAGTTCTTCACCCGCAGGGGGG
  ACTTCTCAAACACCTGCCCACAGTAGACAATTTCCCCTGAAGACTTCTTCATCTTCTTTA
  ACTGACATACAAAGTACCACAACCGGGACTCGGCGACCACATGATTACGCCCAAAGATTC
  TCATGCGGTATAGGGGCGGCCCGTGGCATTTTGGGGGTGGGCAGGCAGCGACCCACTACC
  TTGTTCCTCTCGTCTCCGTGCCCGATGCCTTTATGGCGTGCTTTCTCGTCCTCGTGCCCG
  AATTCCGCACCGACGCCCTATGCCGAATTCCCCGGGCCGCCCTTTTCTGATTTTATTACA
  TAATTCTGACGGTTTACTTAACAATTTTTGTTTATTTTTACCTTCCATCGTTCACCCCTA
  CCCGCCCTTTGCCTCATCGGGGGGGGGTCCTTACCTCTTTTTGGGAAATTCCCTTTGTTT
  TTTCGTGCTCTAACCAAACTTCCCTTGACCTCCACGCCTGCCGCGCACCTGGCCAATACT
  CCCGAATCACATCCTTTCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000980
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000980.2, NP_000971.1
• RefSeq Size: 618 bp
• RefSeq ORF: 531 bp
• Locus ID: 6142
• Cytogenetics: 19p13.11
• Domains: Ribosomal_L18ae
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). This gene is co-transcribed with the U68 snoRNA, located within the third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. [provided by RefSeq, Jul 2012]'