RPL21 (NM_000982) Human Untagged Clone

CAT#: SC119489

RPL21 (untagged)-Human ribosomal protein L21 (RPL21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL21
• Synonyms: HYPT12; L21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119489 sequence for NM_000982 edited (data generated by NextGen Sequencing)
  ATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGTTCTCTAGGCCTTTTAGA
  AAACATGGAGTTGTTCCTTTGGCCACATATATGCGAATCTATAAGAAAGGTGATATTGTA
  GACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAA
  ACTGGAAGAGTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTT
  AAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCACTCTAAG
  AGCCGAGATAGCTTCCTGAAACGTGTGAAGGAAAATGATCAGAAAAAGAAAGAAGCCAAA
  GAGAAAGGTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAGAGAAGCACACTTT
  GTGAGAACCAATGGGAAGGAGCCTGAGCTGCTGGAACCTATTCCCTATGAATTCATGGCA
  TAA
  
  Clone variation with respect to NM_000982.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000982 unedited
  CAAATTTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCGCCATCTTCCA
  GTAATTCGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGTTCTC
  TAGGCCTTTTAGAAAACATGGAGTTGTTCCTTTGGCCACATATATGCGAATCTATAAGAA
  AGGTGATATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTG
  TTACCATGGCAAAACTGGAAGAGTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGT
  AAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACAT
  TAAGCACTCTAAGAGCCGAGATAGCTTCCTGAAACGTGTGAAGGAAAATGATCAGAAAAA
  GAAAGAAGCCAAAGAGAAAGGTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAG
  AGAAGCACACTTTGTGAGAACCAATGGGAAGGAGCCTGAGCTGCTGGAACCTATTCCCTA
  TGAATTCATGGCATAATAGGTGTTAAAAAAAAAAATAAAGGACCTCTGGGCTACAAAAAA
  AAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATC
  CCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTC
  CAGTGCCCACCAGCCTTGTCCTAATAAAATTAANGTGCATCATTTTGTCTGACTAGGTGT
  CCTTCATAAAATTATGGGGGTGGAGGGGGTTGGGGTTTTGAAACCAGGTGGCCAGTTTGG
  GAACAACACCTGTACGGCCTGCGNGGGTCTATTGGGAACCAACTGTATTGCAGTGCCACA
  ATCTGGCTCACTGCATCTNCGCCTCCTGGGTTCAGCGATNTCTCTGCTAGCTCCCGAGNT
  GNTGGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000982 unedited
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAGCCCAGAGGTCCTTTATTTTTTTTTTTA
  ACACCTATTATGCCATGAATTCATAGGGAATAGGTTCCAGCAGCTCAGGCTCCTTCCCAT
  TGGTTCTCACAAAGTGTGCTTCTCTGGGTGGAGCAGGCTGGCGCTTTAGTTGAACCCAGG
  TACCTTTCTCTTTGGCTTCTTTCTTTTTCTGATCATTTTCCTTCACACGTTTCAGGAAGC
  TATCTCGGCTCTTAGAGTGCTTAATGTGCTCAATACGCACATTAATTCTCTTGGCAAGAA
  TCTTGCCCTTAACTTGTTTGTTTACAACAATGCCAACAGCATGCTGGGTAACATTGTAGA
  CTCTTCCAGTTTTGCCATGGTAACACTTGTGGGGCATTCCTTTTTGAACAGTACCCATTC
  CCTTGATGTCTACAATATCACCTTTCTTATAGATTCGCATATATGTGGCCAAAGGAACAA
  CTCCATGTTTTCTAAAAGGCCTAGAGAACATATATCGGGTGCCTCTCCTCTTTCCCTTTG
  TGTTCGTCATTTTGGCGAATTACTGGAAGATGGCGCCTCGTGCCGAATTCGCGGCCGCCC
  TATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGAC
  CTNCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGGCGGGGGTTATTACGACATT
  TTTGGAAAGTCCCGTTGATTTTGGGTGCAAACAAACTCCATTGACGTCATGGGGTGGAGA
  CTTGNAATTCCCGTGAGTCAAACGTTTCCCGCCCATTGTGTACTGCCAAACCGATCACAT
  GGTAATACGAGACTATACCTAAATGCCTGCAATAGGAAATCCCCTAGGTCTGACTTGGCT
  ATGCCAGCGGGCATTACCGCATGACTCAAAGGGGCGGCTGCCTAGAACCTGATGACTGCA
  AGGGCGTTACCGAAACTCCCCCTGCGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000982
• Insert Size: 620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000982.2, NP_000973.2
• RefSeq Size: 568 bp
• RefSeq ORF: 483 bp
• Locus ID: 6144
• Cytogenetics: 13q12.2
• Domains: Ribosomal_L21e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'