RPL27 (NM_000988) Human Untagged Clone

CAT#: SC119494

RPL27 (untagged)-Human ribosomal protein L27 (RPL27)


  "NM_000988" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RPL27
Synonyms DBA16; L27
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119494 sequence for NM_000988 edited (data generated by NextGen Sequencing)
ATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGACGCTACTCCGGA
CGCAAAGCTGTCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCCCCTACAGCCAT
GCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCATGGGCAAGAAG
AAGATCGCCAAGAGATCAAAGATAAAATCTTTTGTGAAAGTGTATAACTACAATCACCTA
ATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAACTGTCGTCAATAAGGATGTC
TTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCAAGTTTGAAGAG
AGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTTAG

Clone variation with respect to NM_000988.3
>OriGene 5' read for NM_000988 unedited
GGTGCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGTGG
TTGCTGCCGAAATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGAC
GCTACTCCGGACGCAAAGCTGTCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCC
CCTACAGCCATGCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCA
TGGGCAAGAAGAAGATCGCCAAGAGATCAAAGATAAAATCTTTTGTGAAAGTGTATAACT
ACAATCACCTAATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAACTGTCGTCA
ATAAGGATGTCTTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCA
AGTTTGAAGAGAGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTT
AGATGCTTTGTTTTGATCATTAAAAATTATAAAGAAAAAAAAAAAAAAAAAACTCGACTC
TAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGAC
CCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTC
CTAATAAAATTAAGTTGCATCATTNTGTCTGACTAGGTGTCCTTCTATAATATTATGGGG
TGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTTAGGCCTGCGGG
GTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATTCTGGCTCACTGCAATCTCCCCC
TCCTGGGTCCAAGCGATNCTTCTGCCTCAACCTNCCCGANTTGTTGGGATTCCAGGCTGG
CTGACCAGGCCCAAGTAATTT
>OriGene 3' read for NM_000988 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTATAATTTTTAATGATCAAA
ACAAAGCATCTAAAACCGCAGTTTCTGGAAGAACCACTTGTTCTTGCCTGTCTTGTATCT
CTCTTCAAACTTGACCTTGGCCTCCCGTCGGGCCTTGCGTTTAAGAGCAGGATCTCTGAA
GACATCCTTATTGACGACAGTTTTGTCCAAGGGGATATCCACAGAGTACCTTGTGGGCAT
TAGGTGATTGTAGTTATACACTTTCACAAAAGATTTTATCTTTGATCTCTTGGCGATCTT
CTTCTTGCCCATGGCAGCTGTCACTTTGCGGGGGTAGCGGTCAATTCCAGCCACCAGAGC
ATGGCTGTAGGGGCGATCTGAGGTGCCATCATCAATGTTCTTCACGATGACAGCTTTGCG
TCCGGAGTAGCGTCCAGCCAGGACAAGCACCACCTTCCCAGGTTTCATGAACTTGCCCAT
TTCGGCAGCAACCACCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACA
CAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAAACCTCCCACCGTACACGCCTA
CCGTCCATTTGCGTAACGGGGCCGGGTTATTACCACCTTTTGGAAAGTCCCGTTGTTTTG
GTGCCAAACCAAACCTCCATTGGAGGTAATGGGGTGGAGACTCGGGAATCCCCGGAGTCA
ACCGTTCCCCAGCCCCTGGGTGACTGGCACACCGGCCCCCATGGTATAGCATCACTTATA
CGAAACGCCCCCCATTCGGCCAGCCCCCCAGGCGCTGATGGGTTTTATGTTTTTGGCGAT
TCTACGGACACGCCGCCCAAGGGGCGCCGCGCGTACCACCCCTCATCCCCTCCACTCTTA
TTCCCCGCACTTTCCCCCTACCGTCGTGCAGCCCTCTTGGTTGTTCGCGCCTTCCC
Restriction Sites NotI-NotI     
ACCN NM_000988
Insert Size 510 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000988.2, NP_000979.1
RefSeq Size 513 bp
RefSeq ORF 411 bp
Locus ID 6155
Cytogenetics 17q21.31
Domains Ribosomal_L27e, KOW, KOW
Protein Families Druggable Genome
Protein Pathways Ribosome
Gene Summary 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]'
Transcript Variant: This variant (1) encodes the protein. Variants 1-3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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