RPL27A (NM_000990) Human Untagged Clone

CAT#: SC119496

RPL27A (untagged)-Human ribosomal protein L27a (RPL27A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL27A
• Synonyms: L27A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119496 sequence for NM_000990 edited (data generated by NextGen Sequencing)
  ATGCCATCCAGACTGAGGAAGACCCGGAAACTTAGGGGCCACGTGAGCCACGGCCACGGC
  CGCATAGGCAAGCACCGGAAGCACCCCGGCGGCCGCGGTAATGCTGGTGGTCTGCATCAC
  CACCGGATCAACTTCGACAAATACCACCCAGGCTACTTTGGGAAAGTTGGTATGAAGCAT
  TACCACTTAAAGAGGAACCAGAGCTTCTGCCCAACTGTCAACCTTGACAAATTGTGGACT
  TTGGTCAGTGAACAGACACGGGTGAATGCTGCTAAAAACAAGACTGGGGCTGCTCCCATC
  ATTGATGTGGTGCGATCGGGCTACTAYAAAGTTCTGGGAAAGGGAAAGCTCCCAAAGCAG
  CCTGTCATCGTGAAGGCCAAATTCTTCAGCAGAAGAGCTGAGGAGAAGATTAAGAGTGTT
  GGGGGGGCCTGTGTCCTGGTGGCTTGA
  
  Clone variation with respect to NM_000990.4
  327 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000990 unedited
  GGTAACCGACTTGGATTTTGTAATCCGACTTCACTATAGGNCGGCACGCGCAATTCGGCA
  CGAGGCCTCGTGCCGAATTCGGCACGAGGGCCAACATGCCATCCAGACTGAGGAAGACCC
  GGAAACTTAGGGGCCACGTGAGCCACGGCCACGGCCGCATAGGCAAGCACCGGAAGCACC
  CCGGCGGCCGCGGTAATGCTGGTGGTCTGCATCACCACCGGATCAACTTCGACAAATACC
  ACCCAGGCTACTTTGGGAAAGTTGGTATGAAGCATTACCACTTAAAGAGGAACCAGAGCT
  TCTGCCCAACTGTCAACCTTGACAAATTGTGGACTTTGGTCAGTGAACAGACACGGGTGA
  ATGCTGCTAAAAACAAGACTGGGGCTGCTCCCATCATTGATGTGGTGCGATCGGGCTACT
  ATAAAGTTCTGGGAAAGGGAAAGCTCCCAAAGCAGCCTGTCATCGTGAAGGCCAAATTCT
  TCAGCAGAAGAGCTGAGGAGAAGATTAAGAGTGTTGGGGGGGCCTGTGTCCTGGTGGCTT
  GAAGCCACATGGAGGGAGTTTCATTAAATGCTAACTACTTTTAAAAAAAAAAAAANNAAN
  NAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGCCTCTAGATTGCGGCCGCGGTCATAG
  CTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGG
  CCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCA
  TTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAGGGGTGGGGAATTGAAACC
  AGGGGCAAATTGGGGAAAACAACCTGTNGGGCCCTGCGGGGTCTATTGGGAAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000990 unedited
  NTAGCTTTGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTAAAAGGAGTTAGCATTTAATGAAACTCCCTCCATGG
  GGCTTAAAGCCACCAGGACACAGGCCCCCCCAACACTTTTAATCTTCTCCTCAGCTTTTT
  TGCTGAAAAATTTGGCCTTCACAATGACAGGCTGCTTTGGGAGCTTTCCCTTTCCCAAAA
  CTTTATAGTACCCCGATCGCCCCACATCAATGATGGGAGCAGCCCCAGTCTTGTTTTTAG
  CAGCATTCACCCGTGTTTGTTCACTGACCAAAGTCCACAATTTGTCAAGGTTGACAGTGG
  GGCAAAAGCTCTGGTTCCTTTTTAAGGGGAAATGCTTCATACCAACTTTCCCAAAGTAGC
  CTGGGGGGTATTTGCCAAAGTTGATCCGGGGGTGATGCAAACCACCATCATTACCGGGGC
  CGCCGGGGTGCTTCCGGTGCTTGCCTATGCGGCCGTGGCCGGGGCTTACGGGGCCCCTAA
  GTTTCCGGGTCTTCCTCAGTCTGGATGGAATGTTGGCCCTTGCGCCGAATTCGGCACGAG
  GCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATACCAAAATTCCGACGGTCC
  ACTAAACGAGCTGTGTTTATATAAATCTTCCACCGTACCCGCCTACCGGCCATTTGCGCA
  AAAGGGGGCGGGGCTTATTACCACATNTTGGAAAGCCCCGTGGATTTTGGCGCCAAAACA
  AACTCCCATTGAGGTAAATGGGGTGGAAACTTGGAACTCCCCGGGAGCCAACCGCTTTCC
  CCCCCCTTGGGGGACTGCCAAAACCCCTCCCCTGGTAATAGCCATGACAACACCGAAATG
  TCCTGGCAAGTAAGAACTCTCTTTAGGCATTGTCCGGTCATTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000990
• Insert Size: 430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000990.2, NP_000981.1
• RefSeq Size: 514 bp
• RefSeq ORF: 447 bp
• Locus ID: 6157
• Cytogenetics: 11p15.4
• Domains: L15
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]'