RPL31 (NM_000993) Human Untagged Clone

CAT#: SC119500

RPL31 (untagged)-Human ribosomal protein L31 (RPL31), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL31
• Synonyms: L31
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119500 sequence for NM_000993 edited (data generated by NextGen Sequencing)
  ATGGCTCCCGCAAAGAAGGGTGGCGAGAAGAAAAAGGGCCGTTCTGCCATCAACGAAGTG
  GTAACCCGAGAATACACCATCAACATTCACAAGCGCATCCATGGAGTGGGCTTCAAGAAG
  CGTGCACCTCGGGCACTCAAAGAGATTCGGAAATTTGCCATGAAGGAGATGGGAACTCCA
  GATGTGCGCATTGACACCAGGCTCAACAAAGCTGTCTGGGCCAAAGGAATAAGGAATGTG
  CCATACCGAATCCGTGTGCGGCTGTCCAGAAAACGTAATGAGGATGAAGATTCACCAAAT
  AAGCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTACAGACAGTC
  AATGTGGATGAGAACTAA
  
  Clone variation with respect to NM_000993.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000993 unedited
  TTCGGCACGAGGCTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGCGAGAAGAAA
  AGGGCCGTTCTGCCATCAACGAAGTGGTAACCCGAGAATACACCATCAACATTCACAAGC
  GCATCCATGGAGTGGGCTTCAAGAAGCGTGCACCTCGGGCACTCAAAGAGATTCGGAAAT
  TTGCCATGAAGGAGATGGGAACTCCAGATGTGCGCATTGACACCAGGCTCAACAAAGCTG
  TCTGGGCCAAAGGAATAAGGAATGTGCCATACCGAATCCGTGTGCGGCTGTCCAGAAAAC
  GTAATGAGGATGAAGATTCACCAAATAAGCTATATACTTTGGTTACCTATGTACCTGTTA
  CCACTTTCAAAAATCTACAGACAGTCAATGTGGATGAGAACTAATCGCTGATCGTCAGAT
  CAAATAAAGTTATAAAATTGCAAAAAAAAAAAAAAAAAACTCGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000993 unedited
  CGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGCAATTTTATAACTTTATTT
  GATCTGACGATCAGCGATTAGTTCTCATCCACATTGACTGTCTGTAGATTTTTGAAAGTG
  GTAACAGGTACATAGGTAACCAAAGTATATAGCTTATTTGGTGAATCTTCATCCTCATTA
  CGTTTTCTGGACAGCCGCACACGGATTCGGTATGGCACATTCCTTATTCCTTTGGCCCAA
  ACAGCTTTGTTGAGCCTGGTGTCAATGCGCACATCTGGAGTTCCCATCTCCTTCATGGCA
  AATTTCCGAATCTCTTTGAGTGCCCGAGGTGCACGCTTCTTGAAGCCCACTCCATGGATG
  CGCTTGTGAATGTTGATGGTGTATTCTCGGGTTACCACTTCGTTGATGGCAGAACGGCCC
  TTTTTCTTCTCGCCACCCTTCTTTGCGGGAGCCATTCTGCAGCGTCCAAGCCTCGTGCCG
  AATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGC
  TCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGG
  GTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGAC
  GTCAATGGGGTNGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGG
  TGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCC
  AAGTAAGAAAGTCCCGTAAAGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTCCGTC
  ATTGACGTCATAGGGGGCGGACTTGCATATGATCACTNGATGTACTGCCAGTGGGCAGTT
  TACGTAAATACTCACCATTGACGTCATGGAAGTCCCTTATGCGTACTAGGGAACATCGTC
  ATATTGACTCAAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000993
• Insert Size: 480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000993.2, NP_000984.1
• RefSeq Size: 442 bp
• RefSeq ORF: 378 bp
• Locus ID: 6160
• Cytogenetics: 2q11.2
• Domains: Ribosomal_L31e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1.