RPLP1 (NM_001003) Human Untagged Clone

CAT#: SC119510

RPLP1 (untagged)-Human ribosomal protein, large, P1 (RPLP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPLP1
• Synonyms: LP1; P1; RPP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119510 sequence for NM_001003 edited (data generated by NextGen Sequencing)
  ATGGCCTCTGTCTCCGAGCTCGCCTGCATCTACTCGGCCCTCATTCTGCACGACGATGAG
  GTGACAGTCACGGAGGATAAGATCAATGCCCTCATTAAAGCAGCCGGTGTAAATGTTGAG
  CCTTTTTGGCCTGGCTTGTTTGCAAAGGCCCTGGCCAACGTCAACATTGGGAGCCTCATC
  TGCAATGTAGGGGCCGGTGGACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAGGTCCT
  GCCCCCTCCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAA
  TCCGAGGAGTCTGATGATGACATGGGCTTTGGTCTTTTTGACTAA
  
  Clone variation with respect to NM_001003.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001003 unedited
  TTTTGTATACGACTCCTATAGGCGGCCGCGACATTCGCACGAGGCTGCCGCCAGGTGCTC
  GGTCCTTCCGAGGAAGCTAAGGCTGCGTTGGGGTGAGGCCCTCACTTCATCCGGCGACTA
  GCACCGCGTCCGGCAGCGCCAGCCCTACACTCGCCCGCGCCATGGCCTCTGTCTCCGAGC
  TCGCCTGCATCTACTCGGCCCTCATTCTGCACGACGATGAGGTGACAGTCACGGAGGATA
  AGATCAATGCCCTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGT
  TTGCAAAGGCCCTGGCCAACGTCAACATTGGGAGCCTCATCTGCAATGTAGGGGCCGGTG
  GACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAGGTCCTGCCCCCTCCACTGCTGCTG
  CTCCAGCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATG
  ACATGGGCTTTGGTCTTTTTGACTAAACCTCTTTTATAACATGTTCAATAAAAAGCTGAA
  CTTTAAAAAANNAAAANNNAAAAAAAAAAAAAAAAAAAANANCCTCGACTCTAGATTGCG
  GCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCA
  GTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAA
  TTAGTTGCATCATTTTGTCTGACTAGGTGGCCTCTATAATATGGGGNGGGGGNGNGGGNN
  NNNNNNNNNNNCGGGGGGGGNNNNNNGGGGGGAAAACCCNCTGGGGGCGGGGGGGGGGGT
  TTTATGGGAAAACCATTGGGGGGGGGGGGGGGGACAATTTGGGGTTACTGGATCTCCCCT
  TCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001003 unedited
  CCTTTAGCTTGNCCGCGGCCGCTATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTAAAAGGTCAGCTTTTTATTGAACATGTTATAAAAGAGGTTTAGTCAA
  AAAGACCAAAGCCCATGTCATCATCAAACTCCTCGGATTCTTCTTTCTTTGCTTCCACTT
  TCTTCTCCTCAGCTGGAGCAGCAGCAGGGGAGGGGGCAGGACCTCCTGCTGGGGCAGCAC
  CAGCTGCTGGAGCAGGTCCACCGGCCCCTACATTGCAAATGAGGCTCCCAATGTTGACGT
  TGGCCAGGGCCTTTGCAAACAAGCCAGGCCAAAAAGGCTCAACATTTACACCGGCTGCTT
  TAATGAGGGCATTGATCTTATCCTCCGGGACTGTCACCTCATCGTCGGGCAAAATGAGGG
  CCGAGTAAATGCAGGCGAGCTCGGAGACAGAGGCCATGGCGCGGGCGAGTGTAGGGCTGG
  CGCTGCCGGACGCGGTGCTAGTCGCCGGATGAAGTGAGGGCCTCACCCCAACGCAGCCTT
  AGCTTCCTCGGAAGGACCGAGCACCTTGGCGGAAGCCTCGTGCCGAATTCGCGGCCGCCC
  TATAGGGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATAAGAAC
  CTCCACCGGACACGCCTACCGCCCATTGGGGTAAACGGGGGGGGGTTATTCCGACATTTT
  GGAAAGCCCCGTGGATTTTGGTGGCCAAACAAACTCCCCTTGGCGTCAATGGGGGGGGAA
  ACTTGAAATCCCCGGGGGGCAAACCGCTATCCACGCCCATTGGGGTACTGCCAAACCGCA
  ACCCATGGGAAAAGGGAGGACAAAACCCAAAAGCCCTGCCAGTNGGAAAACCCCCCAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001003
• Insert Size: 560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003.2, NP_000994.1
• RefSeq Size: 512 bp
• RefSeq ORF: 345 bp
• Locus ID: 6176
• Cytogenetics: 15q23
• Domains: 60s_ribosomal
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This predominant variant (1) is the longer transcript and encodes the longer isoform (1).