RPS15A (NM_001019) Human Untagged Clone

CAT#: SC119526

RPS15A (untagged)-Human ribosomal protein S15a (RPS15A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS15A
• Synonyms: DBA20; S15a
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001019 edited
  ATGGTGCGCATGAATGTCCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGA
  GGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAGTCATCGTCCGGTTTCTCACTGTG
  ATGATGAAGCATGGTTACATTGGCGAATTTGAAATCATTGATGACCACAGAGCTGGGAAA
  ATTGTTGTGAACCTCACAGGCAGGCTAAACAAGTGTGGGGTGATCAGCCCCAGATTTGAC
  GTGCAACTCAAAGACCTGGAAAAATGGCAGAATAATCTGCTTCCATCCCGCCAGTTTGGT
  TTCATTGTACTGACAACCTCAGCTGGCATCATGGACCATGAAGAAGCAAGACGAAAACAC
  ACAGGAGGGAAAATCCTGGGATTCTTTTTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001019 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTTCCGCGCCGCCACAAT
  GGTGCGCATGAATGTCCTGGCAGATGCTCTCAAGAGTATCAACAATGGCCGAAAAGAGAG
  GCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAGTCATCGTCCGGTTTCTCACTGTGA
  TGATGAAGCATGGTTACATTGGCGAATTTGAAATCATTGATGACCACAGAGCTGGGAAAA
  TTGTTGTGAACCTCACAGGCAGGCTAAACAAGTGTGGGGTGATCAGCCCCAGATTTGACG
  TGCAACTCAAAGACCTGGAAAAATGGCAGAATAATCTGCTTCCATCCCGCCAGTTTGGTT
  TCATTGTACTGACAACCTCAGCTGGCATCATGGACCATGAAGAAGCAAGACGAAAACACA
  CAGGAGGGAAAATCCTGGGATTCTTTTTCTAGGGATGTAATACATATATTTACAAATAAA
  ATGCCTCATGGACTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGCTTTTAAATGGGGGCCG
  GGGTCTTAGTTGTTTCTTGAAAAAATCCCGGGGGGATTCCTTGTAACCCCTCCCCAGGGC
  CTTTCCTGGCCTTGAAAGTTGCCACTTCAGGGCCAACCAGCTTTGGCCCAAAAAAATAAA
  GTGCATATTTTTGGCTGAACAAGGGGCCCTTCTATAATTTATGGGGGGAGGGGGGGGTGG
  TTTGGGACAAGGGCCCATTTTGAGAAAAACACTGGTAGGCTGGGGGGGGTCATTGGAAAC
  AATCTGGAGTGCAGGGCCCATTTTGGTTATTGCANTTTCGCTTCTGGTTTAAACGATTTC
  CTGCCTACCCCCCGAGTTGGGGGTTCCCGCTTGCTGACCGCTACTTATTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001019 unedited
  AGGAAACTGGGGGAGNCTAGTCTTCCGTCAATGTAGTATTACATACCCTANAAAAANATC
  CCAGATTTTTTCTCCTGTGTGTTTTCGTCTTGCTTCTTCATGGTCCATGATGCCAGCTGA
  GGTTGTCAGTACAATGAAACCAAACTGGCGGGATGGAAGCAGATTATTCTGCCATTTTTC
  CAGGTCTTTGAGTTGCACGTCAAATCTGGGGCTGATCACTCCTACGTGTTTAGCCTGCCT
  GTGAGGTTCACAACAATTTTCCCAGCTCTGTGGTCATCAATGATTTCAAATTCGCCAATG
  TAACCATGCTTCATCATCACAGTGAGAAACCGGACGATGACTTTGGAGCACGGCCTAATA
  AGCACCTGGCGTTTGCCTCTCTTTTCGGCATTGTTGATACTCTTGAGAGCATCTGCCAGG
  ACATTCATGCGCACCATTGTGGCGGCGCGGAAACCTCGTGCCGAATTCGCGGCCGCCCTA
  TAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCT
  CCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGGCGGGGTTATCACGACATTTTG
  GAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGCGGGAGA
  CTTGGTAATCCCCCGCGAGTCAAACCGCCTTCCACGCCCATTGGTGGTCTGCCTAAACCG
  CATCACCTTGGTTATAGTGCCGGCTTATGCGTTACATGTCCTGGCCTAGTTGGCAAGTCC
  CGTANGGGCATGGTCCGGCGCTTATTGCCCGCCGGGCGCTTTTCCGTTATTGACGCCCTT
  AAGCGGGCGGCCTCGGCCTCTGTACACCTCTGTTGCACTGCGCAGGGGCCGGTTCTCCGT
  AATTCCCCACCATCCGCGATAATGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001019
• Insert Size: 560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001019.4, NP_001010.2
• RefSeq Size: 554 bp
• RefSeq ORF: 393 bp
• Locus ID: 6210
• Cytogenetics: 16p12.3
• Domains: Ribosomal_S8
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) is the longest transcript and utilizes an alternate splice site on the 5'-terminal exon, compared to variant 1. Variants 1 and 2 encode the same protein.