RPS19 (NM_001022) Human Untagged Clone

CAT#: SC119529

RPS19 (untagged)-Human ribosomal protein S19 (RPS19)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS19
• Synonyms: DBA; DBA1; eS19; LOH19CR1; S19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119529 sequence for NM_001022 edited (data generated by NextGen Sequencing)
  ATGCCTGGAGTTACTGTAAAAGACGTGAACCAGCAGGAGTTCGTCAGAGCTCTGGCAGCC
  TTCCTCAAAAAGTCCGGGAAGCTGAAAGTCCCCGAATGGGTGGATACCGTCAAGCTGGCC
  AAGCACAAAGAGCTTGCTCCCTACGATGAGAACTGGTTCTACACGCGAGCTGCTTCCACA
  GCGCGGCACCTGTACCTCCGGGGTGGCGCTGGGGTTGGCTCCATGACCAAGATCTATGGG
  GGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCGAGGCTCCAAGAGTGTGGCC
  CGCCGGGTCCTCCAAGCCCTGGAGGGGCTGAAAATGGTGGAAAAGGACCAAGATGGCGGC
  CGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGCAGCT
  GCCAACAAGAAGCATTAG
  
  Clone variation with respect to NM_001022.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001022 unedited
  NGGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGC
  CGAATTCGGCACGAGGGGTGGCGCCCGGGNAAACCCCGTCGTTCCCTTTCCCCTGGCTGG
  CAGCGCGGAGGCCGCACGATGCCTGGAGTTACTGTAAAAGACGTGAACCAGCAGGAGTTC
  GTCAGAGCTCTGGCAGCCTTCCTCAAAAAGTCCGGGAAGCTGAAAGTCCCCGAATGGGTG
  GATACCGTCAAGCTGGCCAAGCACAAAGAGCTTGCTCCCTACGATGAGAACTGGTTCTAC
  ACGCGAGCTGCTTCCACAGCGCGGCACCTGTACCTCCGGGGTGGCGCTGGGGTTGGCTCC
  ATGACCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCGA
  GGCTCCAAGAGTGTGGCCCGCCGGGTCCTCCAAGCCCTGGAGGGGCTGAAAATGGTGGAA
  AAGGACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAATC
  GCCGGACAGGTGGCAGCTGCCAACAAGAAGCATTAGAACAAACCATGCTGGGTTAATAAA
  TTGCCTCATTCGTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATA
  GCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTG
  GCCCTGNGAAGTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATC
  ATTNTGTCTGACTAGGTGTCCTTCTTAATATTATGGGTGAGGNGGGGGGGNTTTNTTACC
  AACGGGGCACATTTGGAAAAAAACCCTTTGGGCCCCGGGGGTTATTGGGACCCAACTTGG
  GGGGCCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001022 unedited
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTACGAATGAGGCAATTTATTAACCCAGCATGG
  TTTGTTCTAATGCTTCTTGTTGGCAGCTGCCACCTGTCCGGCGATTCTGTCCAGATCTCT
  TTGTCCCTGAGGTGTCAGTTTGCGGCCGCCATCTTGGTCCTTTTCCACCATTTTCAGCCC
  CTCCAGGGCTTGGAGGACCCGGCGGGCCACACTCTTGGAGCCTCGGCTGAAGTGGCTGGG
  CATGACGCCGTTTCTCTGACGTCCCCCATAGATCTTGGTCATGGAGCCAACCCCAGCGCC
  ACCCCGGAGGTACAGGTGCCGCGCTGTGGAAGCAGCTCGCGTGTAGAACCAGTTCTCATC
  GTAGGGAGCAAGCTCTTTGTGCTTGGCCAGCTTGACGGTATCCACCCATTCGGGGACTTT
  CAGCTTCCCGGACTTTTTGAGGAAGGCTGCCAGAGCTCTGACGAACTCCTGCTGGTTCAC
  GTCTTTTACAGTAACTCCAGGCATCGTGCGCCCTCCGCGCTGCCAGCCAGGGGAAAGGGA
  ACGACGGGGTTTTCCGGGCGCACCCTCGTGCCGATTCGGCACGAGGCCTCGTGCCGAATT
  CCGGCCGCCCTATGTGAGTCTATTACAAATTCTGACGGTTACTAACCAGCTCTGTTATTA
  GACTCCCACCGACCCGCTACCGCCTTTTGCTTAACGGGCGGGGTTTTACCACTTCTGGAA
  GCCCGTTGTTTGGGGCAAAACAAATCCCTTGGCCTATGGGGGGGGACTTGGAAACCCGTG
  GTTCAACGTTTCCCCCCTTGGGGCCGGCAAACCCTTCCCTGGGAAACGCGCCCATCCCTA
  ATCCCCCCCTCGAAAACCCCCAGGGCTTCCGGCATTTGCACGGGCTTTTCGTTTTGCCCA
  AGGGGCCCCTGTTTTATACTTTTTCTCCAGGGCTTCCCGTACCTCCCTTGCTTGGAGTCC
  TGCGGCTCGGACTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001022
• Insert Size: 450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001022.3, NP_001013.1
• RefSeq Size: 872 bp
• RefSeq ORF: 438 bp
• Locus ID: 6223
• Cytogenetics: 19q13.2
• Domains: Ribosomal_S19e
• Protein Families: Druggable Genome
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'