RPS20 (NM_001023) Human Untagged Clone

CAT#: SC119531

RPS20 (untagged)-Human ribosomal protein S20 (RPS20), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS20
• Synonyms: S20; uS10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119531 sequence for NM_001023 edited (data generated by NextGen Sequencing)
  ATGGCTTTTAAGGATACCGGAAAAACACCCGTGGAGCCGGAGGTGGCAATTCACCGAATT
  CGAATCACCCTAACAAGCCGCAACGTAAAATCCTTGGAAAAGGTGTGTGCTGACTTGATA
  AGAGGCGCAAAAGAAAAGAATCTCAAAGTGAAAGGACCAGTTCGAATGCCTACCAAGACT
  TTGAGAATCACTACAAGAAAAACTCCTTGTGGTGAAGGTTCTAAGACGTGGGATCGTTTC
  CAGATGAGAATTCACAAGCGACTCATTGACTTGCACAGTCCTTCTGAGATTGTTAAGCAG
  ATTACTTCCATCAGTATTGAGCCAGGAGTTGAGGTGGAAGTCACCATTGCAGATGCTTAA
  
  Clone variation with respect to NM_001023.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001023 unedited
  GTCATATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCGCGGTCGTAA
  GGGCTGAGGATTTTTGGTCCGCACGCTCCTGCTCCTGACTCACCGCTGTTCGCTCTCGCC
  GAGGAACAAGTCGGTCAGGAAGCCCGCGCGCAACAGCCATGGCTTTTAAGGATACCGGAA
  AAACACCCGTGGAGCCGGAGGTGGCAATTCACCGAATTCGAATCACCCTAACAAGCCGCA
  ACGTAAAATCCTTGGAAAAGGTGTGTGCTGACTTGATAAGAGGCGCAAAAGAAAAGAATC
  TCAAAGTGAAAGGACCAGTTCGAATGCCTACCAAGACTTTGAGAATCACTACAAGAAAAA
  CTCCTTGTGGTGAAGGTTCTAAGACGTGGGATCGTTTCCAGATGAGAATTCACAAGCGAC
  TCATTGACTTGCACAGTCCTTCTGAGATTGTTAAGCAGATTACTTCCATCAGTATTGAGC
  CAGGAGTTGAGGTGGAAGTCACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGA
  TGACCAGTTGTTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAG
  CTGTTTCCTGAACAGACTCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTTG
  CCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATAAGTTGCATTAT
  TTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAAGCGGGTGGTATGGGAACC
  ACGGGTCCATCTGCGCTAAAAACCCTTTTACGTGCCTGTCGGGGCTATCTGGGAACTCAT
  TCCTGAATGGCAACGGTCCAACATTGGCTTAATTGGATTTTTTCCTCCTTTTGGTCTTAA
  GCGAATTCTCCTGCCTTTACACTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001023 unedited
  CGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAACAACTGGTCATCAATTTA
  TTAAAATAGTTGACTTAAGCATCTGCAATGGTGACTTCCACCTCAACTCCTGGCTCAATA
  CTGATGGAAGTAATCTGCTTAACAATCTCAGAAGGACTGTGCAAGTCAATGAGTCGCTTG
  TGAATTCTCATCTGGAAACGATCCCACGTCTTATAACCTTCACCACAAGGAGTTTTTCTT
  GTAGTGATTCTCAAAGTCTTGGTAGGCATTCGAACTGGTCCTTTCACTTTGAGATTCTTT
  TCTTTTGCGCCTCTTATCAAGTCAGCACACACCTTTTCCAAGGATTTTACGTTGCGGCTT
  GTTAGGGTGATTCGAATTCGGTGAATTGCCACCTCCGGCTCCACGGGTGTTTTTCCGGTA
  TCCTTAAAAGCCATGGCTGTTGCGCGCGGGCTTCCTGACCGACTTGTTCCTCGGCGAGAG
  CGAACAGCGGTGAGTCAGGATCAGGAGCGTGCGGACCAAAAATCCTCAGCCCTTACGACC
  GCGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGC
  TCACTAAACGAGCTCTGCTTATATAGACCTCCCACCCGTACACGCCTACCGCCCATTTGC
  GTCAACGGAGCGGGGTTATTACGACATTTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAA
  CAAACTCCCATTGACGTCAATGGNGTGGAGACTCGGGAATTCCCGTGAGTCAAACCGCTA
  TCCACGCCCATTGGTGTACTGTCAAAACCGCATCACCATGTGTATAGCGATGACTAATAC
  CTAGATGTACTGCCCAGTAAGAAAGCCCCGTAAAGTCATGTACTGGGCTTAATGCCGGCG
  GGCCATTTACCTCATTGACGTTATAAGGGCCGGACTTGCCTATGATCCCTTGAGTACTGC
  CAGTGGGCAGTTACAGTAATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001023
• Insert Size: 530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001023.2, NP_001014.1
• RefSeq Size: 539 bp
• RefSeq ORF: 360 bp
• Locus ID: 6224
• Cytogenetics: 8q12.1
• Domains: Ribosomal_S10
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]'
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1.