RPS21 (NM_001024) Human Untagged Clone

CAT#: SC119532

RPS21 (untagged)-Human ribosomal protein S21 (RPS21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS21
• Synonyms: HLDF; S21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119532 sequence for NM_001024 edited (data generated by NextGen Sequencing)
  ATGCAGAACGACGCCGGCGAGTTCGTGGACCTGTACGTGCCGCGGAAATGCTCCGCTAGC
  AATCGCATCATCGGTGCCAAGGACCACGCATCCATCCAGATGAACGTGGCCGAGGTTGAC
  AAGGTCACAGGCAGGTTTAATGGCCAGTTTAAAACTTATGCTATCTGCGGGGCCATTCGT
  AGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCA
  AAGAACTTTTGA
  
  Clone variation with respect to NM_001024.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001024 unedited
  AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGCAGGCGCAGC
  CCAGCCTCGAAATGCAGAACGACGCCGGCGAGTTCGTGGACCTGTACGTGCCGCGGAAAT
  GCTCCGCTAGCAATCGCATCATCGGTGCCAAGGACCACGCATCCATCCAGATGAACGTGG
  CCGAGGTTGACAAGGTCACAGGCAGGTTTAATGGCCAGTTTAAAACTTATGCTATCTGCG
  GGGCCATTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATG
  GCATCGTCTCAAAGAACTTTTGACTGGAGAGAATCACAGATGTGGAATATTTGTCATAAA
  TAAATAATGAAAACCTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTC
  ATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTC
  CTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGC
  ATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGGTATG
  GAACCCAAGGGGCAAGNTGGGAAGACAACCTGTAGGGCCTGCCGNGGTCTATTGGGAACC
  AAGCTGGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTTCTGGGTTCAAG
  CGAATCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCCAGGCATGCTTGACCCAGCTT
  CACTAATTTTTGTTTTTTTGTAAAAACGGGGTTCACCATATTGGCCAGCCTGGGCTCCAC
  CTCTAATTTAGGTGAACACCCCCCTAGCCCCCCCAAATGCTGGGAATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001024 unedited
  GGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGTTTTCATTATTTA
  TTTATGACAAATATTCCACATCTGTGATTCTCTCCAGTCAAAAGTTCTTTGAGACGATGC
  CATCGGCCTTGGCCAATCGGAGAATGGAATCATCTGACTCACCCATCCTACGAATGGCCC
  CGCAGATAGCATAAGTTTTAAACTGGCCATTAAACCTGCCTGTGACCTTGTCAACCTCGG
  CCACGTTCATCTGGATGGATGCGTGGTCCTTGGCACCGATGATGCGATTGCTAGCGGAGC
  ATTTCCGCGGCACGTACAGGTCCACGAACTCGCCGGCGTCGTTCTGCATTTCGAGGCTGG
  GCTGCGCCTGCTCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAAT
  TCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCCGTACACGCCTACCG
  CCCATTTGTGTCAACGGGGCGGGGTTATTTACGACATTTTGGAAAGTCCCGTTGATTTTG
  GTGCCAAAACAAACTCCCATGGACGTCAATGGTGTGGAGACTTGGAAATCCCCGTGAGCC
  AAACCGCTATCCACGCCCATTGGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGAT
  GACTAATACGTAGATGTCCTGCCAAGTAGGAAGTCCCGTAAGGCCATGTACCGGGCATAA
  TGCCCGGCGGGCCATTTACCGTATTGACCTCATAGGGGGCGGACTTGCCTTTGACCCCTG
  ATGTACTGCCAGGGGCATTCCCCCGATCACCTCCCCCCTTGCCCCCATGGAAAGCCCTAT
  TGCGTACCATGGAACCTACGCCATTTCGACTCCACGGCGCGGGTCTTGGCCGGTATCCAC
  CCCGCCTTTTACCTAATCCCTTACCCGGTCCCCCCTACTGGCCTTTACACATCCCCCCGT
  ATGTCCACCTCTAACCCCTACACACCCGTCCCATCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001024
• Insert Size: 340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024.3, NP_001015.1
• RefSeq Size: 418 bp
• RefSeq ORF: 252 bp
• Locus ID: 6227
• Cytogenetics: 20q13.33
• Domains: Ribosomal_S21e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S21E family of ribosomal proteins. It is located in the cytoplasm. Alternative splice variants that encode different protein isoforms have been described, but their existence has not been verified. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'