MPS1 (RPS27) (NM_001030) Human Untagged Clone

CAT#: SC119535

RPS27 (untagged)-Human ribosomal protein S27 (RPS27)


  "NM_001030" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RPS27
Synonyms DBA17; MPS-1; MPS1; S27
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119535 sequence for NM_001030 edited (data generated by NextGen Sequencing)
ATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACAAG
AAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGC
TATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCC
ACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGG
AGGAAGCAGCACTAA

Clone variation with respect to NM_001030.4
>OriGene 5' read for NM_001030 unedited
TTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGTACGCACACGAGA
ACATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACA
AGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGAT
GCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCT
CCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCA
GGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAAC
ACATTTTGGATAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGC
TGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGC
CCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCAT
TTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGAGGGGGGTTGGTATGGGACAA
GGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGA
GTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCC
TGCCTCAGCCTNCCCGAGTTGGTGGGGATTTCAGGCATGCATGACCAGGCTCAGCTATTT
TTTGTTTTTTTGGAGAGAACCGGGGTTTCACCATATGGCCAGGCCTGGTCTCCACTCCTA
TCCTCAGTGATCTACCCACCTGGCCTCCCAATGTTGGGATTACAGCCTG
>OriGene 3' read for NM_001030 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATCCAAAATGTGTTTATTG
AGATGGTTTCCCACTCATCTTGACTCAGAGTGCTTTTAGTGCTGCTTCCTCCTGAAGGAA
CATCCTTCTGTAAGCCTTGCTTTTCCTCCTGTAGGCTGGCAGAGGACAGTGGAGCAGCCA
ACACACAAAACTACCGTTTGTGCATGGCTAAAGACCGTGGTGATTTTATAGCATCCTGGG
CATTTCACATCCATGAAGTAGGAATTGGGGCTCTGCACCAGGCGTTTCTTCTTGTGTTTC
CTCTTCTCCTCTTCTGGAGAGGGATGAAGGAGATCCTTTGCGAGAGGCATGTTCTCGTGT
GCGTACCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACG
GTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTG
CGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAA
CAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTA
TCCACGCCCATTGGTGTACTGCCAAAACCGCATTACCATGGTTATAGCGATGACTAATAC
CTACATGTCCTGCCAAAGAAGAAAGTCCCGGAAGGGCATGTACTGGGCATAATCCCGGGC
GGCCCTTTACCGGAATTGCGTCAATAGGGGCCGACTTGCCTAGATCCACTAGAGTACCGC
CACGGGCCAGTTACCGCAAAACCCCCCCATCGCCCAATGGAAAGCCCTTTGGCCGTCCTT
GGGAACCTCCTCATTTTGCCNCATGGCCGGGGTCTTGGGCGNCACCCGCGGGCCTTTCCG
ATAGTTTTACCCCACCCTCTATAGCCTAAACCAAGCCCCCTATGT
Restriction Sites NotI-NotI     
ACCN NM_001030
Insert Size 400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001030.3, NP_001021.1
RefSeq Size 372 bp
RefSeq ORF 255 bp
Locus ID 6232
Cytogenetics 1q21.3
Domains Ribosomal_S27e
Protein Pathways Ribosome
Gene Summary 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2018]'
Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1).

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