MPS1 (RPS27) (NM_001030) Human Untagged Clone

CAT#: SC119535

RPS27 (untagged)-Human ribosomal protein S27 (RPS27)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS27
• Synonyms: DBA17; MPS-1; MPS1; S27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119535 sequence for NM_001030 edited (data generated by NextGen Sequencing)
  ATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACAAG
  AAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGC
  TATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCC
  ACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGG
  AGGAAGCAGCACTAA
  
  Clone variation with respect to NM_001030.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001030 unedited
  TTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGTACGCACACGAGA
  ACATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACA
  AGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGAT
  GCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCT
  CCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCA
  GGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAAC
  ACATTTTGGATAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGC
  TGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGC
  CCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCAT
  TTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGAGGGGGGTTGGTATGGGACAA
  GGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGA
  GTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCC
  TGCCTCAGCCTNCCCGAGTTGGTGGGGATTTCAGGCATGCATGACCAGGCTCAGCTATTT
  TTTGTTTTTTTGGAGAGAACCGGGGTTTCACCATATGGCCAGGCCTGGTCTCCACTCCTA
  TCCTCAGTGATCTACCCACCTGGCCTCCCAATGTTGGGATTACAGCCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001030 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATCCAAAATGTGTTTATTG
  AGATGGTTTCCCACTCATCTTGACTCAGAGTGCTTTTAGTGCTGCTTCCTCCTGAAGGAA
  CATCCTTCTGTAAGCCTTGCTTTTCCTCCTGTAGGCTGGCAGAGGACAGTGGAGCAGCCA
  ACACACAAAACTACCGTTTGTGCATGGCTAAAGACCGTGGTGATTTTATAGCATCCTGGG
  CATTTCACATCCATGAAGTAGGAATTGGGGCTCTGCACCAGGCGTTTCTTCTTGTGTTTC
  CTCTTCTCCTCTTCTGGAGAGGGATGAAGGAGATCCTTTGCGAGAGGCATGTTCTCGTGT
  GCGTACCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACG
  GTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTG
  CGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAA
  CAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTA
  TCCACGCCCATTGGTGTACTGCCAAAACCGCATTACCATGGTTATAGCGATGACTAATAC
  CTACATGTCCTGCCAAAGAAGAAAGTCCCGGAAGGGCATGTACTGGGCATAATCCCGGGC
  GGCCCTTTACCGGAATTGCGTCAATAGGGGCCGACTTGCCTAGATCCACTAGAGTACCGC
  CACGGGCCAGTTACCGCAAAACCCCCCCATCGCCCAATGGAAAGCCCTTTGGCCGTCCTT
  GGGAACCTCCTCATTTTGCCNCATGGCCGGGGTCTTGGGCGNCACCCGCGGGCCTTTCCG
  ATAGTTTTACCCCACCCTCTATAGCCTAAACCAAGCCCCCTATGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001030
• Insert Size: 400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030.3, NP_001021.1
• RefSeq Size: 372 bp
• RefSeq ORF: 255 bp
• Locus ID: 6232
• Cytogenetics: 1q21.3
• Domains: Ribosomal_S27e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2018]'
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1).