RRM2 (NM_001034) Human Untagged Clone

CAT#: SC119538

RRM2 (untagged)-Human ribonucleotide reductase M2 (RRM2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RRM2
• Synonyms: C2orf48; R2; RR2; RR2M
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119538 sequence for NM_001034 edited (data generated by NextGen Sequencing)
  ATGCTCTCCCTCCGTGTCCCGCTCGCGCCCATCACGGACCCGCAGCAGCTGCAGCTCTCG
  CCGCTGAAGGGGCTCAGCTTGGTCGACAAGGAGAACACGCCGCCGGCCCTGAGCGGGACC
  CGCGTCCTGGCCAGCAAGACCGCGAGGAGGATCTTCCAGGAGCCCACGGAGCCGAAAACT
  AAAGCAGCTGCCCCCGGCGTGGAGGATGAGCCGCTGCTGAGAGAAAACCCCCGCCGCTTT
  GTCATCTTCCCCATCGAGTACCATGATATCTGGCAGATGTATAAGAAGGCAGAGGCTTCC
  TTTTGGACCGCCGAGGAGGTGGACCTCTCCAAGGACATTCAGCACTGGGAATCCCTGAAA
  CCCGAGGAGAGATATTTTATATCCCATGTTCTGGCTTTCTTTGCAGCAAGCGATGGCATA
  GTAAATGAAAACTTGGTGGAGCGATTTAGCCAAGAAGTTCAGATTACAGAAGCCCGCTGT
  TTCTATGGCTTCCAAATTGCCATGGAAAACATACATTCTGAAATGTATAGTCTTCTTATT
  GACACTTACATAAAAGATCCCAAAGAAAGGGAATTTCTCTTCAATGCCATTGAAACGATG
  CCTTGTGTCAAGAAGAAGGCAGACTGGGCCTTGCGCTGGATTGGGGACAAAGAGGCTACC
  TATGGTGAACGTGTTGTAGCCTTTGCTGCAGTGGAAGGCATTTTCTTTTCCGGTTCTTTT
  GCGTCGATATTCTGGCTCAAGAAACGAGGACTGATGCCTGGCCTCACATTTTCTAATGAA
  CTTATTAGCAGAGATGAGGGTTTACACTGTGATTTTGCTTGCCTGATGTTCAAACACCTG
  GTACACAAACCATCGGAGGAGAGAGTAAGAGAAATAATTATCAATGCTGTTCGGATAGAA
  CAGGAGTTCCTCACTGAGGCCTTGCCTGTGAAGCTCATTGGGATGAATTGCACTCTAATG
  AAGCAATACATTGAGTTTGTGGCAGACAGACTTATGCTGGAACTGGGTTTTAGCAAGGTT
  TTCAGAGTAGAGAACCCATTTGACTTTATGGAGAATATTTCACTGGAAGGAAAGACTAAC
  TTCTTTGAGAAGAGAGTAGGCGAGTATCAGAGGATGGGAGTGATGTCAAGTCCAACAGAG
  AATTCTTTTACCTTGGATGCTGACTTCTAA
  
  Clone variation with respect to NM_001034.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001034 unedited
  NTTGTCANAATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGTCCTGTC
  CTGGCTGCTCGCTCTGCTTCGCTGCGCCTCCCTATGCTCTCCCTCCGTGTCCCGCTCGCG
  CCCATCACGGACCCGCAGCAGCTGCAGCTCTCGCCGCTGAAGGGGCTCAGCTTGGTCGAC
  AAGGAGAACACGCCGCCGGCCCTGAGCGGGACCCGCGTCCTGGCCAGCAAGACCGCGAGG
  AGGATCTTCCAGGAGCCCACGGAGCCGAAAACTAAAGCAGCTGCCCCCGGCGTGGAGGAT
  GAGCCGCTGCTGAGAGAAAACCCCCGCCGCTTTGTCATCTTCCCCATCGAGTACCATGAT
  ATCTGGCAGATGTATAAGAAGGCAGAGGCTTCCTTTTGGACCGCCGAGGAGGTGGACCTC
  TCCAAGGACATTCAGCACTGGGAATCCCTGAAACCCGAGGAGAGATATTTTATATCCCAT
  GTTCTGGCTTTCTTTGCAGCAAGCGATGGCATAGTAAATGAAAACTTGGTGGAGCGATTT
  AGCCAAGAAGTTCAGATTACAGAAGCCCGCTGTTTCTATGGCTTCCAAATTGCCATGGAA
  AACATACATTCTGAAATGTATAGTCTTCTTATTGACACTTACATAAAAGATCCCAAAGAA
  AGGGAATTTCTCTTCAATGCCATTGAAACGATGCCTTGTGTCAAGAAGAAGGCAGACTGG
  GCCTTGCGCTGGATTGGGGACANAGAGGCTACCTATGGTGAACGTNGTGTANCCTTTGCT
  GCAGTGGAAGGCATTNTCTTTTCCGGNTCTTTTGCGTCGATATTCTGGCTCAGAAACGAG
  GACTGATGCCTGGCCTCACATTNTCTAATGAACTATTAGCAGAGATGAGGTTTACACTGT
  GATTTTGCTTGCCTGATGNTCAACACCTGGTACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001034 unedited
  GGACTACTTGNCCGCGGCCGCNGTCNANGATCGGTTTTTTTTTTTTTTTTTTGGTTAACA
  AATATTTTAATTCTTAAATAAAACTTAAAAATTACATGCTTAGTCTACACAAGTTTAACT
  TACTTTAGTCACTTAGTGAATTGTGAATTGGCTCCCATTAGTGGTCAGGAGAATGTATTT
  GGTGTAGAAACCAAATAAATCAAGCTATTATCGCCTTGTGAGTACAAACAATGTTTATTT
  GTTTGTAAAGTGCCAGGTTTATATTTAAGTAAACATTAAAATCTGCGTTGAAGCAGTGAG
  GCTGCATCTTTTAACTGGCTGTGCTGGTTAAAGGACTGTTTAATCCCGCTATGCTAAGCT
  CACTAAAGGGTCACCCCTCACTTTAAAGCCAAGACTGCCATTGTCACTGCTATGGTAAGT
  CACAGCCAGCCAGGCCTTCTGGCAAAAGGTGATACTACCAGCACTATAAACAGACAGGAC
  TGGTTGTGAGGTAGCTACACAGTTTTAAAGATGCTGTTAATGAACATTACGGACAATTCA
  TGGTGTGGCTAGTTGGTAACACTTCAGCTGATTTTTCTTATGAGATGGAAAAAAAAAATC
  AGCCAAGTAAGGGCACATCTTCAGTTCATTTAGAAGTCAGCATCCAAGGTAAAAGAATTC
  TCTGTTGGACTTGACATCACTCCCATCCTCTGATACTCGCCTACTCTCTTCTCAAAGAAG
  TTAGTCCTTCCTTCCAGNGAAATATTCTCCATAAAGTCAATGGGTTCTCTACTCTGAAAA
  CCTGCTAAACCCAGTNNCAGCATAGTCTGTCTGCACAAACTCATGTATTGCTTCATAGAG
  TGCATTCATCCCATGAGCTTCACAGCAAGGGCTCATGAGGGACTCCTGTTCTATTCGACA
  GCATTGAAAATATTTCTCTTACTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001034
• Insert Size: 1770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034.1, NP_001025.1
• RefSeq Size: 2500 bp
• RefSeq ORF: 1170 bp
• Locus ID: 6241
• Cytogenetics: 2p25.1
• Domains: ribonuc_red_sm
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, p53 signaling pathway, Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) uses an alternative downstream promoter, and it thus has a shorter 5' UTR and uses a downstream translational start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. CCDS Note: This CCDS represents the transcript derived from the downstream promoter of this gene. Studies in both PMIDs 10631117 and 11978970 support the presence of the downstream promoter, as do the 5' extents of several human and homologous transcripts. PMID:11978970 also provides evidence for an upstream promoter; the transcript derived from the upstream promoter would encode a protein that is 60 aa longer at the N-terminus.