epithelial Sodium Channel alpha (SCNN1A) (NM_001038) Human Untagged Clone

CAT#: SC119542

SCNN1A (untagged)-Human sodium channel, nonvoltage-gated 1 alpha (SCNN1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCNN1A
• Synonyms: BESC2; ENaCa; ENaCalpha; LIDLS3; SCNEA; SCNN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119542 sequence for NM_001038 edited (data generated by NextGen Sequencing)
  ATGGAGGGGAACAAGCTGGAGGAGCAGGACTCTAGCCCTCCACAGTCCACTCCAGGGCTC
  ATGAAGGGGAACAAGCGTGAGGAGCAGGGGCTGGGCCCCGAACCTGCGGCGCCCCAGCAG
  CCCACGGCGGAGGAGGAGGCCCTGATCGAGTTCCACCGCTCCTACCGAGAGCTCTTCGAG
  TTCTTCTGCAACAACACCACCATCCACGGCGCCATCCGCCTGGTGTGCTCCCAGCACAAC
  CGCATGAAGACGGCCTTCTGGGCAGTGCTGTGGCTCTGCACCTTTGGCATGATGTACTGG
  CAATTCGGCCTGCTTTTCGGAGAGTACTTCAGCTACCCCGTCAGCCTCAACATCAACCTC
  AACTCGGACAAGCTCGTCTTCCCCGCAGTGACCATCTGCACCCTCAATCCCTACAGGTAC
  CCGGAAATTAAAGAGGAGCTGGAGGAGCTGGACCGCATCACAGAGCAGACGCTCTTTGAC
  CTGTACAAATACAGCTCCTTCACCACTCTCGTGGCCGGCTCCCGCAGCCGTCGCGACCTG
  CGGGGGACTCTGCCGCACCCCTTGCAGCGCCTGAGGGTCCCGCCCCCGCCTCACGGGGCC
  CGTCGAGCCCGTAGCGTGGCCTCCAGCTTGCGGGACAACAACCCCCAGGTGGACTGGAAG
  GACTGGAAGATCGGCTTCCAGCTGTGCAACCAGAACAAATCGGACTGCTTCTACCAGACA
  TACTCATCAGGGGTGGATGCGGTGAGGGAGTGGTACCGCTTCCACTACATCAACATCCTG
  TCGAGGCTGCCAGAGACTCTGCCATCCCTGGAGGAGGACACGCTGGGCAACTTCATCTTC
  GCCTGCCGCTTCAACCAGGTCTCCTGCAACCAGGCGAATTACTCTCACTTCCACCACCCG
  ATGTATGGAAACTGCTATACTTTCAATGACAAGAACAACTCCAACCTCTGGATGTCTTCC
  ATGCCTGGAATCAACAACGGTCTGTCCCTGATGCTGCGCGCAGAGCAGAATGACTTCATT
  CCCCTGCTGTCCACAGTGACTGGGGCCCGGGTAATGGTGCACGGGCAGGATGAACCTGCC
  TTTATGGATGATGGTGGCTTTAACTTGCGGCCTGGCGTGGAGACCTCCATCAGCATGAGG
  AAGGAAACCCTGGACAGACTTGGGGGCGATTATGGCGACTGCACCAAGAATGGCAGTGAT
  GTTCCTGTTGAGAACCTTTACCCTTCAAAGTACACACAGCAGGTGTGTATTCACTCCTGC
  TTCCAGGAGAGCATGATCAAGGAGTGTGGCTGTGCCTACATCTTCTATCCGCGGCCCCAG
  AACGTGGAGTACTGTGACTACAGAAAGCACAGTTCCTGGGGGTACTGCTACTATAAGCTC
  CAGGTTGACTTCTCCTCAGACCACCTGGGCTGTTTCACCAAGTGCCGGAAGCCATGCAGC
  GTGACCAGCTACCAGCTCTCTGCTGGTTACTCACGATGGCCCTCGGTGACATCCCAGGAA
  TGGGTCTTCCAGATGCTATCGCGACAGAACAATTACACCGTCAACAACAAGAGAAATGGA
  GTGGCCAAAGTCAACATCTTCTTCAAGGAGCTGAACTACAAAACCAATTCTGAGTCTCCC
  TCTGTCACGATGGTCACCCTCCTGTCCAACCTGGGCAGCCAGTGGAGCCTGTGGTTCGGC
  TCCTCGGTGTTGTCTGTGGTGGAGATGGCTGAGCTCGTCTTTGACCTGCTGGTCATCATG
  TTCCTCATGCTGCTCCGAAGGTTCCGAAGCCGATACTGGTCTCCAGGCCGAGGGGGCAGG
  GGTGCTCAGGAGGTAGCCTCCACCCTGGCATCCTCCCCTCCTTCCCACTTCTGCCCCCAC
  CCCATGTCTCTGTCCTTGTCCCAGCCAGGCCCTGCTCCCTCTCCAGCCTTGACAGCCCCT
  CCCCCTGCCTATGCCACCCTGGGCCCCCGCCCATCTCCAGGGGGCTCTGCAGGGGCCAGT
  TCCTCCGCCTGTCCTCTGGGGGGGCCCTGA
  
  Clone variation with respect to NM_001038.5
  1987 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001038 unedited
  NTGTCAGNATTTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACCAGGGGACATGG
  GCATGGCCAGGGGCAGCCTCACTCGGGTTCCAGGGGTGATGGGAGAGGGCACTCAGGGCC
  CAGAGCTCAGCCTTGACCCTGACCCTTGCTCTCCCCAATCCACTCCGGGGCTCATGAAGG
  GGAACAAGCTGGAGGAGCAGGACCCTAGACCTCTGCAGCCCATACCAGGTCTCATGGAGG
  GGAACAAGCTGGAGGAGCAGGACTCTAGCCCTCCACAGTCCACTCCAGGGCTCATGAAGG
  GGAACAAGCGTGAGGAGCAGGGGCTGGGCCCCGAACCTGCGGCGCCCCAGCAGCCCACGG
  CGGAGGAGGAGGCCCTGATCGAGTTCCACCGCTCCTACCGAGAGCTCTTCGAGTTCTTCT
  GCAACAACACCACCATCCACGGCGCCATCCGCCTGGTGTGCTCCCAGCACAACCGCATGA
  AGACGGCCTTCTGGGCAGTGCTGTGGCTCTGCACCTTTGGCATGATGTACTGGCAATTCG
  GCCTGCTTTTCGGAGAGTACTTCAGCTACCCCGTCAGCCTCAACATCACCCTCAACTCGG
  ACAAGCTCGTCTTCCCCGCAGTGACCATCTGCACCCTCAATCCCTACAGGTACCCGGAAA
  TTAAAGAGGAGCTGGAGGAGCTGGACCGCATCACAGAGCAGACGCTCTTTGACCTGTACA
  ATACAGCTCCTTCACCACTCTCGTGGCCGGCTCCCGCAGCCGTCGCGACCTGCGGNGGAC
  TCTGCCGCACCCCTTGCAGCGCCCTGAGGTCCCGCCCCCGCCTCACGGNGCCCGTCGAGC
  CCGTAACGTGGCCTCCAGCTTGCGGGGACACAACCCCCAGNTNACTGGAAGGACTGGNAG
  ATCCGCTCCAGCTGTGCAACCAGACAAATCGACTGCTCTACCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001038 unedited
  CTATGAACCCGCGGCCGCAATCTAGNGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTCACAGCCAACAAACTTTTATGGAACCTCCTGCCTACTTGCTCCAAGCAGGA
  AGAGGGGCTAAACATGGACCATCCTTAACCGGCCAAAATTCATCCCCTGACCGAAGGGCC
  CTAACAGTTTATTGGGAAAGGGAAACCCAATGGTCCAAAAAAGTTTGGGGCAACACCACC
  CCTCCACCAAATGTTATAGGGGCCTACGGAACCCCAGAAAAAGGCGGAGGGACCAATTTG
  GGGAAAAACAAAATGGGGCAAAACCGTCCACAAAAGAAAGGGGGCCTGACCAGGGTTCTA
  AGGAATCCATAGGATTCCTTGGGCAAAGGGTGGGGAAAACTTTAAGCCTGAGGAAATTCC
  TAACCCAAACTTGTAAAGGTATAAAAAACCCAGGGCAAAGGGGCCGGGGCATGGTTTTGT
  AAGGGCCCGGGTATAAGGCTAAAGGACCCCTGGTCCTGCTTTTACTTACCCGGGCCTGTT
  CAAGCCTAATCCCTCGGGACCCAGGAAAGGGAATTTCCCCACCAACAATGGGCCCTTTGG
  GCTCGGCTCCCCCCACATCTTCACCCCCAGCCCAACTTTCCTTCTCCCCCTCCCCTCCAC
  ACCCTCACTATTCGCATCGTTCCCTCTCCCCCGCCCCTATCTCCTCCCCCCCATACCCCC
  CCTTCCCCCTCTCTTTTCCCCTCTTTTTTTATCCTCACGCTTCCCTCCCCCCCCCATTTT
  TCTCCTACCCCCCCCCCCCCCCTATTCCCCTCTCCTCTTCCCTTTCCTTAATCCCCGCTC
  CCCCCCCATTCTCCCCCCCCTCCCCATCTATGTCAACCTCCACACCCCCCTCCCCCCTCC
  CCCCTTCTTCCCCCTCCCCTCCACCTTCCCCACATCAAATCA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001038
• Insert Size: 3080 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038.4, NP_001029.1
• RefSeq Size: 3171 bp
• RefSeq ORF: 2010 bp
• Locus ID: 6337
• Cytogenetics: 12p13.31
• Domains: ASC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Taste transduction
• Gene Summary: 'Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]'
  Transcript Variant: This variant (1, also known as alpha-ENaC1) represents the predominant transcript, and encodes isoform 1.