PAF Receptor (PTAFR) (NM_000952) Human Untagged Clone

CAT#: SC119557

PTAFR (untagged)-Human platelet-activating factor receptor (PTAFR), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTAFR
• Synonyms: PAFR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119557 sequence for NM_000952 edited (data generated by NextGen Sequencing)
  ATGGAGCCACATGACTCCTCCCACATGGACTCTGAGTTCCGATACACTCTCTTCCCGATT
  GTTTACAGCATCATCTTTGTGCTCGGGGTCATTGCTAATGGCTACGTGCTGTGGGTCTTT
  GCCCGCCTGTACCCTTGCAAGAAATTCAATGAGATAAAGATCTTCATGGTGAACCTCACC
  ATGGCGGACATGCTCTTCTTGATCACCCTGCCACTTTGGATTGTCTACTACCAAAACCAG
  GGCAACTGGATACTCCCCAAATTCCTGTGCAACGTGGCTGGCTGCCTTTTCTTCATCAAC
  ACCTACTGCTCTGTGGCCTTCCTGGGCGTCATCACTTATAACCGCTTCCAGGCAGTAACT
  CGGCCCATCAAGACTGCTCAGGCCAACACCCGCAAGCGTGGCATCTCTTTGTCCTTGGTC
  ATCTGGGTGGCCATTGTGGGAGCTGCATCCTACTTCCTCATCCTGGACTCCACCAACACA
  GTGCCCGACAGTGCTGGCTCAGGCAACGTCACTCGCTGCTTTGAGCATTACGAGAAGGGC
  AGCGTGCCAGTCCTCATCATCCACATCTTCATCGTGTTCAGCTTCTTCCTGGTCTTCCTC
  ATCATCCTCTTCTGCAACCTGGTCATCATCCGTACCTTGCTCATGCAGCCGGTGCAGCAG
  CAGCGCAACGCTGAAGTCAAGCGCCGGGCGCTGTGGATGGTGTGCACGGTCTTGGCGGTG
  TTCATCATCTGCTTCGTGCCCCACCACGTGGTGCAGCTGCCCTGGACCCTTGCTGAGCTG
  GGCTTCCAGGACAGCAAATTCCACCAGGCCATTAATGATGCACATCAGGTCACCCTCTGC
  CTCCTTAGCACCAACTGTGTCTTAGACCCTGTTATCTACTGTTTCCTCACCAAGAAGTTC
  CGCAAGCACCTCACCGAAAAGTTCTACAGCATGCGCAGTAGCCGGAAATGCTCCCGGGCC
  ACCACGGATACGGTCACTGAAGTGGTTGTGCCATTCAACCAGATCCCTGGCAATTCCCTC
  AAAAATTAG
  
  Clone variation with respect to NM_000952.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000952 unedited
  AGGTAAGGCACCCTGCACCCCGCTTGTACTATNAGGGCGGCCGCGCAATTCGCACCAGCA
  GNACCCATTCGTAGCCACACGGTCACTGCAGCTGAAGCCGCTGCCCCTGCTACAGGCACC
  ACCAGGACCAGCTGATCATTCCAGCCCACAGCAATGGAGCCACATGCCTCCTCCCACAGG
  ACTCTGAGTTCCGATACACTCTCTTCCCGACTGTTTACAGCATCATCTTTGTGCTCGGGG
  TCATTGCTAATGGCTACGTGCTGTGGGTCTTTGCCCGCCTGTACCCTTGCAAGAAATTCA
  ATGAGATAAAGATCTTCATGGCGAACCTCACCATGGCGGACATGCTCTTCTTGATCACCC
  TGCCACTTTGGATTGTCTACTACCAAAACCAGGGCAACTGGATACTCCCCAAATTCCTGT
  GCAACGTGGCTGGCTGCCTTTTCTTCATCAACACCTACTGCTCTGTGGCCTTCCTGGGCG
  TCATCACTTATAACCGCTTCCAGGCAGTAACTCGGCCCATCAAGACTGCTCAGGCCAACA
  CCCGCAAGCGTGGCATCTCTTTGTCCTTGGTCATCTGGGTGGCCATTGTGGGAGCTGCAT
  CCTACTTCCTCATCCTGGACTCCACCAACACAGTGCCCGACAGTGCTGGCTCAGGCCACG
  TCACTCGCTGCTTTGAGCATTACGAGAAGGGCAGCGTGCCAGTCCTCATCATCCACATCT
  TCATCGTGGTCAGCTTCTTCCTGGGCCTTCTCATCATCCTCTTTTGCAACCTGGTCATCA
  TCCGTACCTTGCTCATGCAGCCGGTGCACCAGCAGCGCACCGCTTAAGTTCAGCGCCCGG
  GCGCTGTGGAATGGGTGCCCGTGCTTGGGCGGGGTTCATAATTGCTTCGTGGCCCACCAC
  GGGGGGCAGTGCCCTGGACCCTTGTTGACCTGAGCTTCCAGGAACGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000952 unedited
  GTCTTGGCCGCGGCCGCATCTANAGTCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA
  GATGGAATCTCGCTCTGTTGCCAGGCTAGAGTGCAATGGTGCAATCTAGGTTCACTGCAA
  CCTCTGCCTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTAC
  AGGCGTGTGCCACCATGCCCGGCTAATTTTTGTATGTTTTAGTAGAGATGGGGTTTTACA
  ATGTTGGCCAGGATGGTCTCGATCTCTTGACCCATGATCTGCCCACCTCGGCCTCCCAAA
  GTGCTGGGATTACAGGTGTGAGCCACTGCGCCCACTCCCCCAAAGCTTTAGACCGATGCC
  CCATCGGGGAGAACTAGGTGGTTTAAAGTCTTCCCCCAAGGTCTGCACCTGGCCCTGACA
  TTCCTTCCGGCCCCATAAGATTAAGGGACTCAGGATAAAGTCATCAGTCACAGTTACTGT
  AGTATGCGCCCACAGGCGGATGAAGGGGCTCATTTGAGTTCTGGATTTTCCAACAGCCTG
  GCTCTGCCATCATCCCTGCCCAGGTGAAGTAGCCTCCAAATCTAATGGCCCCCCAGTGCC
  CACAGAGGGGGTGCCCCAGACCACAGTAGATATCCCTTCTTCCCCCCACTCAGTCCAAGA
  TGTTCATGGGAGAGAAGACTTCAGGGCCTGGAAGCAGGGACTTAATTTTGAGGGGATTGC
  CCGGGATCCGGGTGGATGGCACAACCACTTCAGTGACCGGATTCCGGGGGGGCCGGGGAG
  CATTTCGGGCTACTGGGGCAGCTGGAGAAATTTTCGGGGAGGGGCCTGCGGAACTTCTTG
  GGGAGGAACCAGATGATACCGGGTCTTAGAACCAATTGGGTGCTAGGAGGCAAAGGGGGA
  CCCGAGGGCATCATAATGGCCCGTGGGAAATTGCTGCCCGGAACCCAATTAACAGGGGCC
  AGGGCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000952
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000952.3, NP_000943.1
• RefSeq Size: 1822 bp
• RefSeq ORF: 1029 bp
• Locus ID: 5724
• Cytogenetics: 1p35.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (3) lacks the 5' exon, but has an alternate 5' segment, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.