EP1 (PTGER1) (NM_000955) Human Untagged Clone

CAT#: SC119559

PTGER1 (untagged)-Human prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTGER1
• Synonyms: EP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119559 sequence for NM_000955 edited (data generated by NextGen Sequencing)
  ATGAGCCCTTGCGGGCCCCTCAACCTGAGCCTGGCGGGCGAGGCGACCACATGCGCGGCG
  CCCTGGGTCCCCAACACGTCGGCCGTGCCGCCGTCGGGCGCTTCGCCCGCGCTGCCCATC
  TTCTCCATGACGCTGGGCGCCGTGTCCAACCTGCTGGCGCTGGCGCTGCTGGCGCAGGCC
  GCGGGCCGCCTGCGACGCCGCCGCTCGGCCGCCACCTTCCTGCTGTTCGTGGCCAGCCTG
  CTGGCCACCGACCTGGCGGGCCACGTGATCCCGGGCGCGCTGGTGCTGCGTCTGTACACT
  GCGGGGCGCGCTCCGGCCGGCGGGGCCTGCCACTTCCTGGGCGGCTGCATGGTCTTCTTC
  GGCCTGTGCCCGCTGCTGCTGGGCTGTGGCATGGCCGTGGAGCGCTGCGTGGGCGTCACG
  CGGCCGCTGCTCCACGCCGCGCGGGTCTCGGTCGCCCGCGCGCGCCTGGCGCTGGCCGCG
  GTGGCCGCGGTGGCCTTGGCCGTGGCGCTGCTGCCGCTGGCGCGCGTGGGCCGCTATGAG
  CTGCAGTACCCGGGCACGTGGTGCTTCATCGGCCTGGGTCCCCCGGGCGGCTGGCGCCAG
  GCACTGCTTGCTGGCCTCTTCGCCAGCCTCGGCCTGGTCGCGCTCCTCGCCGCGCTGGTG
  TGCAACACGCTCAGCGGCCTGGCCCTGCTACGCGCCCGCTGGCGACGCCGCTCCCGACGG
  CCTCCCCCGGCCTCAGGCCCCGACAGCCGGCGTCGCTGGGGGGCGCACGGACCCCGCTCG
  GCCTCCGCCTCGTCCGCCTCGTCCATCGCTTCGGCCTCCACCTTCTTTGGCGGCTCTCGG
  AGCAGCGGCTCGGCACGCAGAGCTCGCGCCCACGACGTGGAGATGGTGGGCCAGCTTGTC
  GGTATCATGGTGGTGTCGTGCATCTGCTGGAGCCCAATGCTGGTGTTGGTGGCGCTGGCC
  GTCGGCGGCTGGAGCTCTACCTCCCTGCAGCGGCCACTGTTCCTGGCCGTGCGCCTTGCC
  TCCTGGAACCAGATCCTGGACCCTTGGGTGTACATCCTACTGCGCCAGGCCGTGCTGCGC
  CAACTGCTTCGCCTCTTGCCCCCGAGGGCCGGAGCCAAGGGCGGCCCCGCGGGGCTGGGC
  CTAACACCGAGCGCCTGGGAGGCCAGCTCGCTGCGCAGCTCCCGGCACAGCGGCCTCAGC
  CACTTCTAA
  
  Clone variation with respect to NM_000955.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000955 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCAGGGCTGAGC
  GGCCGGTGATGGGGACCCCACATCCCAGGCAGTGCCGGCACCCCTGGCGCCTGACATGAG
  CCCTTGCGGGCCCCTCAACCTGAGCCTGGCGGGCGAGGCGACCACATGCGCGGCGCCCTG
  GGTCCCCAACACGTCGGCCGTGCCGCCGTCGGGCGCTTCGCCCGCGCTGCCCATCTTCTC
  CATGACGCTGGGCGCCGTGTCCAACCTGCTGGCGCTGGCGCTGCTGGCGCAGGCCGCGGG
  CCGCCTGCGACGCCGCCGCTCGGCCGCCACCTTCCTGCTGTTCGTGGCCAGCCTGCTGGC
  CACCGACCTGGCGGGCCACGTGATCCCGGGCGCGCTGGTGCTGCGTCTGTACACTGCGGG
  GCGCGCTCCGGCCGGCGGGGCCTGCCACTTCCTGGGCGGCTGCATGGTCTTCTTCGGCCT
  GTGCCCGCTGCTGCTGGGCTGTGGCATGGCCGTGGAGCGCTGCGTGGGCGTCACGCGGCC
  GCTGCTCCAGCCGCGCGGGTCTCGGTCGCCCGCGCGCGCCTGGCGCTGGCCGCGGTGGCC
  GCGGTGGCCTTGGCCGTGGCGCTGCTGCCGCTGGCGCGCGTGGGCCGCTATGAGCTGCAG
  TACCCGGGCACGTGGTGCTTCATCGGCCTGGGTCCCCCGGGCGGCTGGCGCCAGGCACTG
  CTTGCTGGCCTCTTCGCCAGCCTCGGCCTGGTCGCGCTCCTCGCCGCGCTGGTGTGCAAC
  ACGCTCAGCGGCCTGGCCCTGCTACGCGCCCGCTGGCGACGCCGCTCCCGAAGGCCTCCC
  CCGGCCTCAGGCCCGACAGCCGGGGTCCCTTGGGGGGCGCACGGAACCCGCTCGGGCCTC
  GCCTCGTCCGGCTTGTCCATCGTTTCGGCCTCCAACTTTTTTGGGGGGCTTTCGGGACAA
  CGGGCTGGGAACGCAAAATTTGGCCCACAAAGTGGAAATAGTGGGCCACCTTGGTCGTAT
  AATGGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000955 unedited
  NAAAAATCCTGTGNACGTGGCCGTTTTCTAGGAGCGAGGNATTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTGGGTTTGAAAAGGGTTTTTTATTTCTAAAGGTTTTGGGCCG
  GGCACATGGGCCCAGCCCAGGGTGGGCTGGTTTAGTTGTTGGGCCTTTGGTTGTGTTTAA
  AAGTGGTTGAGGCCGTTGTGCTGGGAGTTGTTTTTTTGTTGGCCTTCAGGCGCTTGGTGT
  TAGGCCCAGCCCCGCGGGGCCGCCCTTGGTTTCGGCCCTTGGGGGCAAGAGGCGAAGCAG
  TTGGTGCAGAACGGTCTGGCGCAGTAGGATGTACACCTCAAGGGTTCAGGATCTGGTTCC
  AGGAGGCAAGGTGCACGGTCAGAAACAGTGGCCGTTGCAGGGAGGTAGAGTTTTAGTCTG
  TTGACGGCCAGTGCCACCAACACCAGTATTGGGTTTTTGTAGAGGCACGACACCTCCTTG
  ATACCGACAAGTTGGTCCAACATTTTCAGGTTGTGGGTGCGAGTTTTGTGTGCCGAGCCG
  TTGTTTCGAGAGCCGTCAAAGAAGGTGGAGGCCGAAAGTGATGGACTAAGTGAACAAGGT
  GGAGGCCGAGCGGGGTCCTGGTGCCCCCCAGTAATCCGGTTTGTTGGATCTTAGGTATGG
  GGAAGGTTGTTGGAAGCGGGGTTTTCCGGGGTTTCTTTAAAGGGGCCAGGCCCTTATGCG
  GGGTTGACACCAACCCGGCATAGGAGCTGAATCAGCCTGAGGTTTGGTAAAAAGGGCTAT
  AAGCAATGTTTGGGTGCCACTCCTCCGGGGGCCCCTGTCCGTTAGAAACACCACGGTCCT
  CGGTATTTGAGTTTATAGGGGGCCACGTTCCCCCCTGTGCCTCCCGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000955
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000955.2, NP_000946.2
• RefSeq Size: 1458 bp
• RefSeq ORF: 1209 bp
• Locus ID: 5731
• Cytogenetics: 19p13.12
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). Through a phosphatidylinositol-calcium second messenger system, G-Q proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin. [provided by RefSeq, Jul 2008]'