EP4 (PTGER4) (NM_000958) Human Untagged Clone

CAT#: SC119561

PTGER4 (untagged)-Human prostaglandin E receptor 4 (subtype EP4) (PTGER4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTGER4
• Synonyms: EP4; EP4R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119561 sequence for NM_000958 edited (data generated by NextGen Sequencing)
  ATGTCCACTCCCGGGGTCAATTCGTCCGCCTCCTTGAGCCCCGACCGGCTGAACAGCCCA
  GTGACCATCCCGGCGGTGATGTTCATCTTCGGGGTGGTGGGCAACCTGGTGGCCATCGTG
  GTGCTGTGCAAGTCGCGCAAGGAGCAGAAGGAGACGACCTTCTACACGCTGGTATGTGGG
  CTGGCTGTCACCGACCTGTTGGGCACTTTGTTGGTGAGCCCGGTGACCATCGCCACGTAC
  ATGAAGGGCCAATGGCCCGGGGGCCAGCCGCTGTGCGAGTACAGCACCTTCATTCTGCTC
  TTCTTCAGCCTGTCCGGCCTCAGCATCATCTGCGCCATGAGTGTCGAGCGCTACCTGGCC
  ATCAACCATGCCTATTTCTACAGCCACTACGTGGACAAGCGATTGGCGGGCCTCACGCTC
  TTTGCAGTCTATGCGTCCAACGTGCTCTTTTGCGCGCTGCCCAACATGGGTCTCGGTAGC
  TCGCGGCTGCAGTACCCAGACACCTGGTGCTTCATCGACTGGACCACCAACGTGACGGCG
  CACGCCGCCTACTCCTACATGTACGCGGGCTTCAGCTCCTTCCTCATTCTCGCCACCGTC
  CTCTGCAACGTGCTTGTGTGCGGCGCGCTGCTCCGCATGCACCGCCAGTTCATGCGCCGC
  ACCTCGCTGGGCACCGAGCAGCACCACGCGGCCGCGGCCGCCTCGGTTGCCTCCCGGGGC
  CACCCCGCTGCCTCCCCAGCCTTGCCGCGCCTCAGCGACTTTCGGCGCCGCCGGAGCTTC
  CGCCGCATCGCGGGCGCCGAGATCCAGATGGTCATCTTACTCATTGCCACCTCCCTGGTG
  GTGCTCATCTGCTCCATCCCGCTCGTGGTGCGAGTATTCGTCAACCAGTTATATCAGCCA
  AGTTTGGAGCGAGAAGTCAGTAAAAATCCAGATTTGCAGGCCATCCGAATTGCTTCTGTG
  AACCCCATCCTAGACCCCTGGATATATATCCTCCTGAGAAAGACAGTGCTCAGTAAAGCA
  ATAGAGAAGATCAAATGCCTCTTCTGCCGCATTGGCGGGTCCCGCAGGGAGCGCTCCGGA
  CAGCACTGCTCAGACAGTCAAAGGACATCTTCTGCCATGTCAGGCCACTCTCGCTCCTTC
  ATCTCCCGGGAGCTGAAGGAGATCAGCAGTACATCTCAGACCCTCCTGCCAGACCTCTCA
  CTGCCAGACCTCAGTGAAAATGGCCTTGGAGGCAGGAATTTGCTTCCAGGTGTGCCTGGC
  ATGGGCCTGGCCCAGGAAGACACCACCTCACTGAGGACTTTGCGAATATCAGAGACCTCA
  GACTCTTCACAGGGTCAGGACTCAGAGAGTGTCTTACTGGTGGATGAGGCTGGTGGGAGC
  GGCAGGGCTGGGCCTGCCCCTAAGGGGAGCTCCCTGCAAGTCACATTTCCCAGTGAAACA
  CTGAACTTATCAGAAAAATGTATATAA
  
  Clone variation with respect to NM_000958.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000958 unedited
  CGCACGGGGGGAGNCGCTACTTTCACNNGGNGGTTCGGAATTGAAACGNATTATATAGGC
  GGACGCCAATTCGCACCAGCCGGCAGCCCGTATAGGAAGATGAACAGCCCCAGGCCAGAG
  CCTCTGGCAGAGTGGACCCCGAGCCGCCCCCAGGTAGCCAGGAGCGGCCTCAGCGGCAGC
  CGCAAACTCCAGTAGCCGCCCGTGCTGCCCGTGGCTGGGGCGGAGGGCAGCCAGAGCTGG
  GGACTTTTTGTTCCGCGCCACCTGCGCGCACAGCCTCACACCTGAACGCTGTCCTCCCGC
  AGACGAGACCGGCGGGCACTGCAAAGCTGGGACTCGTCTTTGAAGGAAAAAAAATAGCGA
  GTAAGAAATCCAGCACCATTCTTCACTGACCCATCCCGCTGCACCTCTTGTTTCCCAAGT
  TTTTGAAAGCTGGCAACTCTGACCTCGGTGTCCAAAAATCGACAGCCACTGAGACCGGCT
  TTGAGAAGCCGAAGATTTGGCAGTTTCCAGACTGAGCAGGACAAGGTGAAAGCAGGTTGG
  AGGCGGGTCCAGGACATCTGAGGGCTGACCCTGNGGGCTCGTGAGGCTGCCACCGCTGCT
  GCCGCTACAGACCCAGCCTTGCACTCCAAGGCTGCGCACCGCCAGCCACTATCATGTCCA
  CTCCCCGGGGTCAATTCGTCCGCCTCCTTGAGCCCCGACCGGCTGAACAGCCCCATGACC
  ATCCCCGCGGGTGATGTTCATCTTCCGGGGTGGGTGGGCAACCTGGTGGGCATCGTGGNG
  GCTGTGCCAAGTCGCGCAAGGAGCCAAAAGGAAACGACCTTTTTACACCCTTGGTATGTG
  GGGCTGGGCTGTCACCGCACCTGTTGGGCCACTTTGTTTGTGGAGCCCGGTGGACCATTG
  CCACGTACATGTAGGGGCCAATGCGCCCGGGGGCCAACCCGTTGGGCCAATCCACACACC
  TTTATTCGGCTCTTCTTAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000958 unedited
  NNTTATTACTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGAT
  TGAATGTATATATTTATTATAATCTCCAAATAATTTCACTTGGTACAACTGCTTCTTAAA
  ACCATATCAATATCAGGCTCAGAATTTAATTACAACCAAGCAATTCACAAAAACACTGAG
  CAACAAAACATGCTTAATATTTCTTTGAGAAAGACCCTTCAAATATGTGTACAGCATCAC
  TGGGAGTTACACAAAACTGTTACAAGGTGACCATTAAGTGCCCCAATTCTGCACTTCTGA
  CATACATGAATGGCTAATGTAACCACGTTTGGGAATCTTTTTACATCTCAAAATAAAGCT
  TTCTGATGCAACTTGCCATCCTTTTAAATTTTAAAGGATATTCTTGGTAATTCCTTAGGA
  AAGTAAAACTACACACACTTTCAGAGAAACAATAAGCTGCTTAGATTTTTAAAATTTTTT
  ATATTATACACTTCAATTATGGGTTATTTATTAAAGACTTCAAAAAATTTATGTTATGTT
  TTTAAAAGTACCAATTAGTGAGGGAAAAAGTTGAAAATATTCACCAGCATGTTAGGTTCT
  CCTGAAATCACAGTTTTCTGGATAAATAAATGTAATATCACTAAACAATTTTCACTATGT
  ATTTTATACATAGCCTGGAAAGAACAGTATTTTGGACTGTAATTTTCACATCTAANATAG
  TATTTTTTTCTTTATCCCTTTGATATACTAANNATCTTCTCTTAAAAATTATATCTATTT
  TATAGATATCAATCTTACTATGAATNTTCAACTTTGACTCGAACTTGNNTATGAAAATAT
  CTAACATCTGCACTTCAGCTGGTATACTGGAAGAAGAGGGTTGCGGATATTTATATACCN
  TGGATAACAATAACCNNTCTACAGAGAGAAATACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000958
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000958.2, NP_000949.1
• RefSeq Size: 3432 bp
• RefSeq ORF: 1467 bp
• Locus ID: 5734
• Cytogenetics: 5p13.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]'