RPL5 (NM_000969) Human Untagged Clone

CAT#: SC119571

RPL5 (untagged)-Human ribosomal protein L5 (RPL5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL5
• Synonyms: L5; MSTP030; PPP1R135; uL18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119571 sequence for NM_000969 edited (data generated by NextGen Sequencing)
  ATGGGGTTTGTTAAAGTTGTTAAGAATAAGGCCTACTTTAAGAGATACCAAGTGAAATTT
  AGAAGACGACGAGAGGGTAAAACTGATTATTATGCTCGGAAACGCTTGGTGATACAAGAT
  AAAAATAAATACAACACACCCAAATACAGGATGATAGTTCGTGTGACAAACAGAGATATC
  ATTTGTCAGATTGCTTATGCCCGTATAGAGGGGGATATGATAGTCTGCGCAGCGTATGCA
  CACGAACTGCCAAAATATGGTGTGAAGGTTGGCCTGACAAATTATGCTGCAGCATATTGT
  ACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGGCATGGACAAGATCTATGAA
  GGCCAAGTGGAGGTGACTGGTGATGAATACAATGTGGAAAGCATTGATGGTCAGCCAGGT
  GCCTTCACCTGCTATTTGGATGCAGGCCTTGCCAGAACTACCACTGGCAATAAAGTTTTT
  GGTGCCCTGAAGGGAGCTGTGGATGGAGGCTTGTCTATCCCTCACAGTACCAAACGATTC
  CCTGGTTATGATTCTGAAAGCAAGGAATTTAATGCAGAAGTACATCGGAAGCACATCATG
  GGCCAGAATGTTGCAGATTACATGCGCTACTTAATGGAAGAAGATGAAGATGCTTACAAG
  AAACAGTTCTCTCAATACATAAAGAACAGCGTAACTCCAGACATGATGGAGGAGATGTAT
  AAGAAAGCTCATGCTGCTATACGAGAGAATCCAGTCTATGAAAAGAAGCCCAAGAAAGAA
  GTTAAAAAGAAGAGGTGGAACCGTCCCAAAATGTCCCTTGCTCAGAAGAAGGATCGGGTA
  GCTCAAAAGAAGGCAAGCTTCCTCAGAGCTCAGGAGCGGGCTGCTGAGAGCTAA
  
  Clone variation with respect to NM_000969.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000969 unedited
  NNNTTGTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCTAGC
  GCCGCTGGGCCTGCAGGTCTCTGTCGAGCAGCGGACGCCGGTCTCTGTTCCGCNAGATGG
  GGTTTGTTAAAGTTGTTAAGAATAAGGCCTACTTTAAGAGATACCAAGTGAAATTTAGAA
  GACGACGAGAGGGTAAAACTGATTATTATGCTCGGAAACGCTTGGTGATACAAGATAAAA
  ATAAATACAACACACCCAAATACAGGATGATAGTTCGTGTGACAAACAGAGATATCATTT
  GTCAGATTGCTTATGCCCGTATAGAGGGGGATATGATAGTCTGCGCAGCGTATGCACACG
  AACTGCCAAAATATGGTGTGAAAGGTTGGCCTGACAAATTATGCTGCAGCATATTGTACT
  GGCCTGCTGCTGGNCCGCAGGCTTCTAATAGGGTTTGGCATGGACAAGATCTATGAAGGC
  CAAGTGGAGGTGACTGGTGATGAATACAATGTGGAAAGATTTGATGGTCAGCCAGGTGCC
  TTCACCTGCTATTTGGATGCANGCCTTGCCAGAACTACCACTGGAATAAAGTTTTTTGGT
  GCCCTGAAGGGAGCTGTGGAATGAAAGCTTTGTNCTATCCTCACAGTACCAAACGATTCC
  CTGGGNNTATGATCCTGAAAGCAAGGGATNTAATGGCAGAAGTACATCGGAAGCACATCA
  TGGGCCCAGATGGTTGCAGATTACATGCGCTACTTAATGGNAAGAGNATTGAGATGCTTA
  CAGGAAACAGTTCTCTCAATACATAAAGAACAGCGTAACTTCGACTGATGGNGGGGGAAA
  GGTTTAAAAAAGCTCATGCTGGTATACGAGAGAATTCAGTCTATGAAAGAAGCCCAGGAA
  AGAGTTAAAAGAAGAGGGGGGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000969 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTGTTCATAAGTTTA
  TTATCTATATCTGAAAAATATAGAAATTGCTGGGTTTAGCTCTCAGCAGCCCGCTCCTGA
  GCTCTGAGGAAGCTTGCCTTCTTTTGAGCTACCCGATCCTTCTTCTGAGCAAGGGACATT
  TTGGGACGGTTCCACCTCTTCTTTTTAACTTCTTTCTTGGGCTTCTTTTCATAGACTGGA
  TTCTCTCGTATAGCAGCATGAGCTTTCTTATACATCTCCTCCATCATGTCTGGAGTTACG
  CTGTTCTTTATGTATTGAGAGAACTGTTTCTTGTAAGCATCTTCATCTTCTTCCATTAAG
  TAGCGCATGTAATCTGCAACATTCTGGCCCATGATGTGCTTCCGATGTACTTCTGCATTA
  AATTCCTTGCTTTCAGAATCATAACCAGGGAATCGTTTGGTACTGTGAGGGATAGACAAG
  CCTCCATCCACAGCTCCCTTCAGGGCACCAAAAACTTTATTGCCAGTGGTAGTTCTGGCA
  AGGCCTGCATCCAAATAGCAGGTGAAGGCACCTGGCTGACCATCAATGCTTTCCACATTG
  TATTCATCACCAGTCACCTCCACTTGGCCTTCATAGATCTTGTCCATGCCAAACCTATTG
  AGAAGCCTGCGGGCCAGCAGCAGGCCAGTACAATATGCTGCAGCATAATTTGTCAGGCCA
  ACCTTCAACCATATTTTGGCAGTTCGTGTGCATACGCTGCGCAGATATCATATCCCCTCT
  ATACGGGCATAAGCATCTGACAATGATATCTCTGTTTGTACACGAACTATATNCTGTATT
  GGGNNGNNGTGNATTATTTTTATCTTGATCACCAGCGTTTCGAGCATATATCAGNTTACC
  NTTTGNCGCTTTAAATTACTNG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000969
• Insert Size: 980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000969.3, NP_000960.2
• RefSeq Size: 1035 bp
• RefSeq ORF: 894 bp
• Locus ID: 6125
• Cytogenetics: 1p22.1
• Domains: Ribosomal_L18p
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. [provided by RefSeq, Mar 2017]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein.