RPL7A (NM_000972) Human Untagged Clone

CAT#: SC119574

RPL7A (untagged)-Human ribosomal protein L7a (RPL7A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL7A
• Synonyms: L7A; SURF3; TRUP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119574 sequence for NM_000972 edited (data generated by NextGen Sequencing)
  ATGCCGAAAGGAAAGAAGGCCAAGGGAAAGAAGGTGGCTCCGGCCCCAGCTGTCGTGAAG
  AAGCAGGAGGCTAAGAAAGTGGTGAATCCCCTGTTTGAGAAAAGGCCTAAGAATTTTGGC
  ATTGGACAGGACATCCAGCCCAAAAGAGACCTCACCCGCTTTGTGAAATGGCCCCGCTAT
  ATCAGGTTGCAGCGGCAGAGAGCCATCCTCTATAAGCGGCTGAAAGTGCCTCCTGCGATT
  AACCAGTTCACCCAGGCCCTGGACCGCCAAACAGCTACTCAGCTGCTTAAGCTGGCCCAC
  AAGTACAGACCAGAGACAAAGCAAGAGAAGAAGCAGAGACTGTTGGCCCGGGCCGAGAAG
  AAGGCTGCTGGCAAAGGGGACGTCCCAACGAAGAGACCACCTGTCCTTCGAGCAGGAGTT
  AACACCGTCACCACCTTGGTGGAGAACAAGAAAGCTCAGCTGGTGGTGATTGCACACGAC
  GTGGATCCCATCGAGCTGGTTGTCTTCTTGCCTGCCCTGTGTCGTAAAATGGGGGTCCCT
  TACTGCATTATCAAGGGAAAGGCAAGACTGGGACGTCTAGTCCACAGGAAGACCTGCACC
  ACTGTCGCCTTCACACAGGTGAACTCGGAAGACAAAGGCGCTTTGGCTAAGCTGGTGGAA
  GCTATCAGGACCAATTACAATGACAGATACGATGAGATCCGCCGTCACTGGGGTGGCAAT
  GTCCTGGGTCCTAAGTCTGTGGCTCGTATCGCCAAGCTCGAAAAGGCAAAGGCTAAAGAA
  CTTGCCACTAAACTGGGTTAA
  
  Clone variation with respect to NM_000972.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000972 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGATGCCGAAAGGAAA
  GAAGGCCAAGGGGAAAGAAGGTGGCTCCGGCCCCAGCTGTCGTGAAGAAGCAGGAGGCTA
  AGAAAGTGGTGAATCCCCTGTTTGAGAAAAGGCCTAAGAATTTTGGCATTGGACAGGACA
  TCCAGCCCAAAAGAGACCTCACCCGCTTTGTGAAATGGCCCCGCTATATCAGGTTGCAGC
  GGCAGAGAGCCATCCTCTATAAGCGGCTGAAAGTGCCTCCTGCGATTAACCAGTTCACCC
  AGGCCCTGGACCGCCAAACAGCTACTCAGCTGCTTAAGCTGGCCCACAAGTACAGACCAG
  AGACAAAGCAAGAGAAGAAGCAGAGACTGTTGGCCCGGGCCGAGAAGAAGGCTGCTGGCA
  AAGGGGACGTCCCAACGAAGAGACCACCTGTCCTTCGAGCAGGAGTTAACACCGTCACCA
  CCTTGGTGGAGAACAAGAAAGCTCAGCTGGTGGTGATTGCACACGACGTGGATCCCATCG
  AGCTGGTTGTCTTCTTGCCTGCCCTGTGTCGTAAAATGGGGGTCCCTTACTGCATTATCA
  AGGGAAAGGCAAGACTGNGACGTCTAGTCCACAGGAAGACCTGCACCACTGTCGCCTTCA
  CACAGGTGAACTCGGAAGACANAGGCGCTTTGGCTAAGCTGGTGGAAGCTATCANGACCA
  ATTACCATGACAGATACGATGAGATCCGCCGTCACTGGGGTGGCAATGTCCCTCGGTCCT
  CAAGTCTGTGGCCTCGTATCGCCCAGCTCCGAAAGGCAAGGCCTAAGAAACTGCCACTAN
  ACTGGGNTAAATGTACCCTGGNGGAATTTTCTGTACCTAAAAATAATGGAATAATACAAA
  ATTTCCTTCAAAAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000972 unedited
  GTACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAAGGAAAATTTGT
  ATTATTTCAATTATTTTTATGTACAGAAAACTCAACAGTGTACATTTAACCCAGTTTAGT
  GGCAAGTTCTTTAGCCTTTGCCTTTTCGAGCTTGGCGATACGAGCCACAGACTTAGGACC
  CAGGACATTGCCACCCCAGTGACGGCGGATCTCATCGTATCTGTCATTGTAATTGGTCCT
  GATAGCTTCCACCAGCTTAGCCAAAGCGCCTTTGTCTTCCGAGTTCACCTGTGTGAAGGC
  GACAGTGGTGCAGGTCTTCCTGTGGACTAGACGTCCCAGTCTTGCCTTTCCCTTGATAAT
  GCAGTAAGGGACCCCCATTTTACGACACAGGGCAGGCAAGAAGACAACCAGCTCGATGGG
  ATCCACGTCGTGTGCAATCACCACCAGCTGAGCTTTCTTGTTCTCCACCAAGGTGGTGAC
  GGTGTTAACTCCTGCTCGAAGGACAGGTGGTCTCTTCGTTGGGACGTCCCCTTTGCCAGC
  AGCCTTCTTCTCGGCCCGGGCCAACAGTCTCTGCTTCTTCTCTTGCTTTGTCTCTGGTCT
  GTACTTGTGGGCCAGCTTAAGCAGCTGAGTAGCTGTTTGGCGGTCCAGGGCCTGGGTGAA
  CTGGTTAATCGCAGGAGGCACTTTCAGCCGCTAATAGAGGATGGCTCTCTGCCGCTGCCA
  CCTGTATAGCGGGGCCATTTCACAAGCGGTTGAGGTCTCTTTTGGGCTGGATGTCCTGTC
  CAATGCCAAATTTTAAGGCTTTTCTCAACAGGGGATTACCACTTTTTTTACCTCTGCTCT
  TCCAGACGCTGGGGCCGGGCCCCTTTTTTCCTTGGGCTCTTTCCTTGGGATTCTCGGGCC
  GATTCCGGCGCCTATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000972
• Insert Size: 930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000972.2, NP_000963.1
• RefSeq Size: 890 bp
• RefSeq ORF: 801 bp
• Locus ID: 6130
• Cytogenetics: 9q34.2
• Domains: Ribosomal_L7Ae
• Protein Families: Druggable Genome
• Protein Pathways: Ribosome
• Gene Summary: 'Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'