IMPDH2 (NM_000884) Human Untagged Clone

CAT#: SC119585

IMPDH2 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMPDH2
• Synonyms: IMPD2; IMPDH-II
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119585 sequence for NM_000884 edited (data generated by NextGen Sequencing)
  ATGGCCGACTACCTGATTAGTGGGGGCACGTCCTACGTGCCAGACGACGGACTCACAGCA
  CAGCAGCTCTTCAACTGCGGAGACGGCCTCACCTACAATGACTTTCTCATTCTCCCTGGG
  TACATCGACTTCACTGCAGACCAGGTGGACCTGACTTCTGCTCTGACCAAGAAAATCACT
  CTTAAGACCCCACTGGTTTCCTCTCCCATGGACACAGTCACAGAGGCTGGGATGGCCATA
  GCAATGGCGCTTACAGGCGGTATTGGCTTCATCCACCACAACTGTACACCTGAATTCCAG
  GCCAATGAAGTTCGGAAAGTGAAGAAATATGAACAGGGATTCATCACAGACCCTGTGGTC
  CTCAGCCCCAAGGATCGCGTGCGGGATGTTTTTGAGGCCAAGGCCCGGCATGGTTTCTGC
  GGTATCCCAATCACAGACACAGGCCGGATGGGGAGCCGCTTGGTGGGCATCATCTCCTCC
  AGGGACATTGATTTTCTCAAAGAGGAGGAACATGACTGTTTCTTGGAAGAGATAATGACA
  AAGAGGGAAGACTTGGTGGTAGCCCCTGCAGGCATCACACTGAAGGAGGCAAATGAAATT
  CTGCAGCGCAGCAAGAAGGGAAAGTTGCCCATTGTAAATGAAGATGATGAGCTTGTGGCC
  ATCATTGCCCGGACAGACCTGAAGAAGAATCGGGACTACCCACTAGCCTCCAAAGATGCC
  AAGAAACAGCTGCTGTGTGGGGCAGCCATTGGCACTCATGAGGATGACAAGTATAGGCTG
  GACTTGCTCGCCCAGGCTGGTGTGGATGTAGTGGTTTTGGACTCTTCCCAGGGAAATTCC
  ATCTTCCAGATCAATATGATCAAGTACATCAAAGACAAATACCCTAATCTCCAAGTCATT
  GGAGGCAATGTGGTCACTGCTGCCCAGGCCAAGAACCTCATTGATGCAGGTGTGGATGCC
  CTGCGGGTGGGCATGGGAAGTGGCTCCATCTGCATTACGCAGGAAGTGCTGGCCTGTGGG
  CGGCCCCAAGCAACAGCAGTGTACAAGGTGTCAGAGTATGCACGGCGCTTTGGTGTTCCG
  GTCATTGCTGATGGAGGAATCCAAAATGTGGGTCATATTGCGAAAGCCTTGGCCCTTGGG
  GCCTCCACAGTCATGATGGGCTCTCTCCTGGCTGCCACCACTGAGGCCCCTGGTGAATAC
  TTCTTTTCCGATGGGATCCGGCTAAAGAAATATCGCGGTATGGGTTCTCTCGATGCCATG
  GACAAGCACCTCAGCAGCCAGAACAGATATTTCAGTGAAGCTGACAAAATCAAAGTGGCC
  CAGGGAGTGTCTGGTGCTGTGCAGGACAAAGGGTCAATCCACAAATTTGTCCCTTACCTG
  ATTGCTGGCATCCAACACTCATGCCAGGACATTGGTGCCAAGAGCTTGACCCAAGTCCGA
  GCCATGATGTACTCTGGGGAGCTTAAGTTTGAGAAGAGAACGTCCTCAGCCCAGGTGGAA
  GGTGGCGTCCATAGCCTCCATTCGTATGAGAAGCGGCTTTTCTGA
  
  Clone variation with respect to NM_000884.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000884 unedited
  CAAGGTTTTGTAAATCCCNACCTCTGAGAGGGCGGCACGAGACTTCGGCACGAGGGTCTC
  TGCGGCGCGGTCCTCGGAGACACGCGGCGGTGTCCTGTGTTGGCCATGGCCGACTACCTG
  ATTAGTGGGGGCACGTCCTACGTGCCAGACGACGGACTCACAGCACAGCAGCTCTTCAAC
  TGCGGAGACGGCCTCACCTACAATGACTTTCTCATTCTTTCTGGGTACATCGACTTCACT
  GCAGACCAGGTGGACCTGACTTCTGCTCTGACCAAGAAAATCACTCTTAAGACCCCACTG
  GTTTCCTCTCCCATGGACACAGTCACAGAGGCTGGGATGGCCATAGCAATGGCGCTTACA
  GGCGGTATTGGCTTCATCCACCACAACTGTACACCTGAATTCCAGGCCAATGAAGTTCGG
  AAAGTGAAGAAATATGAACAGGGATTCATCACAGACCCTGTGGTCCTCAGCCCCAAGGAT
  CGCGTGCGGGATGTTTTTGAGGCCAAGGCCCGGCATGGTTTCTGCGGTATCCCAATCACA
  GACACAGGCCGGATGGGGAGCCGCTTGGTGGGCATCATCTCCTCCAGGGACATTGATTTT
  CTCAAAGAGGAGGAACATGACTGTTTCTTGGAAGAGATAATGACAAAGATGGAAGACTTG
  GTGGTAGCCCCTGCAGGCATCACACTGAAGGATGCAAATGATATTCTGCAGCGCAGCAAG
  AAAGGGAAGTTGCCCATTGGTAATGAAGATGATGAGCTTGTGGCCATCATTGCCCCGACA
  GACCTGGAGAAAATCGGGACTACCCACTAGCCTCCAAAGATGCCAAGAACAGCTGCTGTG
  TGGGGCAGCCATTGGCCCTCATGAAGATGACAAGTATAGGCTTGGACTTGCTCGCCCCAG
  GCCGTTGTTGATAGTATGGTTTTGGACTCTCCCCAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000884 unedited
  NNNNAACACTCTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCT
  TTCTAAACTTTTATTGAAAAAAAAACCGAGGAGGTGTGCTGGATCCCTTTTCAGAAAAGC
  CGCTTCTCATACGAATGGAGGCTATGGACGCCACCTTCCACCTGGGCTGAGGACGTTCTC
  TTCTCAAACTTAAGCTCCCCAGAGTACATCATGGCTCGGACTTGGGTCAAGCTCTTGGCA
  CCAATGTCCTGGCATGAGTGTTGGATGCCAGCAATCAGGTAAGGGACAAATTTGTGGATT
  GACCCTTTGTCCTGCACAGCACCAGACACTCCCTGGGCCACTTTGATTTTGTCAGCTTCA
  CTGAAATATCTGTTCTGGCTGCTGAGGTGCTTGTCCATGGCATCGAGAGAACCCATACCG
  CGATATTTCTTTAGCCGGATCCCATCGGAAAAGAAGTATTCACCAGGGGCCTCAGTGGTG
  GCAGCCAGGAGAGAGCCCATCATGACTGTGGAGGCCCCAAGGGCCAAGGCTTTCGCAATA
  TGACCCACATTTTGGATTCCTCCATCAGCAATGACCGGAACACCAAAGCGCCGTGCATAC
  TCTGACACCTTGTACACTGCTGTTGCTTGGGGCCGCCCACAGGCCAGCACTTCCTGCGTA
  ATGCAGATGGAGCCACTTCCCATGCCCACCCGCAGGGCATCCACACCTGCATCAATGAGG
  TTCTTGGCCTGNGCAGCAGTGACCACATTGCCTCCAATGACTTGGAGATTAGGGTATTTG
  TCTTTGATGTACTTGATCATATTGATCTGGAAGATGGAAATTCCCTGGGAAGAGTNNCAA
  ACCACTACATCCACACAGCCTGGGCGAGCAAGTCAGCCTATACTGTCATCTNATGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000884
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000884.2, NP_000875.2
• RefSeq Size: 1712 bp
• RefSeq ORF: 1545 bp
• Locus ID: 3615
• Cytogenetics: 3p21.31
• Domains: CBS, IMPDH
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Gene Summary: 'This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]'