Integrin alpha 4 (ITGA4) (NM_000885) Human Untagged Clone

CAT#: SC119587

ITGA4 (untagged)-Human integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITGA4
• Synonyms: CD49D; IA4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000885 edited
  GAATTCGGCACGAGGCCTTCCTTTAGCCCGCTGGCGCCGGACACGCTGCGCCTCATCTCT
  TGGGGCGTTCTTCCCCGTTGGCCAACCGTCGCATCCCGTGCAACTTTGGGGTAGTGGCCG
  TTTAGTGTTGAATGTTCCCCACCGAGAGCGCATGGCTTGGGAAGCGAGGCGCGAACCCGG
  CCCCCGAAGGGCCGCCGTCCGGGAGACGGTGATGCTGTTGCTGTGCCTGGGGGTCCCGAC
  CGGCCGCCCCTACAACGTGGACACTGAGAGCGCGCTGCTTTACCAGGGCCCCCACAACAC
  GCTGTTCGGCTACTCGGTCGTGCTGCACAGCCACGGGGCGAACCGATGGCTCCTAGTGGG
  TGCGCCCACTGCCAACTGGCTCGCCAACGCTTCAGTGATCAATCCCGGGGCGATTTACAG
  ATGCAGGATCGGAAAGAATCCCGGCCAGACGTGCGAACAGCTCCAGCTGGGTAGCCCTAA
  TGGAGAACCTTGTGGAAAGACTTGTTTGGAAGAGAGAGACAATCAGTGGTTGGGGGTCAC
  ACTTTCCAGACAGCCAGGAGAAAATGGATCCATCGTGACTTGTGGGCATAGATGGAAAAA
  TATATTTTACATAAAGAATGAAAATAAGCTCCCCACTGGTGGTTGCTATGGAGTGCCCCC
  TGATTTACGAACAGAACTGAGTAAAAGAATAGCTCCGTGTTATCAAGATTATGTGAAAAA
  ATTTGGAGAAAATTTTGCATCATGTCAAGCTGGAATATCCAGTTTTTACACAAAGGATTT
  AATTGTGATGGGGGCCCCAGGATCATCTTACTGGACTGGCTCTCTTTTTGTCTACAATAT
  AACTACAAATAAATACAAGGCTTTTTTAGACAAACAAAATCAAGTAAAATTTGGAAGTTA
  TTTAGGATATTCAGTCGGAGCTGGTCATTTTCGGAGCCAGCATACTACCGAAGTAGTCGG
  AGGAGCTCCTCAACATGAGCAGATTGGTAAGGCATATATATTCAGCATTGATGAAAAAGA
  ACTAAATATCTTACATGAAATGAAAGGTAAAAAGCTTGGATCGTACTTTGGAGCTTCTGT
  CTGTGCTGTGGACCTCAATGCAGATGGCTTCTCAGATCTGCTCGTGGGAGCACCCATGCA
  GAGCACCATCAGAGAGGAAGGAAGAGTGTTTGTGTACATCAACTCTGGCTCGGGAGCAGT
  AATGAATGCAATGGAAACAAACCTCGTTGGAAGTGACAAATATGCTGCAAGATTTGGGGA
  ATCTATAGTTAATCTTGGCGACATTGACAATGATGGCTTTGAAGATGTTGCTATCGGAGC
  TCCACAAGAAGATGACTTGCAAGGTGCTATTTATATTTACAATGGCCGTGCAGATGGGAT
  CTCGTCAACCTTCTCACAGAGAATTGAAGGACTTCAGATCAGCAAATCGTTAAGTATGTT
  TGGACAGTCTATATCAGGACAAATTGATGCAGATAATAATGGCTATGTAGATGTAGCAGT
  TGGTGCTTTTCGGTCTGATTCTGCTGTCTTGCTAAGGACAAGACCTGTAGTAATTGTTGA
  CGCTTCTTTAAGCCACCCTGAGTCAGTAAATAGAACGAAATTTGACTGTGTTGAAAATGG
  ATGGCCTTCTGTGTGCATAGATCTAACACTTTGTTTCTCATATAAGGGCAAGGAAGTTCC
  AGGTTACATTGTTTTGTTTTATAACATGAGTTTGGATGTGAACAGAAAGGCAGAGTCTCC
  ACCAAGATTCTATTTCTCTTCTAATGGAACTTCTGACGTGATTACAGGAAGCATACAGGT
  GTCCAGCAGAGAAGCTAACTGTAGAACACATCAAGCATTTATGCGGAAAGATGTGCGGGA
  CATCCTCACCCCAATTCAGATTGAAGCTGCTTACCACCTTGGTCCTCATGTCATCAGTAA
  ACGAAGTACAGAGGAATTCCCACCACTTCAGCCAATTCTTCAGCAGAAGAAAGAAAAAGA
  CATAATGAAAAAAACAATAAACTTTGCAAGGTTTTGTGCCCATGAAAATTGTTCTGCTGA
  TTTACAGGTTTCTGCAAAGATTGGGTTTTTGAAGCCCCATGAAAATAAAACATATCTTGC
  TGTTGGGAGTATGAAGACATTGATGTTGAATGTGTCCTTGTTTAATGCTGGAGATGATGC
  ATATGAAACGACTCTACATGTCAAACTACCCGTGGGTCTTTATTTCATTAAGATTTTAGA
  GCTGGAAGAGAAGCAAATAAACTGTGAAGTCACAGATAACTCTGGCGTGGTACAACTTGA
  CTGCAGTATTGGCTATATATATGTAGATCATCTCTCAAGGATAGATATTAGCTTTCTCCT
  GGATGTGAGCTCACTCAGCAGAGCGGAAGAGGACCTCAGTATCACAGTGCATGCTACCTG
  TGAAAATGAAGAGGAAATGGACAATCTAAAGCACAGCAGAGTGACTGTAGCAATACCTTT
  AAAATATGAGGTTAAGCTGACTGTTCATGGGTTTGTAAACCCAACTTCATTTGTGTATGG
  ATCAAATGATGAAAATGAGCCTGAAACGTGCATGGTGGAGAAAATGAACTTAACTTTCCA
  TGTTATCAACACTGGCAATAGTATGGCTCCCAATGTTAGTGTGGAAATAATGGTACCAAA
  TTCTTTTAGCCCCCAAACTGATAAGCTGTTCAACATTTTGGATGTCCAGACTACTACTGG
  AGAATGCCACTTTGAAAATTATCAAAGAGTGTGTGCATTAGAGCAGCAAAAGAGTGCAAT
  GCAGACCTTGAAAGGCATAGTCCAGTTCTTGTCCAAGACTGATAAGAGGCTATTGTACTG
  CATAAAAGCTGATCCACATTGTTTAAATTTCTTGTGTAATTTTGGGAAAATGGAAAGTGG
  AAAAGAAGCCAGTGTTCATATCCAACTGGAAGGCCGGCCATCCATTTTAGAAATGGATGA
  GACTTCAGCACTCAAGTTTGAAATAAGAGCAACAGGTTTTCCAGAGCCAAATCCAAGAGT
  AATTGAACTAAACAAGGATGAGAATGTTGCGCATGTTCTACTGGAAGGACTACATCATCA
  AAGACCCAAACGTTATTTCACCATAGTGATTATTTCAAGTAGCTTGCTACTTGGACTTAT
  TGTACTTCTGTTGATCTCATATGTTATGTGGAAGGCTGGCTTCTTTAAAAGACAATACAA
  ATCTATCCTACAAGAAGAAAACAGAAGAGACAGTTGGAGTTATATCAACAGTAAAAGCAA
  TGATGATTAAGGACTTCTTTCAAATTGAGAGAATGGAAAACAGACTCAGGTTGTAGTAAA
  GAAATTTAAAAGACACTGTTTACAAGAAAAAATGAATTTTGTTTGGACTTCTTTTACTCA
  TGATCTTGTGACATATTATGTCTTCATGCAAGGGGAAAATCTCAGCAATGATTACTCTTT
  GAGATAGAAGAACTGCAAAGGTAATAATACAGCCAAAGATAATCTCTCAGCTTTTAAATG
  GGTAGAGAAACACTAAAGCATTCAATTTATTCAAGAAAAGTAAGCCCTTGAAGATATCTT
  GAAATGAAAGTATAACTGAGTTAAATTATACTGGAGAAGTCTTAGACTTGAAATACTACT
  TACCATATGTGCTTGCCTCAGTAAAATGAACCCCACTGGGTGGGCAGAGGTTCATTTCAA
  ATACATCTTTGATACTTGTTCAAAATATGTTCTTTAAAAATATAATTTTTTAGAGAGCTG
  TTCCCAAATTTTCTAACGAGTGGACCATTATCACTTTAAAGCCCTTTATTTATAATACAT
  TTCCTACGGGCTGTGTTCCAACAACCATTTTTTTTCAGCAGACTATGAATATTATAGTAT
  TATAGGCCAAACTGGCAAACTTCAGACTGAACATGTACACTGGTTTGAGCTTAGTGAAAT
  GACTTCTGGATAATTATTTTTTTATAATTATGGATTTCACCATCTTTCTTTCTGTATATA
  TACATGTGTTTTTATGTAGGTATATATTTACCATTCTTCCTATCTATTCTTCCTATAACA
  CACCTTTATCAAGCATACCCAGGAGTAATCTTCAAATCTTTTGTTATATTCTGAAACAAA
  AGATTGTGAGTGTTGCACTTTACCTGATACATGCTGATTTAGAAAATACAGAAACCATAC
  CTCACTAATAACTTTAAAATCAAAGCTGTGCAAAGACTAGGGGGCCTATACTTCATATGT
  ATTATGTACTATGTAAAATATTGACTATCACACAACTATTTCCTTGGATGTAATTCTTTG
  TTACCCTTTACAAGTATAAGTGTTACCTTACATGGAAACGAAGAAACAAAATTCATAAAT
  TTAAATTCATAAATTTAGCTGAAAGATACTGATTCAATTTGTATACAGTGAATATAAATG
  AGACGACAGCAAAATTTTCATGAAATGTAAAATATTTTTATAGTTTGTTCATACTATATG
  AGGTTCTATTTTAAATGACTTTCTGGATTTTAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000885 unedited
  NGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTTCCT
  TTAGCCCGCTGGCGCCGGNCACGCTGCGCCTCATCTCTTGGGGCGTTCTTCCCCGTTGGC
  CAACCGTCGCATCCCGTGCAACTTTGGGGTAGTGGCCGTTTAGTGTTGAATGTTCCCCAC
  CGAGAGCGCATGGCTTGGGAAGCGAGGCGCGAACCCGGCCCCCGAAGGGCCGCCGTCCGG
  GAGACGGTGATGCTGTTGCTGTGCCTGGGGGTCCCGACCGGCCGCCCCTACAACGTGGAC
  ACTGAGAGCGCGCTGCTTTACCAGGGCCCCCACAACACGCTGTTCGGCTACTCGGTCGTG
  CTGCACAGCCACGGGGCGAACCGATGGCTCCTAGTGGGTGCGCCCACTGCCAACTGGCTC
  GCCAACGCTTCAGTGATCAATCCCGGGGCGATTTACAGATGCAGGATCGGAAAGAATCCC
  GGCCAGACGTGCGAACAGCTCCAGCTGGGTAGCCCTAATGGAGAACCTTGTGGAAAGACT
  TGTTTGGAAGAGAGAGACAATCAGTGGTTGGGGGTCACACTTTCCAGACAGCCAGGAGAA
  AATGGATCCATCGTGACTTGTGGGCATAGATGGAAAAATATATTTTACATAAAGAATGAA
  AATAAGCTCCCCACTGGTGGTTGCTATGGAGTGCCCCCTGATTTACGAACAGAACTGAGT
  AAAAGAATAGCTCCGTGTTATCAAGAATATGTGAAAAAAATTTGGAGAAAATTNTGCATC
  ATGTCAAGCTGGAATATCCAGTTTTTACACAAAGGATTTAATTGTGATGGGGGCCCCAGG
  ATCATCTTACTGGACTGGCTCTCTTTTGTCTACAATATACTACAAATAAATACAAGCCTT
  TT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000885 unedited
  ATGAACTGCGGCCGCAATCTAGAGTCGAGTTACTTTTTTTTTTTTTTTTTAACTATCCAG
  AAAGTCTACTTAAAATAGAACCTCATATAGTATGAACAAACTATAAACTTATTCTAACTT
  TATATGAAAATTTTGCTGTCTTCCTAATTATATTACTTGTATACAAATTGAATCAGTATC
  TTTCAGCTAAATTTATGAATTTAAATTTATGAATTTTGTTTCTTCGTTTCCATGTAGCGT
  ATCACTTATTCTTGCAAAGGGACTACATAATATTACTTATAAGGAAATAGTTGATTGATA
  CTCATATATTTCTCATACATCATAATATATAATAAGCATAGGCCCCTTATTCTTTAGTAC
  ACTTTTGATTATATAGTTATTACTGAATTATCGTTTCTGTATTATCTAAAACAGCATGTA
  ACTACCAAAGTGCAACACATACAATCTTTTGTTTCAGAATATAACAAAAGATTTGAAGAT
  TACTCCTGGGATATTCTTGATCAAATTGTGTTATAGGTAGAATACTATAGGAAAAATATC
  ACATATCTACCTACATATAAACACCTGTATATATACAGAATGAAAGATGGTGAAATCACT
  AATTATTATCTAATAATTATCCATAAGTCATTTNACTAAGCTCAAACCAGTGTACATGTT
  CAATCTGAAGTTTGCCAGATTGGCCTATAATACTATATAATTCATAGCCTGATGANAAAA
  AATGGATGCTGGNACACAGCCCGTAGGAAATTATTATAAAACTAGGCTTTAAGTGATAAT
  GGTCACTCGTTATAAATTTGTGACAGCATTTTAAATATATATTTTTAAGAAATTCTTCCT
  CACGACNAAATAGAACTTGAAGAAACNNNGNNANCAGGGGATTAATTTANTGNNGGNGNN
  CTNGGNNNGNNNNNNNNAGNNNANNNNTNNNNNNAATTNANATNNATATTATTATCTGAA
  TTTNAGGGCCTACTTCTGACTAAAGAGCTTAATTTTTATAATTACAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000885
• Insert Size: 4760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000885.4, NP_000876.3
• RefSeq Size: 6082 bp
• RefSeq ORF: 3099 bp
• Locus ID: 3676
• Cytogenetics: 2q31.3
• Domains: FG-GAP
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: 'The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.