Plasma Kallikrein 1B (KLKB1) (NM_000892) Human Untagged Clone

CAT#: SC119592

KLKB1 (untagged)-Human kallikrein B, plasma (Fletcher factor) 1 (KLKB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLKB1
• Synonyms: KLK3; PKK; PKKD; PPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000892, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTTTATTCAAGCAAGCAACTTATTTCATTTCCTTGTTTGCTACAGTTTCCTGTGGATGTCTGACTC
  AACTCTATGAAAACGCCTTCTTCAGAGGTGGGGATGTAGCTTCCATGTACACCCCAAATGCCCAATACTG
  CCAGATGAGGTGCACATTCCACCCAAGGTGTTTGCTATTCAGTTTTCTTCCAGCAAGTTCAATCAATGAC
  ATGGAGAAAAGGTTTGGTTGCTTCTTGAAAGATAGTGTTACAGGAACCCTGCCAAAAGTACATCGAACAG
  GTGCAGTTTCTGGACATTCCTTGAAGCAATGTGGTCATCAAATAAGTGCTTGCCATCGAGACATTTATAA
  AGGAGTTGATATGAGAGGAGTCAATTTTAATGTGTCTAAGGTTAGCAGTGTTGAAGAATGCCAAAAAAGG
  TGCACCAGTAACATTCGCTGCCAGTTTTTTTCATATGCCACGCAAACATTTCACAAGGCAGAGTACCGGA
  ACAATTGCCTATTAAAGTACAGTCCCGGAGGAACACCTACCGCTATAAAGGTGCTGAGTAACGTGGAATC
  TGGATTCTCACTGAAGCCCTGTGCCCTTTCAGAAATTGGTTGCCACATGAACATCTTCCAGCATCTTGCG
  TTCTCAGATGTGGATGTTGCCAGGGTTCTCACTCCAGATGCTTTTGTGTGTCGGACCATCTGCACCTATC
  ACCCCAACTGCCTCTTCTTTACATTCTATACAAATGTATGGAAAATCGAGTCACAAAGAAATGTTTGTCT
  TCTTAAAACATCTGAAAGTGGCACACCAAGTTCCTCTACTCCTCAAGAAAACACCATATCTGGATATAGC
  CTTTTAACCTGCAAAAGAACTTTACCTGAACCCTGCCATTCTAAAATTTACCCGGGAGTTGACTTTGGAG
  GAGAAGAATTGAATGTGACTTTTGTTAAAGGAGTGAATGTTTGCCAAGAGACTTGCACAAAGATGATTCG
  CTGTCAGTTTTTCACTTATTCTTTACTCCCAGAAGACTGTAAGGAAGAGAAGTGTAAGTGTTTCTTAAGA
  TTATCTATGGATGGTTCTCCAACTAGGATTGCGTATGGGACACAAGGGAGCTCTGGTTACTCTTTGAGAT
  TGTGTAACACTGGGGACAACTCTGTCTGCACAACAAAAACAAGCACACGCATTGTTGGAGGAACAAACTC
  TTCTTGGGGAGAGTGGCCCTGGCAGGTGAGCCTGCAGGTGAAGCTGACAGCTCAGAGGCACCTGTGTGGA
  GGGTCACTCATAGGACACCAGTGGGTCCTCACTGCTGCCCACTGCTTTGATGGGCTTCCCCTGCAGGATG
  TTTGGCGCATCTATAGTGGCATTTTAAATCTGTCAGACATTACAAAAGATACACCTTTCTCACAAATAAA
  AGAGATTATTATTCACCAAAACTATAAAGTCTCAGAAGGGAATCATGATATCGCCTTGATAAAACTCCAG
  GCTCCTTTGAATTACACTGAATTCCAAAAACCAATATGCCTACCTTCCAAAGGTGACACAAGCACAATTT
  ATACCAACTGTTGGGTAACCGGATGGGGCTTCTCGAAGGAGAAAGGTGAAATCCAAAATATTCTACAAAA
  GGTAAATATTCCTTTGGTAACAAATGAAGAATGCCAGAAAAGATATCAAGATTATAAAATAACCCAACGG
  ATGGTCTGTGCTGGCTATAAAGAAGGGGGAAAAGATGCTTGTAAGGGAGATTCAGGTGGTCCCTTAGTTT
  GCAAACACAATGGAATGTGGCGTTTGGTGGGCATCACCAGCTGGGGTGAAGGCTGTGCCCGCAGGGAGCA
  ACCTGGTGTCTACACCAAAGTCGCTGAGTACATGGACTGGATTTTAGAGAAAACACAGAGCAGTGATGGA
  AAAGCTCAGATGCAGTCACCAGCATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000892 unedited
  GTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACCAGGTTCATTTTTA
  AGTGACAAGAGACTCACCTCCAAAAAGCAATTGTGTTTTCAGAATGATTTTATTCAAGCA
  AGCAACTTATTTCATTTCCTTGTTTGCTACAGTTTCCTGTGGATGTCTGACTCAACTCTA
  TGAAAACGCCTTCTTCAGAGGTGGGGATGTAGCTTCCATGTACACCCCAAATGCCCAATA
  CTGCCAGATGAGGTGCACATTCCACCCAAGGTGTTTGCTATTCAGTTTTCTTCCAGCAAG
  TTCAATCAATGACATGGAGAAAAGGTTTGGTTGCTTCTTGAAAGATAGTGTTACAGGAAC
  CCTGCCAAAAGTACATCGAACAGGTGCAGTTTCTGGACATTCCTTGAAGCAATGTGGTCA
  TCAAATAAGTGCTTGCCATCGAGACATTTATAAAGGAGTTGATATGAGAGGAGTCAATTT
  TAATGTGTCTAAGGTTAGCAGTGTTGAAGAATGCCAAAAAAGGTGCACCAGTAACATTCG
  CTGCCAGTTTTTTTCATATGCCACGCAAACATTTCACAAGGCAGAGTACCGGAACAATTG
  CCTATTAAAGTACAGTCCCGGAGGAACACCTACCGCTATAAAGGTGCTGAGTAACGTGGA
  ATCTGGATTCTCACTGAAGCCCTGTGCCCTTTCAGAAATTGGTTGCCACATGAACATCTT
  CCAGCATCTTGCGTTCTCAGATGTGGATGTTGCCAGGGTTCTCACTCCAGATGCTNNTTG
  TGTGTCGGACCATCTGCACCTATCACCCCAACTGCCTCTTCTTTACATTCTATACAAATG
  TATGGAAAATCGAGTCACCAGAAATGTTTGGTCTCTTTAAAACATCTGAAGTGGCACACC
  AAGTTCCTCTACTCCTCAAGAAACACATATCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000892 unedited
  AGTTTGNACGCGGCCGCAATTTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACTTTCACCCTTTTTTTTCACAACACC
  CAGTCTTGGGAAATTCAATTGGGACCCCCCATTTTCAACCCCTGGTTACGGGGGGCTTAA
  AAGGGGTTTTAACCAAAAATTGGCCCCAAAAACTTTGAAGGCCCCGGGGTTTTCCCCCTT
  TGGGGGCAAAAAAAAAGCCCCTTTCCATGCTTTGGAAAAAAAGACCCCCCCGGCTCAAAA
  TTTGACTTGAACCCAGGGGGGAAAAAATGCCTAAAATTTGGACGGGTTTTCATGGGGGGG
  AAGGGATTTGAACTTTTCCATCACTGGTTTGGGGTTTCTTTAAAAACCAACCCCTGTTCC
  CAACGACTTTGGGGGAAAAACCAAGTTGGTTCCTGGGGGGACAACCTTTACCCCAGTTGG
  GGAGGCCCACCAAACCCCACATTTCATTGGGTTTGGAAACCAAGGGACCCCCTGAATTTC
  CCTTAAAACATTTTTTCCCCCTTTTTTATAACCAACACAAACCAACCCGGGGGGTATTTT
  ATAAACTTGGGAATCTTTTCTGGAATTTTTATTTGTTACCAAAGGAATATTTACCCTTTG
  GAGAATATTTTGGAATCAACCTTTTTCCTTTGGAAAACCCCTTCGGGTACCCCAAAGTGG
  AAATAAAATGGGCTGGGGCACCTCTTGGAAAGGAGCTAATTGGGTTTTTGGATTCAAGGT
  AATTAAAGGGAGCTGGGAATTTATTAAGGGGAAATCAGGAATCCCCTCTGAAAATTTATA
  AGTTTGGGGAAAAAAAATCTTTATTTTGGAAAAAGGGTGTTTTTTGTATGTTCGGAGATT
  AAAGGCCCCTTATAAGGCCAAACATCTGCGGGGAAGCCTCAAACATGGGCAAATGAGACC
  CCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000892
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000892.2, NP_000883.1
• RefSeq Size: 2245 bp
• RefSeq ORF: 1917 bp
• Locus ID: 3818
• Cytogenetics: 4q35.2
• Domains: APPLE, Tryp_SPc, PAN
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: 'This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).