Kininogen 1 (KNG1) (NM_000893) Human Untagged Clone

CAT#: SC119593

KNG1 (untagged)-Human kininogen 1 (KNG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KNG1
• Synonyms: BDK; BK; HMWK; KNG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119593 sequence for NM_000893 edited (data generated by NextGen Sequencing)
  ATGAAACTAATTACCATCCTTTTCCTCTGCTCCAGGCTGCTACTAAGTTTAACCCAGGAA
  TCACAGTCCGAGGAAATTGACTGCAATGACAAGGATTTATTTAAAGCTGTGGATGCTGCT
  CTGAAGAAATATAACAGTCAAAACCAAAGTAACAACCAGTTTGTATTGTACCGCATAACT
  GAAGCCACTAAGACGGTTGGCTCTGACACGTTTTATTCCTTCAAGTACGAAATCAAGGAG
  GGGGATTGTCCTGTTCAAAGTGGCAAAACCTGGCAGGACTGTGAGTACAAGGATGCTGCA
  AAAGCAGCCACTGGAGAATGCACAGCAACCGTGGGGAAGAGGAGCAGTACGAAATTCTCC
  GTGGCTACCCAGACCTGCCAGATTACTCCAGCCGAGGGCCCTGTGGTGACAGCCCAGTAC
  GACTGCCTCGGCTGTGTGCATCCTATATCAACGCAGAGCCCAGACCTGGAGCCCATTCTG
  AGACACGGCATTCAGTACTTTAACAACAACACTCAACATTCCTCCCTCTTCACGCTTAAT
  GAAGTAAAACGGGCCCAAAGACAGGTGGTGGCTGGATTGAACTTTCGAATTACCTACTCA
  ATTGTGCAAACGAATTGTTCCAAAGAGAATTTTCTGTTCTTAACTCCAGACTGCAAGTCC
  CTTTGGAATGGTGATACCGGTGAATGTACAGATAATGCATACATCGATATTCAGCTACGA
  ATTGCTTCCTTCTCACAGAACTGTGACATTTATCCAGGGAAGGATTTTGTACAACCACCT
  ACCAAGATTTGCGTGGGCTGCCCCAGAGATATACCCACCAACAGCCCAGAGCTGGAGGAG
  ACACTGACTCACACCATCACAAAGCTTAATGCAGAGAATAACGCAACTTTCTATTTCAAG
  ATTGACAATGTGAAAAAAGCAAGAGTACAGGTGGTGGCTGGCAAGAAATATTTTATTGAC
  TTCGTGGCCAGGGAAACCACATGTTCCAAGGAAAGTAATGAAGAGTTGACCGAAAGCTGT
  GAGACCAAAAAACTTGGCCAAAGCCTAGATTGCAACGCTGAAGTTTATGTGGTACCCTGG
  GAGAAAAAAATTTACCCTACTGTCAACTGTCAACCACTGGGAATGATCTCACTGATGAAA
  AGGCCTCCAGGTTTTTCACCTTTCCGATCATCACGAATAGGGGAAATAAAAGAAGAAACA
  ACTAGTCACCTAAGGTCCTGCGAGTACAAGGGTCGACCCCCAAAGGCAGGGGCAGAGCCA
  GCATCTGAGAGGGAGGTCTCTTGA
  
  Clone variation with respect to NM_000893.3
  324 g=>a;533 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000893 unedited
  GGGTTCCCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACCAGGCCCTGAGT
  TCTGAGGCAGAGAGGAGGACAGAAGAAACAGAGGCTGGAGATTGTCAAATTCAGTATCCC
  AGTTGGCTCTTGATTCTTGGTGAAACCATCCCTCAGCTCCTAGAGGGAGATTGTTAGATC
  ATGAAACTAATTACCATCCTTTTCCTCTGCTCCAGGCTGCTACTAAGTTTAACCCAGGAA
  TCACAGTCCGAGGAAATTGACTGCAATGACAAGGATTTATTTAAAGCTGTGGATGCTGCT
  CTGAAGAAATATAACAGTCAAAACCAAAGTAACAACCAGTTTGTATTGTACCGCATAACT
  GAAGCCACTAAGACGGTTGGCTCTGACACGTTTTATTCCTTCAAGTACGAAATCAAGGAG
  GGGGATTGTCCTGTTCAAAGTGGCAAAACCTGGCAGGACTGTGAGTACAAGGATGCTGCA
  AAAGCAGCCACTGGAGAATGCACAGCAACCGTGGGGAAGAGGAGCAGTACGAAATTCTCC
  GTGGCTACCCAGACCTGCCAGATTACTCCAGCCGAGGGCCCTGTGGTGACAGCCCAGTAC
  GACTGCCTCGGCTGTGTGCATCCTATATCAACGCAGAGCCCAGACCTGGAGCCCATTCTG
  AGACACGGCATTCAGTACTTTAACAACAACACTCAACATTCCTCCCTCTTCACGCTTAAT
  GAAGTAAAACGGGCCCCAAAGACAGGTGGTGGGCTGGATTGAACTTTCGAATTACCTACT
  CAATTGTGCAAACGAATTGTTCCAAAGAGAAATTTCTGTTTCTAACTCCAGACTGCAAGT
  NCCCTTTGAATGGTGATACCGGNGAATGTACACGATATGCATACCTCGATATTCAGCTCC
  CAATGCTTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000893 unedited
  NTTACTCTGNACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTAAAACAACAAAACTGATCTTTATTTCACCCAAAGGCA
  GAGGGATAAAATGGCATTCTTCTCTATTTAAATTCTATCCCATCTTTCTTTTTGGCCTTC
  CTCTCAAGGGTGCTGGCAAAGGGGGTTGGGGCTATGTGCCTGGAGTGAAAATTCTGCCCA
  TTGGTCAAAAAACCTCCCTTTCAAATGCTGGCTCTGCCCCTGCCTTTGGGGGTCGACCCT
  TGTACTCCCAGGACCTTAGGGGACTAGTTGTTTCTTCTTTTATTTCCCCTATTCGGGATG
  ATCGGAAAGGGGAAAAACCTGGAGGCCTTTTCATCAGGGAAATCATTCCCAGGGGTTGAC
  AGTTGACAGTAGGGTAAATTTTTTTCTCCCAGGGTACCACATAAACTTCAGCGTTGCAAT
  CTAGGCTTTGGCCAAGTTTTTTGGTCTCACAGCTTTCGGNCAACTCTTCATTACTTTCCT
  TGGAACATGTGGTTTCCCTGGCCACGAAGTCAATAAAATATTTCTTGCCAGCCACCACCT
  GTACTCTTGCTTTTTTCACATTGNCAATCTTGAAAAAAAAGTTGCGTTATTCTCTGCATT
  AAGCTTTGTGAAGGTGTGAGATCANTGTCTCCTCCAACTCTGGGCTGTTGGGGGGTATAT
  CTCTGGGGCAGCCCACGCAATCTTGGGTAGGGGGTGTACAAAATCCTTCCTGGATAAATG
  TCACAGTTCTGGGAGAGGGAAGCATCCGAGCTGATATCGAGTATGCATTATCTGACATCC
  CCGTTTCACATCCAAGGGACTTGCATCTGGAGTAAAACAGAAATTCTTTTGGAACAATCG
  TTGCACCATGAGTAGGATTTCGAAGTCATTCAGCCACACTGGTTTGGGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000893
• Insert Size: 1690 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000893.2, NP_000884.1
• RefSeq Size: 1668 bp
• RefSeq ORF: 1284 bp
• Locus ID: 3827
• Cytogenetics: 3q27.3
• Domains: CY
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: 'This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reduces or depletes angiotensin converting enzyme 2 (ACE2), which results in an increase in levels of des-Arg(9)-bradykinin, a bioactive metabolite of bradykinin that is associated with lung injury and inflammation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2020]'
  Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region, compared to variant 1. The resulting isoform (2), also known as low molecular weight preprokininogen, has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.