HTR2C (NM_000868) Human Untagged Clone

CAT#: SC119615

HTR2C (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 2C (HTR2C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HTR2C
• Synonyms: 5-HT1C; 5-HT2C; 5-HTR2C; 5HTR2C; HTR1C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119615 sequence for NM_000868 edited (data generated by NextGen Sequencing)
  ATGGTGAACCTGAGGAATGCGGTGCATTCATTCCTTGTGCACCTAATTGGCCTATTGGTT
  TGGCAATGTGATATTTCTGTGAGCCCAGTAGCAGCTATAGTAACTGACATTTTCAATACC
  TCCGATGGTGGACGCTTCAAATTCCCAGACGGGGTACAAAACTGGCCAGCACTTTCAATC
  GTCATCATAATAATCATGACAATAGGTGGCAACATCCTTGTGATCATGGCAGTAAGCATG
  GAAAAGAAACTGCACAATGCCACCAATTACTTCTTAATGTCCCTAGCCATTGCTGATATG
  CTAGTGGGACTACTTGTCATGCCCCTGTCTCTCCTGGCAATCCTTTATGATTATGTCTGG
  CCACTACCTAGATATTTGTGCCCCGTCTGGATTTCTTTAGATGTTTTATTTTCAACAGCG
  TCCATCATGCACCTCTGCGCTATATCGCTGGATCGGTATGTAGCAGTACGTAATCCTGTT
  GAGCATAGCCGTTTCAATTCGCGGACTAAGGCCATCATGAAGATTGCTATTGTTTGGGCA
  ATTTCTATAGGTGTATCAGTTCCTATCCCTGTGATTGGACTGAGGGACGAAGAAAAGGTG
  TTCGTGAACAACACGACGTGCGTGCTCAACGACCCAAATTTCGTTCTTATTGGGTCCTTC
  GTAGCTTTCTTCATACCGCTGACGATTATGGTGATTACGTATTGCCTGACCATCTACGTT
  CTGCGCCGACAAGCTTTGATGTTACTGCACGGCCACACCGAGGAACCGCCTGGACTAAGT
  CTGGATTTCCTGAAGTGCTGCAAGAGGAATACGGCCGAGGAAGAGAACTCTGCAAACCCT
  AACCAAGACCAGAACGCACGCCGAAGAAAGAAGAAGGAGAGACGTCCTAGGGGCACCATG
  CAGGCTATCAACAATGAAAGAAAAGCTTCGAAAGTCCTTGGGATTGTTTTCTTTGTGTTT
  CTGATCATGTGGTGCCCATTTTTCATTACCAATATTCTGTCTGTTCTTTGTGAGAAGTCC
  TGTAACCAAAAGCTCATGGAAAAGCTTCTGAATGTGTTTGTTTGGATTGGCTATGTTTGT
  TCAGGAATCAATCCTCTGGTGTATACTCTGTTCAACAAAATTTACCGAAGGGCATTCTCC
  AACTATTTGCGTTGCAATTATAAGGTAGAGAAAAAGCCTCCTGTCAGGCAGATTCCAAGA
  GTTGCCGCCACTGCTTTGTCTGGGAGGGAGCTTAATGTTAACATTTATCGGCATACCAAT
  GAACCGGTGATCGAGAAAGCCAGTGACAATGAGCCCGGTATAGAGATGCAAGTTGAGAAT
  TTAGAGTTACCAGTAAATCCCTCCAGTGTGGTTAGCGAAAGGATTAGCAGTGTGTGA
  
  Clone variation with respect to NM_000868.2
  466 a=>g;478 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000868 unedited
  TTTTGGNNTTGTCGAAATTTTGTATACGACTCACTATAGGCGGCCCGCGATTCGGCACGA
  GGGAAGAAGCCAAACCTAGCCGGGGGGCGCACGGTCACCCAAGGAGGTCGACTCGCCGGC
  GCTTCCTATCGCGCCGAGCTCCCTCCATTCCTCTCCCTCCGCCGAGGCGCGAGGTTGCGG
  CGCGCAGCGCAGCGCAGCTCAGCGCACCGACTGCCGCGGGCTCCGCTGGGCGATTGCAGC
  CGAGTCCGTTTCTCGTCTAGCTGCCGCCGCGGCGACCGCTGCCTGGTCTTCCTCCCGGAC
  GCTAGTGGGTTATCAGCTAACACCCGCGAGCATCTATAACATAGGCCAACTGACGCCATC
  CTTCANAAACAACTGTCTGGGAAAAAAAGAATAAAAAGTAGTGTGAGAGCAGAAAACGTG
  ATTGAAACACGACCAATCTTTCTTCAGTGCCAAAGGGTGGAAAAGAAAGGGATGATATGA
  TGAACCTAGCCTGTTAATTNTCGTCTTCTCAATTTTAAACTTTGGGTGCTTAAGACTGAA
  GCAATCATGGTGAACCTGNAGAATGCGGNGCATTCATTCCTTGTGCACCTATTGGCCTAT
  TGGTTTGGCAATGTGATATTTCTGGGAGCCCAATAGCAGCTATAGGAACTGGCATTTTTC
  ATACCCTCCGAGGGGGGGACGCTTCCAAATNCCCAGACGGGGGACAAAACTGGGCAGCCC
  TTTCAATCGGCATCCTAAAATCATGACAAAAGGTGGCACATTCCTGGGATCATGGCGGGA
  AGCCTGGGAAAGAAACTGGCCATGGCCCCCATTACTTTTTAAGGCCCTAGCCACTGTGAA
  TGCCTATGGGACAACCTGGGATGCCCCTGGCCTTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000868 unedited
  TTGCATTACGACGACCCCGCTATCTACATGCAGCTCTTTTTTTTTTTTTTTCAACACTTA
  ATAGATTTAATAAATCAATAGGCAAGTCTCATAAAACCTGTAGTGCATAACATACAGGAA
  TTAGGAATAAACACAACACTTTGCAACCATTCACAAAAAACCCATTTGACAAAGGGAGAA
  GACTTACGGTAAATTAGTATAAGGCAGTCTGTTGCTCGTGTCTTACACAGATATACATAT
  ATAATAATACATCAAAATGATTCGTTTGATGGCAAATTCATCACCTTTACAATTATCACA
  TGGACTTGTTTAAAACATTTATAAAAGTTTGTAAATTTAACATTGAAGGGTAAGATTTAT
  ATCCAAACGAGTATATTTGTTTCATACCAAACTGCTCCAAACTTACGAGTAGCACACAAC
  TTGAGGATAATTACCAGGTTGGGCACTATTAAGTACTACTACTTGCTGACACAGGAATCA
  TTAACAAATGTGCTGTGAGCTATTATCATTTCACTTATTGCTATTACTTTTGGAAATCTG
  CGATTTATATTTTGTTCATTTGAAAAACATGGGCTATGAGCTTGCCCTGATAGTTCACAT
  ATCAGAGGTCTCCAGGTCTTGCTAACACTCACACTACTTCACACCTGAATGCATGGCCCT
  TTTTAAGGAAAGGCCGAACGGATTTCTCTTCCATCCCTTCTTTCTATAACCTTATTTCGC
  CCTACTTATAATACAAATCCAGGGGATTATTGCACCATCACATGGCCATACTTGCGAGCA
  ACCATCTTTNAGTGCCCCAACCTAAGGCTTAAAAGCATGGCCGGGCTACCTTTAAGCACA
  TTCTCATTTCCGGCAAAGAACAAAATGAATCAACTCTCGGCTCTCTTAAACGTATTTGTA
  ACATCCCCTTCAACAAAACCTAACTGCCCAGGGAGGAGAAAATGGACCTCCTGCTACAAT
  CTGGCACTGTCCCACAATATGCATACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000868
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000868.1, NP_000859.1
• RefSeq Size: 4775 bp
• RefSeq ORF: 1377 bp
• Locus ID: 3358
• Cytogenetics: Xq23
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants. [provided by RefSeq, Jan 2015]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). This variant may undergo RNA editing.