GAD67 (GAD1) (NM_000817) Human Untagged Clone

CAT#: SC119631

GAD1 (untagged)-Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAD1
• Synonyms: CPSQ1; GAD; SCP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119631 sequence for NM_000817 edited (data generated by NextGen Sequencing)
  ATGGCGTCTTCGACCCCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAAT
  ACCACTAACCTGCGCCCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACC
  AGAAAACTGGGGCTCAAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAG
  AGTCGCCTTGTGAGTGCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAA
  AACAGCGACCGGGATGCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCT
  AGAGATCTGCTTCCGGCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTG
  GTGGACATACTCCTCAACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGAC
  TTTCATCACCCACACCAGTTGCTGGAAGGCATGGAGGGCTTCAACTTGGAGCTCTCTGAC
  CACCCCGAGTCCCTGGAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGGGTT
  CGCACAGGTCATCCTCGATTTTTCAACCAGCTCTCCACTGGATTGGATATTATTGGCCTA
  GCTGGAGAATGGCTGACATCAACGGCCAATACCAACATGTTTACATATGAAATTGCACCA
  GTGTTTGTCCTCATGGAACAAATAACACTTAAGAAGATGAGAGAGATAGTTGGATGGTCA
  AGTAAAGATGGTGATGGGATATTTTCTCCTGGGGGCGCCATATCCAACATGTACAGCATC
  ATGGCTGCTCGCTACAAGTACTTCCCGGAAGTTAAGACAAAGGGCATGGCGGCTGTGCCT
  AAACTGGTCCTCTTCACCTCAGAACAGAGTCACTATTCCATAAAGAAAGCTGGGGCTGCA
  CTTGGCTTTGGAACTGACAATGTGATTTTGATAAAGTGCAATGAAAGGGGGAAAATAATT
  CCAGCTGATTTTGAGGCAAAAATTCTTGAAGCCAAACAGAAGGGATATGTTCCCTTTTAT
  GTCAATGCAACTGCTGGCACGACTGTTTATGGAGCTTTTGATCCGATACAAGAGATTGCA
  GATATATGTGAGAAATATAACCTTTGGTTGCATGTCGATGCTGCCTGGGGAGGTGGGCTG
  CTCATGTCCAGGAAGCACCGCCATAAACTCAACGGCATAGAAAGGGCCAACTCAGTCACC
  TGGAACCCTCACAAGATGATGGGCGTGCTGTTGCAGTGCTCTGCCATTCTCGTCAAGGAA
  AAGGGTATACTCCAAGGATGCAACCAGATGTGTGCAGGATACCTCTTCCAGCCAGACAAG
  CAGTATGATGTCTCCTACGACACCGGGGACAAGGCAATTCAGTGTGGCCGCCACGTGGAT
  ATCTTCAAGTTCTGGCTGATGTGGAAAGCAAAGGGCACAGTGGGATTTGAAAACCAGATC
  AACAAATGCCTGGAACTGGCTGAATACCTCTATGCCAAGATTAAAAACAGAGAAGAATTT
  GAGATGGTTTTCAATGGCGAGCCTGAGCACACAAACGTCTGTTTTTGGTATATTCCACAA
  AGCCTCAGGGGTGTGCCAGACAGCCCTCAACGACGGGAAAAGCTACACAAGGTGGCTCCA
  AAAATCAAAGCCCTGATGATGGAGTCAGGTACGACCATGGTTGGCTACCAGCCCCAAGGG
  GACAAGGCCAACTTCTTCCGGATGGTCATCTCCAACCCAGCCGCTACCCAGTCTGACATT
  GACTTCCTCATTGAGGAGATAGAAAGACTGGGCCAGGATCTGTAA
  
  Clone variation with respect to NM_000817.2
  111 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000817 unedited
  GCTCTTGCCCGCCCGTCGCCTCTAAGGGCGGTAGGCGTGTACGGTGGGNAGGTCTATATA
  AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG
  CGAATTCGGCACGAGGCGCGTGCGAGAGGGAACTAGCGAGAACGAGGAAGCAGCTGGAGG
  TGACGCCGGGCAGATTACGCCTGTCAGGGCCGAGCCGAGCGGATCGCTGGGCGCTGTGCA
  GAGGAAAGGCGGGAGTGCCCGGCTCGCTGTCGCAGAGCCGAGCCTGTTTCTGCGCCGGAC
  CAGTCGAGGACTCTGGACAGTAGAGGCCCCGGGACGACCGAGCTGATGGCGTCTTCGACC
  CCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAATACCACTAACCTGCGC
  CCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACCAGAAAACTGGGGCTC
  AAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAGAGTCGCCTTGTGAGT
  GCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAAAACAGCGACCGGGAT
  GCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCTAGAGATCTGCTTCCG
  GCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTGGTGGACATACTCCTC
  AACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGACTTTCATCACCCACAC
  CAGTTGCTGGAAGGCATGGGAGGCTTCAACTTGGAGCTCTCTGACCACCCCGAGTCCCTG
  GAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGAGTTCGCACAGGTCATCCT
  CGATTTTTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000817 unedited
  CGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTCAAATTCTCCATGGAGA
  ATATTTTTATTTATTTATATTCTTCTTGCATACTCTAAAACGCACAGTGTGAATTTCCCT
  TCTTCAAAAACAGTTGTGAGCCTGGTCACTTTATCTGAGATCTTCAGAAGGTCTTCGGAA
  ATGTTGCCTTAGGTTTCAGCTAAGCGAGTCACAGAGATTGGTCATCAACTACTACAAAAT
  TACAGGAGATCTAGTTATTATCCAACAGTTGCTAGAAAAGCATTGAGAACACCAGACGTA
  CAGCCAGACAGAGAGCGTAAGAGAAGAGCAGATGCTATTTAAGTAACAGGATTTCCATAT
  ATATGACTTTATGTACAATCTTAGAATTCTCTTACAGACAGTAAAATGCTTAAATCATTA
  TTTTAAGGGCACTCTAATATAAATACAAATCTCACTAATTTTAATCTTTTCTCTTTGGCT
  CACAAATGCACGAAAATACCATAAACAGTTTTCAGAACTTACTTCATATATAAATAAGAT
  ACATTACAAATGGGAAGAAAATACAAGACGCCTCTTTAACATGGTACATCTCTGAATATA
  AATAAGCAAATATTTCATTGACCTACAATTAAACAATCATTGAAATCATGACACATAGTC
  TCCCCCTCCCACCACCAAGGTCAGACAACAGGAATTTACACAATCTACACAAATTAATCT
  CTAAAAATACAGCCATAGTACACCTGGGTACATGACTCTGCTACTATTTTCCCCTACAGG
  ATACTAATGGGGNAGGGNTGTGACTGTCATTGGNNGGNNGTTTTTCTCATCCATCATCAC
  TTCAGGTGGAGAAACGCTGACAGTGAGGTCGATTCTTACCCAACAGCATTCCTTTGGCCN
  NCTAGACATGGCGTTTGAAAAACAGTTNCCTTGGAGTGTTGACAANCTGGNTGGCNCATA
  GTTANCTCCTCCGTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000817
• Insert Size: 3270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000817.2, NP_000808.2
• RefSeq Size: 3488 bp
• RefSeq ORF: 1785 bp
• Locus ID: 2571
• Cytogenetics: 2q31.1
• Domains: pyridoxal_deC
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus
• Gene Summary: 'This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]'
  Transcript Variant: Transcript variant GAD67 represents the full-length and predominant form of this gene.