GAD67 (GAD1) (NM_000817) Human Untagged Clone

CAT#: SC119631

GAD1 (untagged)-Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67


  "NM_000817" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GAD1
Synonyms CPSQ1; GAD; SCP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119631 sequence for NM_000817 edited (data generated by NextGen Sequencing)
ATGGCGTCTTCGACCCCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAAT
ACCACTAACCTGCGCCCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACC
AGAAAACTGGGGCTCAAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAG
AGTCGCCTTGTGAGTGCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAA
AACAGCGACCGGGATGCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCT
AGAGATCTGCTTCCGGCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTG
GTGGACATACTCCTCAACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGAC
TTTCATCACCCACACCAGTTGCTGGAAGGCATGGAGGGCTTCAACTTGGAGCTCTCTGAC
CACCCCGAGTCCCTGGAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGGGTT
CGCACAGGTCATCCTCGATTTTTCAACCAGCTCTCCACTGGATTGGATATTATTGGCCTA
GCTGGAGAATGGCTGACATCAACGGCCAATACCAACATGTTTACATATGAAATTGCACCA
GTGTTTGTCCTCATGGAACAAATAACACTTAAGAAGATGAGAGAGATAGTTGGATGGTCA
AGTAAAGATGGTGATGGGATATTTTCTCCTGGGGGCGCCATATCCAACATGTACAGCATC
ATGGCTGCTCGCTACAAGTACTTCCCGGAAGTTAAGACAAAGGGCATGGCGGCTGTGCCT
AAACTGGTCCTCTTCACCTCAGAACAGAGTCACTATTCCATAAAGAAAGCTGGGGCTGCA
CTTGGCTTTGGAACTGACAATGTGATTTTGATAAAGTGCAATGAAAGGGGGAAAATAATT
CCAGCTGATTTTGAGGCAAAAATTCTTGAAGCCAAACAGAAGGGATATGTTCCCTTTTAT
GTCAATGCAACTGCTGGCACGACTGTTTATGGAGCTTTTGATCCGATACAAGAGATTGCA
GATATATGTGAGAAATATAACCTTTGGTTGCATGTCGATGCTGCCTGGGGAGGTGGGCTG
CTCATGTCCAGGAAGCACCGCCATAAACTCAACGGCATAGAAAGGGCCAACTCAGTCACC
TGGAACCCTCACAAGATGATGGGCGTGCTGTTGCAGTGCTCTGCCATTCTCGTCAAGGAA
AAGGGTATACTCCAAGGATGCAACCAGATGTGTGCAGGATACCTCTTCCAGCCAGACAAG
CAGTATGATGTCTCCTACGACACCGGGGACAAGGCAATTCAGTGTGGCCGCCACGTGGAT
ATCTTCAAGTTCTGGCTGATGTGGAAAGCAAAGGGCACAGTGGGATTTGAAAACCAGATC
AACAAATGCCTGGAACTGGCTGAATACCTCTATGCCAAGATTAAAAACAGAGAAGAATTT
GAGATGGTTTTCAATGGCGAGCCTGAGCACACAAACGTCTGTTTTTGGTATATTCCACAA
AGCCTCAGGGGTGTGCCAGACAGCCCTCAACGACGGGAAAAGCTACACAAGGTGGCTCCA
AAAATCAAAGCCCTGATGATGGAGTCAGGTACGACCATGGTTGGCTACCAGCCCCAAGGG
GACAAGGCCAACTTCTTCCGGATGGTCATCTCCAACCCAGCCGCTACCCAGTCTGACATT
GACTTCCTCATTGAGGAGATAGAAAGACTGGGCCAGGATCTGTAA

Clone variation with respect to NM_000817.2
111 t=>c
>OriGene 5' read for NM_000817 unedited
GCTCTTGCCCGCCCGTCGCCTCTAAGGGCGGTAGGCGTGTACGGTGGGNAGGTCTATATA
AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG
CGAATTCGGCACGAGGCGCGTGCGAGAGGGAACTAGCGAGAACGAGGAAGCAGCTGGAGG
TGACGCCGGGCAGATTACGCCTGTCAGGGCCGAGCCGAGCGGATCGCTGGGCGCTGTGCA
GAGGAAAGGCGGGAGTGCCCGGCTCGCTGTCGCAGAGCCGAGCCTGTTTCTGCGCCGGAC
CAGTCGAGGACTCTGGACAGTAGAGGCCCCGGGACGACCGAGCTGATGGCGTCTTCGACC
CCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAATACCACTAACCTGCGC
CCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACCAGAAAACTGGGGCTC
AAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAGAGTCGCCTTGTGAGT
GCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAAAACAGCGACCGGGAT
GCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCTAGAGATCTGCTTCCG
GCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTGGTGGACATACTCCTC
AACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGACTTTCATCACCCACAC
CAGTTGCTGGAAGGCATGGGAGGCTTCAACTTGGAGCTCTCTGACCACCCCGAGTCCCTG
GAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGAGTTCGCACAGGTCATCCT
CGATTTTTCAA
>OriGene 3' read for NM_000817 unedited
CGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTCAAATTCTCCATGGAGA
ATATTTTTATTTATTTATATTCTTCTTGCATACTCTAAAACGCACAGTGTGAATTTCCCT
TCTTCAAAAACAGTTGTGAGCCTGGTCACTTTATCTGAGATCTTCAGAAGGTCTTCGGAA
ATGTTGCCTTAGGTTTCAGCTAAGCGAGTCACAGAGATTGGTCATCAACTACTACAAAAT
TACAGGAGATCTAGTTATTATCCAACAGTTGCTAGAAAAGCATTGAGAACACCAGACGTA
CAGCCAGACAGAGAGCGTAAGAGAAGAGCAGATGCTATTTAAGTAACAGGATTTCCATAT
ATATGACTTTATGTACAATCTTAGAATTCTCTTACAGACAGTAAAATGCTTAAATCATTA
TTTTAAGGGCACTCTAATATAAATACAAATCTCACTAATTTTAATCTTTTCTCTTTGGCT
CACAAATGCACGAAAATACCATAAACAGTTTTCAGAACTTACTTCATATATAAATAAGAT
ACATTACAAATGGGAAGAAAATACAAGACGCCTCTTTAACATGGTACATCTCTGAATATA
AATAAGCAAATATTTCATTGACCTACAATTAAACAATCATTGAAATCATGACACATAGTC
TCCCCCTCCCACCACCAAGGTCAGACAACAGGAATTTACACAATCTACACAAATTAATCT
CTAAAAATACAGCCATAGTACACCTGGGTACATGACTCTGCTACTATTTTCCCCTACAGG
ATACTAATGGGGNAGGGNTGTGACTGTCATTGGNNGGNNGTTTTTCTCATCCATCATCAC
TTCAGGTGGAGAAACGCTGACAGTGAGGTCGATTCTTACCCAACAGCATTCCTTTGGCCN
NCTAGACATGGCGTTTGAAAAACAGTTNCCTTGGAGTGTTGACAANCTGGNTGGCNCATA
GTTANCTCCTCCGTAC
Restriction Sites NotI-NotI     
ACCN NM_000817
Insert Size 3270 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000817.2, NP_000808.2
RefSeq Size 3488 bp
RefSeq ORF 1785 bp
Locus ID 2571
Cytogenetics 2q31.1
Domains pyridoxal_deC
Protein Families Druggable Genome
Protein Pathways Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus
Gene Summary 'This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]'
Transcript Variant: Transcript variant GAD67 represents the full-length and predominant form of this gene.

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